Lab report for experiment 10 for Chem 123 UBCFull description
Chem 123- UBC lab 10Full description
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EXPERIMENT 10 : SEPARATION OF A MIXTURE OF ALUM FERRIC AND AND COPPER SULPHATE SULPHATE USING USING COLUMN COLUMN (ELUTION) CHROMATOGRAPHY CHM 421
NAME : ANAS BIN MOHD KAMAL KAMAL ID
: 2016655!6
LECTURER"S NAME : DR# SUHAILA MOHAMAD MOHAMAD HANAPI
ABSTRACT
The purpose of this experiment is to separate a mixture of alum ferric and copper sulphate using column (elution) chromatography. The slurry of alumina
or
silica
was
used
as
stationary
phase
in
the
column
chromatography, while the mixture solution that is to be separated was the mobile phase. The mixture was let to elute until colours of bands shows up. From this experiment, the alum ferric and the copper sulphate had successfully separated by column chromatography.
OBJECTIVES
To separate a mixture of alum ferric and copper sulphate using column (elution) chromatography
INTRODUCTION
In this experiment, a mixture of alum ferric and copper sulphate was separated by elution chromatography. Elution chromatography is a technique used to separate components of a mixture by washing down the solutes through a column by additions of fresh solent. The mixture dissoles in a mobile phase, is added at the column head. The solutes in the mixture distribute themseles between the mobile phase and the stationary phase. Further partition of the solutes occurs as more eluent is poured down the column.
!olute is carried down the column by the mobile phase. Thus if the solute is more soluble in the mobile phase then it will spend more time in the mobile phase. The migration is therefore high for this solute. "onersely if
the solute is more strongly retained by the stationary phase then the migration rate is small. These di#erences in rates separate the components in the mixture into bands or $ones in the column. The separated bands can be isolated and further analysed by collecting the indiidual band as it passes out of the column.
METHOD
The slurry of alumina or silica was prepared by mixing the pac%ing material with water. It was started by weighing about &' g of the pac%ing material. Then, water is added to slightly aboe the leel of the powder. The mixture is mixed well. Then the slurry was poured slowly into a column & cm at a time. The column is pac%ed properly by %noc%ing the end of the column onto a soft padding (cloth, boo%, etc.) at the bench. The column was prepared until it is pac%ed to about *+ cm in length of alumina or silica. The column was clamped onto a retort stand. -lter paper was cut to -t into the mouth of the column. "arefully, the -lter paper was lowered down to rest aboe the pac%ing material. bea%er was placed underneath the column and the stopcoc% was opened to collect the water from the column. The column was neer let to dry. The water is always made sure to be about cm aboe the leel of alumina or silica. cm & of the gien mixture was added into the column. The components were eluted with distilled water until separation of the mixture occurs.
