B. Tech. Biotech Syllabus MDU

1

Bachelor of Technology ( Bio-Technology) Scheme of Courses/examination Sr. No.

Course

( 3rd Semester ) Teaching Schedule

Subject

Examination Schedule

No.

1 2 3 4 5 6 7 8 9

Duration of Exam.

BTT-201E Cell Biology BTT-203E Microbiology BTT-205E Biochemistry BTT-207E Ge Genetics BTT-209E Organic Chemistry BTTBTT-21 211E 1E Cell ell Bio Biolo logy gy and and Genetics Lab BTT-213E Microbiology Lab BTT-215E Biochemistry Lab BTT-217E Organic Chemistry Lab TOTAL

L

T

P/D P/D

TOTA TO TAL L

Th. Th. Sess Sess.. P/VV P/VV TO TOTA TAL L

3 3 4 3 3 -

1 1 1 1 1 -

4

4 4 5 4 4 4

100 100 100 100 100

16

5

3 4 3 14 14

3 4 3 35

500

50 50 50 50 50 50

-

50 50 50 450

50

150 150 150 150 150 100

3 3 3 3 3 4

50 50 50 50 200

100 10 1 00 100 1150

4 4 4

Bachelor of Technology ( Bio-Technology) Scheme of Courses/examination Sr.No.

Course

Subject

( 4th Semester ) Teaching Schedule


No.

1 2 3 4 5 6 7 8 9

BTT-202E Molecular Biology BTT-204E Immunology Bio-analytical BTT-206E Techniques BTT-208E Industrial Microbiology & Enzyme Technology BTT-210E Thermodynamics of Bio-processes BTT-212E Molecular Biology Lab BTT-214E Immunology Lab BTT-216E Bio-analytical Techniques Lab BTT-218E Industrial Microbiology Lab TOTAL

Duration of Exam.

L

T

P/D P/D

TOTA TO TAL L

Th. Th.

Sess Sess.. P/VV P/VV TO TOTA TAL L

4 3

1 1

-

5 4

100 100

50 50 50

-

150 150

3 3

3 3

1 1

-

4 4

100 100

50 50

-

150 150

3 3

3

1

-

4

100

50

-

150

3

-

-

4 3 3

4 3 3

-

50 50 50

50 50 50

100 100 10 100

4 4 4

-

-

4

4

-

50

50

10 1 00

4

16

5

14

35

500

450

200

1150

Students will undergo Practical Training of 6 weeks duration after the 4th Semester.

Scheme of Courses/Examination 2nd YEAR B. Tech. (Bio-Technology)

2

3rd Semester B. Tech. (Bio-Technology) CELL BIOLOGY BTT-201E L 3

T 1

Theory : 100 Marks Sessional : 50 Marks Total : 150 Marks Time : 3Hrs.

Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. Unit I 1. Cell: An introduction, classification of organisms by cell structure, cytosol, compartmentalization of eukaryotic cells, cell fractionation. 2. Cell membrane and permeability: Chemical components of biological membranes, organization and fluidity of membrane components, the membrane as a dynamic entity, cell signalling, cell recognition and membrane transport. Unit II 3. Cytoskeleton and cell motility : Structure and functions of microtubules, microfilaments, intermediate filaments. 4. Structure and Functions of Cellular Organelles: Endoplasmic reticulum, golgi complex, lysosomes, vacuoles and microbodies, ribosomes, mitochondria, plastids mitochondria, plastids .

Unit III 5. Nucleus: Structure, cell-cycle (interphase and M phases), regulation of cell cycle. 6. Extracel Extracellular lular matrix: matrix: Compositio Composition, n, molecules molecules that mediate mediate cell adhesion, adhesion, membrane receptors for extracellular matrix macromolecules, regulation of receptor expression and function.

Unit IV Different muscle muscle types in the body, structural structural proteins proteins of muscles, muscles, energetics and 7. Musc Muscle le cont contra ract ctio ion: n: Different regulation of muscle contraction. Restin ing g pote potent ntia ial, l, actio action n pote potent ntia ial, l, syna synapt ptic ic tran transm smis issi sion on,, 8. Neur Neuron onss and and neur neurot otra rans nsmi miss ssio ion: n: Rest neurotransmitters and receptors, the generation of action potential by sensory stimuli and mechanism of nerve-impulses. Text/ References Books:

1. Molec Molecul ular ar Bio Biolo logy gy of of cell cell,, 4thed. Alberts, Bruce ( et. al)(2002) Garland Science Publishing, New York.. 2. Cell BiologyBiology- Smith Smith and Wood by Chapman Chapman and and Hall. Hall. 3. Cell Biology: Biology: Organell Organellee structure structure and function, function, Sadava, Sadava, D E.(200 E.(2004) 4) Panima pub., pub., New Delhi. Delhi. th 4. Cell Cell and and Mole Molecu cular lar Bio Biolo logy gy,, 8 ed. Robertis, Edp De and Robertis, Emf De (2002) Lippincot Williams and Wilkins Pvt. Ltd.,(International Student Edition) Philadelphia. 5. Molec Molecul ular ar Cel Celll Bio Biolo logy gy 4th ed. Lodish, Harvey and .Baltimore, D(2000) W.H. fr eeman & Co. Newyork

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L T 3 1

3rd Semester B. Tech. (Bio-Technology) MICROBIOLOGY BTT-203E Theory : 100 Marks Sessional : 50 Marks Total : 150 Marks Time : 3Hrs.

Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT - I

Development of Microbiology, various branches 1. History and scope of Microbiology: Microbiology: Development of microbiology and applications of microbiology. Microbial Taxonomy Taxonomy-- criteria used including including molecular 2 . Classification Classification of Microorganism Microorganisms: s: Microbial approaches. Microbial phylogeny and current classification of bacteria. UNIT - II 3 . Microbial Diversity: Prokaryotes and Eukaryotes. Morphology and cell structure of major groups of microorganisms e.g. bacteria, fungi, algae, protozoa and viruses. Method odss of isol isolati ation on,, puri purific ficat atio ion n and and 4 . Cul tiv ati on and and Main aintena tenanc ncee of Micr icroor oorgani ganism sm:: Meth preservation. Pure culture technique and sterilization methods. Requireme ement nt for carbon carbon,, nitrog nitrogen, en, sulphu sulphurr and growth growth factors factors.. 5. Principle Principless of microbial microbial nutr nutrition ition:: Requir Nutritional categories of microorganisms.

UNIT - III 6 . Microbial Microbial Growth and Metabolism: Metabolism: Growth curve (normal and biphasic) and generation time. Measurement Measurement of growth. growth. Synchronou Synchronous, s, batch and continuou continuouss cultures. cultures. Metabolic Metabolic pathwayspathways- catabolic, catabolic, anabolic and amphibolic. Microbial fermentation and its types. 7 . Microbial Microbial Reproduction: Reproduction: S e x u a l a n d a s e x u a l r e p r o d u c t i o n ( t a k i n g a n e x a m p l e f r o m e a ch c h g r ou ou p) p ) . B a ct c t e ri r i a l r e co c o mb m b i na na t io i o n: n : t r an a n s fo f o rm r m a ti t i on o n , t r an a n s du du ct c t i on o n a n d c o nj nj u ga ga t io i o n. n. Formation of endospores and mechanism of sporulation. UNIT - IV 8. Environmental microbiology: Normal and contaminating microflora of water, soil and air. Methods to study water, soil and air pollution. Major water, air and soil borne microbial diseases. D e f i ni ni t i on o n , i m po p o r t a nt nt f e r me m e n t ed e d f o o ds ds a n d b e ve v e r a g es e s ( c u r d, d, 9 . Food Microbiology: Microbiology: yogurt, cheese, bread, idli, pickles, beer, wine). Factors effecting spoilage of food a nd food preservation methods. Methods to study food quality. Text/References Books: 1. M i c r o b i o l o g y 5 t h E di d i t io io n . P r es e s c ot ot t , L .M . M . ; H a rl r l e y, y, J .P . P . a nd n d K l ei e i n, n , D .A . A . (2 ( 2 00 0 0 3) 3) McGraw Hill, USA. 2. M ic i c ro r o bi b i ol o l og o g y. y . P e lc l c za z a r J r. r . , M .J .J .; . ; C h an a n , E. E . C. C . S. S . a n d Kr K r ie i e g, g , N .R . R . ( 19 1 9 93 9 3 ) Ta T a ta t a M c Gr G r aw aw Hill, New Delhi. 3. Food Microbiology 2 nd ed., Adam, M. R. and Moss (2003) Panima Pub, Pub, New Delhi. 4. The Handbo Handbook ok of Water Water and and Wastew Wastewater ater Microb Microbiol iology ogy Ed. Ed. Mara, Mara, D. and Horan, Horan, N. (2003) (2003) Academ Academic ic Press. 5. Mode Modern rn Food Food Mic Micro robi biol olog ogy. y. Jay Jay,, J.M. J.M. (199 (1996) 6) CBS CBS Publ Publis ishe hers rs and and Dist Distri ribu buto tors rs,, New New Delhi Delhi.. nd 6. Food Microb robiology: Fundamentals als and Frontiers ers 2 Editio Edition. n. Doyle, Doyle, M.P. M.P. Beucha Beuchat; t; L.R. L.R. and Montville, T.J. (2001) ASM Press Washington D.C.

4

3rd Semester B. Tech. ( Bio-Technology) BIOCHEMISTRY BTT-205E L 4

T 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT - I Introduction Introduction to Biochemistry : A Historical prospective prospective 2. Amino acids & Proteins – Structure Structure and properties of amino acids. Essential and non-essential amino acids. Peptide bonds. Types of proteins and their classification. Forces stabilizing protein structure and shape. Different levels of structural organization of proteins. Carbohydr ydrate ates-S s-Str truct ucture ure and fun funct ction ions: s: Stru 3. Carboh Struct ctur ures es and and prop proper erti ties es of mono monosa sacc ccha hari ride des, s, oligosaccharides and polysaccharides. Ring structure and mutarotation. Homo- and hetero-polysaccharides. Mucopolysaccharides . UNIT - II 4. Lipids- Structure and functions : Classification of lipids and their general functions. Essential fatty acids. Hydrolysis of fats, Saponification value, Rancidity of fats, Iodine number and Acid value. Cholesterolits structure and biological functions. 5. Nucleic Acids- Structure and functions : Structure and properties of purine and pyrimidine basis. Nucleosides and nucleotides. Biologically important nucleotides. 6. Enzymes : Nomenclatu Nomenclature re and classificatio classification n of Enzymes. Enzymes. Basic concept of holoenzym holoenzymes, es, apoenzymes apoenzymes,, cofactors, coenzymes, prosthetic groups, metalloenzymes, monomeric and oligomeric enzymes. Definitions of enzyme activity, specific activity and enzyme enzyme specificity. Role of NAD+/NADP+, FMN/FAD, coenzymes A, thiami thiamine ne pyroph pyrophos ospha phate, te, pyrido pyridoxal xal phosph phosphate, ate, lipoic lipoic acid, acid, biocyt biocytin, in, Vitami Vitamin n B 12 coenzy coenzymes mes and tetrahydrofolate in enzyme catalysis. UNIT-III 7. Carbohydrate Metabolism : Glycolysis. Fate of pyruvate under aerobic and anaerobic conditions. Pentose phosphate pathway pathway and its significance. Gluconeogenesis Gluconeogenesis pathway. Biosynthesis Biosynthesis of lactose, sucrose and starch. Glycogenolysis, glycogenesis and control of glycogen metabolism. Maintenance of blood glucose level. Energetics Energetics and regulation regulation of carbohydrate carbohydrate metabolism. metabolism. Glyoxylate Glyoxylate cycle. Photosynthes Photosynthesis is (light and dark reactions). 8. Lipid Metabolism : Beta -oxidation of saturated fatty acids, oxidation of unsaturated and odd carbon fatty fatty acid acids. s. Alph Alphaa and and omeg omegaa oxid oxidati ation on of fatt fatty y acids acids.. Form Format atio ion n and and util utiliz izati ation on of keto ketone ne bodi bodies es.. Degradation of triacylglycerols by lipases. Biosynthesis, elongation and desaturation of saturated fatty acids. Biosynthesis of triacylglycerols, phospholipids and cholesterol. UNIT - IV 9. Amino Acid Metabolism : General reactions of amino acids metabolism- transamination, oxidative and non-oxidative deamination and decarboxylation. General pathways of amino acids degradation. Urea cycle and its regulations. Nitrogen cycle. 10. Nucleic Acid Metabolism : Catabolism, de novo- biosynthesis and regulation of purine and pyrimidine nucleotides. Formation of deoxyribonucleotides. 11. Mitochondrial Mitochondrial ial electron electron transport transport chain. chain. Hypotheses Hypotheses of Mitochondrial oxidative phosphorylation: Mitochondr mitochondrial oxidative phosphorylation. Inhibitors and uncouplers of oxidative phosphorylation. 12. Integration of metabolisim – Basic concepts.

5

Text/ References Books: 1. Biochemistry, 4th edition, by L. Stryer (1995). W.H. Freeman Freeman & Co. NY th 2. Biochemistry, 4 edition, by G. Zubay (1998). Wm.C. Brown Publishers. 3. Biochemistry, 2nd edition, by Laurence A. Moran, K.G. Scrimgeour, H. R. Horton, R.S. Ochs and J. David Rawn (1994), Neil Patterson Publishers Prentice Hall. 4. Lehninger: Principles of Biochemistry, 3rd editio edition, n, by David David L. Nelson Nelson and M.M. M.M. Cox (2000) (2000) Maxmillan/ Worth publishers. 5. Biochemistry, 2nd edition, by R.H. Garrett and C.M. Grisham (1999) . Saunders college Publishing, NY. Sons, NY. 6. Fundam Fundament entals als of Bioche Biochemis mistry try by Donald Donald Voet Voet and Judit Judith h G Voet Voet (1999) (1999) , John John Wiley Wiley & Sons, Sons, NY th 7. Harper’s Bi Biochemistry, 25 25 edition, by R.K. Murray, P.A. Hayes, D.K. Granner, P.A. Mayes and V.W. Rodwell (2000). Prentice Hall International.

6

3 r d Semester B. Tech (Bio-Technology) GENETICS BTT-207E L T 3 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT -I

d e l a n d h i s e x p er e r i m en e n t s , m o no n o h yb yb r i d 1 . P ri ri n ncc iip p llee s o f H er e r ed e d iitt y a nd nd V ar a r iia a ti t i on o n : M e n de crosses, incomplete dominance and codominance, dihybrid crosses, multiple alleles( blood group systems), epistasis, lethal genes. Probability in prediction and analysis of genetic data. Pedigree analysis. chromosomes, cell division, division, sexual 2. Genes and Chromosomes: General features of chromosomes, r e p r od od u c ti ti o n . C h r o mo m o s om om a l t h e o r y o f i n he h e r i t an a n c e , s e x d e t e r m in in a t i on on . S e x -l - l i n ke ke d , s e x limited and sex-influenced inheritance. Variation in chromosome chromosome number and structure. UNIT- II 3. Molecular Molecular organization organization of chromosomes: chromosomes: Genome size and evolutionary complexity, s up u p er e r co c o il i l in i n g o f D NA N A , s tr t r uc u c tu t u re r e o f b ac a c te t e ri r i aall c hr h r om o m os o s om o m ee,, s tr t r uc u c tu t u re r e o f e uk u k ar a r yo y o ti ti c chromosome. Classification of mutations, mutations, spontaneous spontaneous mutations, 4. Gene Mutation and DNA Repair: Classification induced mutations, application of induced mutations, mutations, detection of mutations, site-directed mutagenesis, mechanisms of DNA repair. UNIT - III 5. Gene Linkage and Chromosome Mapping: Linkage and recombination of genes in a chromosome, chromosome, crossing over and genetic mapping, gene mapping by 2-point and three point test crosses. 6. Somatic Cell Genetics : Somatic cell hybrids production and gene mapping. UNIT - IV 7. Population Genetics and Evolution : Allele frequencies and genotype frequencies, random mating and Hardy-Weinberg Hardy-Weinberg principle. Inbreeding. Inbreeding. Genetics and evolution (Mutation and migration, natural selection, random genetics drift). 8. Quantitative Genetics : Quantitative Quantitative inheritance, causes of variation.

Text/ Reference Books: 1 . G e ne ne t ic i c s : A n a ly ly si s i s o f G e ne n e s a n d G e n om o m e s. s. 5 t h e d i t i o n ( 2 0 0 1 ) H a r t l , D . L . a n d J o n e s , E.W., Jones and Bartlet Publishers, Boston. 2 . G en e n et e t ic i c s. s . 5 t h edition (1998) Russell, P.J., Addison Wesley Longman, Inc., California. 3. Genetics: Analysis Analysis and and Principles. Principles. (1999) Brooker, R.J. R.J. McGraw Hill, Hill, New York. York . 4. Basic Genetics. Genetics. (2000) (2000) Miglani, Miglani, G.S., G.S., Narosa Narosa Publishing Publishing House, House, New Delhi.

7

3 r d Semester B. Tech (Bio-Technology) ORGANIC CHEMISTRY BTT-209E L T 3 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT-I 1. Types of Organic Reactions: Substitution, Substitution, Addition, Elimination Elimination reactions. WangerM eeee rw r w in i n r ea e a rr r r an a n ge g e me m e nt n t r ea e a ct c t io i o n. n . R eeaa rr r r an a n ge g e me m e nt n t s o f e le l e ct c t rro o n d ef e f ic i c ie i e nt n t n it i t rro o ge ge n atom(Hoffmann, Beckmann and Curtius). Hyperconjugation : concept and consequences. 2. IUPAC Nomenclature: Systematic IUPAC nomenclature of alkenes, alkynes, cycloalkanes, aromatics, bicyclic and polyfunctional organic compounds. Bond line notation . UNIT-II 3 . B o nd n d i n g : H y d ro r o g e n b o n d in i n g - N a t u r e , t y pe p e , s t a bi b i l i ty t y a n d i t s i m p or or t a nc n c e i n o r g an an i c compounds. compounds. p Π - d Π bonding. bonding. Ylids (S& (S& P) and Wittig reaction. reaction. Biological Biological methylating r ea e a ge g e nt n t s. s. T au a u to t o me m e ri r i sm s m -C - C on o n ce c e pt pt , R in i n gg - ch c h ai a i n t aau u to t o me m e r is is m, m, R in i n gg - ch c h ai a i n i so s o me m e ri r i sm sm , properties and reactions of keto-enol tautomers. l a s si si f ic i c a ti t i on o n o f s t er er e om o m e rs r s , d i as a s t er er e om o m e rs r s , s e pa pa r at a t i on on o f 4 . S te t e re re o C he h e mi m i st s t ry r y : C la e n a nt n t i o me m e r s , a b s ol ol u t e c o n fi f i g u ra r a t i on o n ( R & S ) , p r o je j e c t io i o n f o r mu m u l a e, e , s t e r eo eo c h em e m i s tr tr y o f c om o m po p o un u n ds d s c on o n ta t a in i n in i n g t wo w o a sy s y mm m m et e t rrii c C - a to t o ms m s , s te t e re r e oc o c he h e mi m i st s t rry y o f b ip i p he h e ny n y ls ls . Geometrical isomerism-concept, isomerism-con cept, E & Z nomenclature. nomenclature. 5. Carbonyl Compounds: Nature and structure of carbonyl group, Relative reactivities of carbonyl compounds, hydration and addition of alcohal to aldehydes and ketones. Addition of ammonia and ammonia derivatives to aldehydes and ketones. Wolf–Kishner reduction and its mechanism, mechanism, Aldol condensatio condensation, n, Claisen condensation condensation,, Reformatsky and Perkin reactions. UNIT -III 6. Acid Derivatives: Acid catalyzed and base catalyzed hydrolysis of esters and acid amides, ammonolysis and alcoholysis of esters, acid halides and acid anhydrides . 7. Polymers: Classificatio Classification n of polymers. polymers. Tacticity and functionali functionality, ty, mechanism mechanism of chain growth and step growth growth polymerizatio polymerization, n, coordinati coordination on polymerizatio polymerization. n. Preparation Preparation,, properties properties and uses of epoxy resins, PMMA. Natural rubber and its vulcanization. Elastomers-GR-S, GR-M and GR-I.Biomedical polymerssilicone rubber, polyurethanes & their applications UNIT-IV 8. Reducing Agents: Their applications in Organic Chemistry with special emphasis on LiAlH 4, NaBH4, Pt/Ni/H2, Metal/NH3 Solution, Hydroboration and Tri-n-butyl tin hydride. 9. Peptide Bond Synthesis : Protection of N-terminal and C-terminal of amino acids, formation of peptide bond, solid phase peptide synthesis.

and nucleophillic nucleophillic ring opening opening of epoxides. epoxides. 10. Epoxides: properties and their uses.

Crown ethers ethers and

8

Text/Reference Text/Reference Books:

Organic Chemistry V1:6th ed. Finar,I L(2003) L(2003) Pearson Education, Delhi

1.

2. Organic Chemistry V2:5th ed. Finar,I L(2003) L(2003) Pearson Education, Delhi. 3. Organic Chemistry 6th ed. Morrison,R & Boyd,T.(2003) Boyd,T.(2003) Pearson Education, Delhi. 4. Organic Chemistry. Paula Yurkanis Yurkanis Bruice ; Pearson Education, Delhi. 5.

Principle of Organic Synthesis. Richard Norman and James M Coxon.

6. Organic Chemistry :Reactions and Reagents,37th ed. O.P. Aggarwal (2003) Goel Publishing House, Meerut. 7. Organic Analytical Chemistry. Jagmohan Jagmohan (2003) Narosa pub. pub. New Delhi.

9

3rd Semester B. Tech. ( Bio-Technology) CELL BIOLOGY& GENETICS LAB BTT-211E L -

T -

P 4

Practical/V.V Sessional Total Time Note : A college must offer 70% of the below listed experiments. The remaining modified by college according to facilities available .

: 50 Marks : 50 Marks : 100 Marks : 4 Hrs. 30% experiments may be

1. Microscopy: Microscopy: Structure Structure of of Prokaryo Prokaryotic tic and and eukaryo eukaryotic tic cell, cell, Fixation, Microtomy. Histol Histology ogy of variou variouss organ organ system systemss (Nervou (Nervous, s, digest digestion ion,, reprod reproduct uctive ive,, respir respirato atory ry and circulatory system). Mitochondrial staining and enzyme localization (Histochemistry and immunohistochemistry ). 2. Cell Cell divis division ion in onion onion root root tip. tip. 3. Cell Cell divisi division on in inse insect ct gonad gonads/f s/flow lower er bud. bud. 4. Fluore Fluoresce scence nce labeli labeling ng of cellul cellular ar organel organelles les.. 5. Isolation Isolation of DNA and study study of its denaturation denaturation spectrop spectrophotom hotometrically etrically and viscome viscometricall trically. y.

Reference books:

1. Prin Princi cipl ples es and and tech techni niqu ques es of Prac Practi tical cal Bioc Bioche hemi mist stry ry:: K. Wils Wilson on and and J. Wa Walk lker er (199 (1994) 4),, Camb Cambri ridg dgee University Press, Cambridge. 2. Introd Introduct uctory ory practica practicall Bioche Biochemis mistry try by S.K. Sawhney Sawhney and Randhir Randhir Singh Singh (2000), (2000), Narosa Narosa Publis Publishin hing g House, New Delhi. 3. An introductio introduction n to Practical Biochemistr Biochemistry y by David T. Plummer Plummer (1988), (1988), McGraw- Hill, Book Book company, company, UK.

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3rd Semester B. Tech. ( Bio-Technology) MICROBIOLOGY LAB BTT-213E L -

T -

P 3

Practical/V.V Sessional Total Time

: 50 Marks : 50 Marks : 100 Marks : 4 Hrs.

Note : A college must offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available. 1. M i c r o s c o p y : Use of microscopes, microscopic examination of microorganisms. 2. M i c r o m e t r y : Microscopic measurement of microorganisms. 3. Staining methods. 4. P rree pa pa rra a ttii on o n of o f cu c u lltt ur ur e m eed d iia a. 5. I s o la la t i on on a nd n d e nu nu m e ra ra t i on on o f m i cr c r o or o r g an a n i s ms ms f ro r o m d if if f er e r e nt n t s ou ou r ce ce s . 6. P u re re cu c u l tu tu r e te t e c h n i qu qu es es - Streak plate, pour plate, spread plate. 7. M ea e a s ur ur em e m e nt nt s o f g ro r o wt w t h a nd n d s t u dy dy o f e f fe f e ct c t o f v a ri r i o us us f a ct c t or o r s o n g ro r o w th th o f microorganisms -temperature, pH, salt concentration, U.V and R.H. 8. B i oc oc he h e mi m i c al al t es e s ts t s u se se fu f u l in i n b ac ac te t e ri r i a l t ax a x on o n o my my . multiple-tube fermentation test. 9. W a t e r Mi M i c r o b i o l o g y - BOD, multiple-tube 1 0. 0 . M il i l k Mi Mi ccrr o ob b iio o lo l o gy g y - SPC, testing the quality of milk. References Books: 1. E x pe p e r i m en en t s i n M i c r ob ob i ol o l o g y, y, P l a nt nt P a t ho ho l o gy gy a n d B i o te te c h no n o l og o g y . 4 t h Edition. Aneja, K.R. (2003)New Age International Publishers, New Delhi . Microbiology- a laboratory manual. 4 th edition. Cappuccino J. and Sheeman N. 2. (2000) Addison Wesley, California. Environmental Environmental Microbiology – A Laboratory Manual Pepper. I.L.; Gerba, C.P. and 3. Brendecke, J.W.(1995) Academic Press, New York.

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3rd Semester B. Tech. ( Bio-Technology) BIOCHEMISTRY LAB BTT 215E L -

T -

P 4



Note : A college must offer 70%of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available..

1. 2. 3. 4. 5. 6.

Qualitative Qualitative tests tests for amino amino acids, acids, proteins, proteins, Lipids Lipids and carbohyd carbohydrates. rates. Quantitativ Quantitativee estimation estimation of of proteins, proteins, Lipids Lipids and carbohy carbohydrates drates.. Assay Assay of of any comm commonl only y occurr occurring ing enzy enzyme. me. Effect of pH, pH, temperature, temperature, enzyme enzyme concentrati concentration on and protein protein denaturati denaturation on on an enzyme enzyme activity. activity. Dete Determ rmin inat atio ion n of of K m and Vmax of any commonly occurring enzyme. Biochemical Biochemical analysis analysis of urine and blood(p blood(pH, H, Uric acid, creatinine, creatinine, proteins proteins and carbohyd carbohydrates). rates).

Reference Books: 1. Princi Principle pless and techniq techniques ues of Practi Practical cal Bioche Biochemis mistry try:: K. Wilson Wilson and J. Walker Walker (1994) (1994),, Cambri Cambridge dge University Press, Cambridge. Introductory ry practical Biochemistry Biochemistry by S.K. Sawhney and Randhir Singh (2000), (2000), Narosa Publishin Publishing g 2. Introducto House, New Delhi. 3. An introduction to Practical Biochemistry by David T. Plummer (1988), McGraw- Hill, Book company, UK.

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3rd semester B. Tech. ( Bio-Technology) Bio-Technology)

ORGANIC CHEMISTRY LAB BTT-217E

L T P - - 3



Note : A college must offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available

proteins. 1. Derivitizations of poly sugars such as agarose with cynogen bromide for binding with proteins. 2. Derivitization of polystyrene to generate carboxyl groups. 3. Derivitization of polystyrene to generate amino groups. 4. Derivitization of polystyrene to generate aldehyde groups. 5. Derivitization of polysugars to generate aldehyde group and establish covalent linkage to protein through amino and carboxyl groups.

a s polyester, glass, polystyrene to provide amino groups. 6. Sialinization of solid phases such as 7. Covalent linkage of proteins to solid phase through carbodiiamide reaction.

amino, aldehyde and carboxyl group on solid-phase/liquid phase. 8. Estimation of reactive groups such as amino, 9. Preparation of aspirin from salicyclic acid. 10. Preparation of anthranilic acid from phthalic anhydride. 11. Preparation of p-nitro acetanilide from aniline.

fr om p-aminophenol. 12. Preparation of phenacetin from

13

L T 4 1

4th Semester B. Tech. (Bio-Technology) (Bio-Technology) MOLECULAR BIOLOGY BTT-202E Theory : 100 Marks Sessional: 50Marks Total : 150 Marks Time : 3Hrs.

Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit.

Unit –I 1. Genes : DNA/RNA as the genetic material. Double helical structure of DNA. Types of DNA. Super coiling and periodicity of DNA. Linking number of DNA. Nature of multiple alleles, Cis- acting sites and Trans–acting molecules. Euchromatin and heterochromatin. Nucleosomes. Organelle DNA- Mitochondrial and chloroplast DNA. 2. From Genes to Genomes : Gene mapping, exons and introns, repetitive and non –repetitive DNA, value paradox.

C-

Origin of DNA replication. replication. Bacterial Bacterial and eukaryotic replicons. replicons. DNA polymerases polymerases.. 3. DNA Replication : Origin Mechanism and regulation of DNA replication in prokaryotes and eukaryotes. UNIT - II 4. Tr Tran ansc scri ript ptio ion: n: Various RNA species and their properties- tRNA as an adapter and turnover of mRNA. Transcri Transcriptio ption n in Prokaryo Prokaryotes: tes: RNA polyme a) polymeras rases. es. Mechani Mechanism sm of transcri transcripti ptiononinitiation, elongation and termination. Role of sigma factor in transcription. Transc Transcrip riptio tion n in Eu Eukar karyot yotes: es: RNA b) RNA Poly Polyme mera rase ses. s. Down Downst stre ream am and and upst upstrea ream m promoters. promoters. Techniques Techniques to define promoterspromoters- foot printing printing experiment. experiment. Mechanism of transcriptio transcription. n. Inter Interac actio tion n of upst upstre ream am facto factors rs with with basa basall appa appara ratu tus. s. Role Role of enha enhanc ncers ers.. Post Post-t -tran ransc scri ript ptio ional nal modifications of various RNA species. Transcription in mitochondria and chloroplast. c) Positi Positive ve and negati negative ve contro controll of transc transcrip riptio tion, n, repres repressor sor-in -induc ducer er The Oper Operon: on: complex, catabolite repression and attenuation. Regulation of Transcription: DNA binding d) binding domain domainss- zinc finger finger motif, motif, helix loop helix, leucine zippers and homeodomains. Demethylation and gene regulation. UNIT - III 5. Genetic Code: Evid Eviden ence ce for for trip triple lett code code.. Prop Proper erti ties es of gene geneti ticc code code,, Wobb Wobble le hypo hypoth thes esis is.. Mitochondrial genetic code. Suppressor tRNAs. 6. Prot Protei ein n Synt Synthe hesi siss : Structure of prokaryotic and eukaryotic ribosomes and their role in protein synthesis synthesis.. Mechanism Mechanism of initiatio initiation, n, elongation elongation and and termination termination of protein protein synth synthesis. esis. Regulat Regulation ion of translation in prokaryotes and eukaryotes. Post tra nslational modifications of proteins. 7. Prot Protei ein n ffol oldi ding ng : Role of molecular chaperones. UNIT - IV 8. Nucl Nucleear Splic plicin ing g : Lariat formation, formation, Sn RNAs, cis-splicin cis-splicing g and trans-splicing trans-splicing reactions. reactions. Catalytic Catalytic RNA- Ribozymes- Ribonuclease P, small RNAs, group I &II introns. 9. Transposons: Tran Transp spos osit itio ion n by repli replicat cativ ivee and and non non repl replic icat ative ive mech mechan anis isms ms.. Inte Interm rmed ediat iates es of transposition. Retroviruses and retroposons.

14

10. 10. Cell Cell Cyc Cycle and and Growt owth Regu Regula lattion ion : Diffe Differen rentt stag stages es of cell cell cycl cycle. e. Cont Contro roll of cell cell cycle cycle by phosphorylation and dephosphorylation dephosphorylation mechanisms. Basic concept of Apoptosis. Apoptosis. Text/Reference Text/Reference Books :

1. Genes Genes VII, Lewin, Lewin, Benjam Benjamin( in(200 2002)O 2)OUP, UP, Oxford Oxford.. nd 2. Genomes,2 ed, Brown, T. T. A.(2002) John Wiley and sons ,Oxford 3. Mole Molecu cula larr biol biolog ogy y of of cell cell 4thed Alberts, Bruce; Watson,J D(2002) Garland Science Publishing, New York. 4. Molecular Molecular cell biology biology 4th ed Lodish, Lodish, Harvey and. and. Baltimore,D Baltimore,D(2000 (2000)) W.H. Freeman Freeman and Co., New York 5. Cell Cell and and Molec Molecul ular ar Biol Biolog ogy y 8th ed, Robertis, EDP De & Robertis, EMF De(2002) lippincott Williams & Wilkins international student edition, Philadelphia. 6. Essent Essentials ials of Molecu Molecular lar Biolog Biology y 4th ed, Malacinski, G. M. (2003) Jones & Bartlet Publishers, Boston 7. Cell Cell and and Molec Molecul ular ar Biol Biolog ogy: y: conc concep epts ts and and exper experim imen ents ts 3 rd ed Karp, Karp, Gerald(200 Gerald(2002) 2) John Wiley and sons, New York. 8. The Cell Cell-a -a molec olecul ular ar app approac roach, h, 3rd ed Cooper, G M& Hausman, R E(2004) ASM Press, Washington D C

15

4th Semester B. Tech. (Bio-Technology) (Bio-Technology) IMMUNOLOGY BTT-204E L T 3 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT - I 1. Introduction immunity, cells and organs of immune systemsystem- BIntroduction to immune system: Innate and acquired immunity, Lymphocytes and T-Lymphocytes, primary and secondary lymphoid organs, humoral and cell mediated immune response. 2. Immune System: Antigens. Immunoglobulins- structure and function, antigenic determinants(isotype, allotype, idiotype). Monoclonal antibodies. Hybridoma technology. Antibody engineering. UNIT - II

3. Antibody Diversity: Organization and expression of immunoglobulin genes, generation of antibody diversity; class switching. 4. Generation of B-Cell and T-Cell Responses : Major histocompatibility complex. Antigen processing and presentation. 7. Cell mediated immunity: T-cell receptor, T-cell maturation, activation and differentiation. UNIT - III

8. Immunological techniques : Immunoprec Immunoprecipitin ipitin reactions, reactions, agglutinatio agglutination n reactions, reactions, complement complement tests, tests, ELISA, RIA, Immunofluorescence. 9. Immune effector responses : Cytokines. Complement system. UNIT - IV

8. Immune System in Health and Disease : Hypersensitive reactions. Auto immunity and immune response to infectious diseases. Tumor Tumor immunity. Immune response to transplants. Vaccines. Text / Reference Books: 1. Kuby’s Im Immunology, 5 th ed. Goldsby, R A,. Kindt, T.J, Osborne, B.A.(2003) W. H. Freeman and company, New York. 2. Essential Immunology, 10 th ed Roitt, Roitt, Ivon; Delves, Peter(2001) Peter(2001) Blackwell Blackwell Scientific Scientific Publicatio Publications ns Oxford. 3. Fundam Fundament entals als of Immu Immunol nology ogy:: Paul Paul W.E. W.E. (Eds (Eds.) .) Rave Raven n Press Press,, New New York York.. 4. Immunology by by Press esscot.

16

L 3

T 1

4th Semester B. Tech. ( Bio-Technology) BIO-ANALYTICAL BIO-ANALYTICAL TECHNIQUES BTT-206E Theory : 100 Marks Sessional : 50 Marks Total : 150 Marks Time : 3Hrs.


UNIT- I Light, electron electron (scanning (scanning and transmissi transmission), on), phase phase contrast, contrast, fluorescence fluorescence microscopy, microscopy, 1. Microsco Microscopy: py: Light, atomic force microscopy, freeze-fracture techniques, specific staining of organelles or marker enzymes. 2. Centrifugation: Centrifugation: Techniques and their applications, differential centrifugation, zonal, density gradient and ultracentrifugation techniques. UNIT- II Paper and gel electro electropho phores resis, is, immuno immunoelec electro tropho phores resis, is, isoelec isoelectric tric focuss focussing ing,, twotwo3. Electrop Electrophore horesis sis: Paper dimensional electrophoresis. 4. Chromatography Chromatography: Paper, TLC, adsorption, partition, ion-exchange, reverse phase, gel filtration, affinity, gas chromatography, High Pressure Liquid Chromatography (HPLC). UNIT- III 5. Spectrophotometry: Spectrophotometry: Basic concepts and brief description of applications of UV/visible, IR, NMR, ESR, fluorescence, Raman. Mass spectroscopy (LC-MS, MALDI-TOF, ES-MS) X-ray diffraction (diffraction by fibrous proteins, globular proteins and molecular crystals), CD and ORD. 6. Calorimetry: Differential scanning calorimetry, titration calorimetry. UNIT- IV 7. Radioisotope Techniques: Nature of radioactivity, properties of α , β and γ -rays, measurement of radioactivity, use of radioisotopes in research, In vivo and in vitro labelling techniques, double labelling, instruments for monitoring radioactivity, quenching, internal standard, channel ratio, external standard ratio, emulsion counting, radioactive decay, autoradiography, radio-immunoassay.

Text/ References Books: 1. Biolog Biological ical Spect Spectros roscop copy: y: Campbe Campbell ll and Durek Durek nd 2. Phys Physic ical al Bio Bioch chem emis istr try, y, 2 edition, by D Friefelder (1983). W.H. Freeman & Co., U.S.A. 3. Introd Introduct uction ion to instru instrumen mental tal analys analysis: is: Robert Robert D. Braun (1987) (1987) Mc Graw Graw Hill Hill Intern Internatio ational nal Editions Editions,, Chemistry Series. 4. Analytical Analytical Chemistry Chemistry for technici technicians ans : John Kenkel Kenkel (1994), (1994), Lewis Lewis Publisher Publishers. s. Boca Raton. Raton. 5. Prin Princi cipl ples es and and tech techni niqu ques es of Prac Practi tical cal Bioc Bioche hemi mist stry ry:: K. Wils Wilson on and and J. Wa Walk lker er (199 (1994) 4),, Camb Cambri ridg dgee University Press, Cambridge. 6. Biophy Biophysic sical al Chemistr Chemistry: y: Princip Principles les and Techniq Techniques ues,, 2 nd edition by A. Upadhyay, K. Upadhyay and N. Nath. (1998). Himalaya Publishing House, Delhi. 7. Phys Physic ical al Bio Bioch chem emis istr try, y, 2nd edition, by K. E. VanHolde (1985), Prentice Hall Inc, New Jersey.

17

L T 3 1

4 t h Semester B. Tech. ( Bio-Technology) INDUSTRIAL MICROBIOLOGY AND ENZYME TECHNOLOGY BTT-208E Theory : 100 Marks Sessional : 50 Marks Total : 150 Marks Time : 3Hrs.

Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT- I 1. Industrial Microbiology: Introduction, objectives and scope . 2. Fermentation Technology: Principle, range and component of fermentation proc esses. Types of fermentation. Purification of fermentation products. products . 3 . I n du d u s t ri ri a l ly ly i m p or or t an a n t m i c ro ro b es e s : S o u rc r c e s , i s o la l a t i on on , s c r e en en i n g, g, p r e se se r v a ti ti o n a n d m a in i n te t e n an a n ce c e o f i nd n d us u s t ri r i a ll l l y i m po po r ta ta n t m ic i c r oo o o r ga ga n is is ms m s . I m pr pr o ve ve me m e n t o f i nd n d us u s t ri r i a ll ll y important microorganisms, selection of mutants, use of rDNA technology. UNIT - II 4 . P r oc o c e ss s s t e ch c h n o lo lo g y f o r t h e P r od o d u c ti ti o n o f v a ri r i o u s P r od o d u c ts ts : Primary metabolites (ethanol, acetone, butanol, citric acid, vinegar). Production of alcoholic beverages (wine and beer). 5. Microbial production of industrial enzymes : Cellulase, amylase and protease. i b i ot o t i c s ( e.g. penicillin, streptomycin and 6. Production of secondary metabolites : A n t ib tetracycline) UNIT - III 7. Vaccines : Types of vaccines and their production 8. Biopesticides: Biopesticides: C h a ra r a c t er e r i s t ic i c s o f b i op o p e s ti t i c i de d e s . I m p or or t a nt n t b i o pe pe s t ic i c i d e ss- B t - to t o x i n, n, Kasugamycin, Beauverin, Devine and Collego 9. Microbial protein: Quorn si c c o nc nc e pt p t s a nd n d i mp m p or o r t an a n t t yp y p e s o f b io i o f ue ue l s a nd nd 1 0 . B i o fu fu e ls l s a n d b i o fe fe r ti ti l i ze z e r s : B a si biofertilizers UNIT - IV Nomenclature and Classification Classification of enzymes. enzymes. Mechanism of of enzyme action, 11. Enzymes: Nomenclature acid base catalysis, covalent catalysis proximity and orientation effects. Mechanism of a c t i on on o f s e l ec e c t e d e n z ym y m e s - c h y mo mo t r yp y p s in i n , l y s oz oz y m e a n d r i b on o n u c le le a s e . P u r if i f i c a ti ti o n o f enzymes. Immobilized enzymes. Stability of enzymes- enzyme stabilization by selection a n d p r o te te i n e n gi g i n e e ri r i n g. g . A p p li li c a t io io n o f e n z ym ym e s i n i n du d u s t ry r y , a n a ly l y t i ca c a l p u r po po s es es a n d medical therapy. Text/Reference Books: 1 . I n du du st s t r ia i a l M i c r ob ob i ol ol o gy gy . C a s i d a J r . , L . E . ( 1 9 6 8 ) N e w A g e I n t e r n a t i o n a l ( P ) L t d . N e w Delhi. 2 . P r es e s c ot ot t & D un u n n’ n ’ s I n du du s tr tr i al a l M i cr c r o bi bi ol o l o gy gy . E d. d . E .G . G . R e ed e d ( 1 98 98 7) 7) . C B BS S P ub u b l is is h er er s , New De lhi. 3. Bi ot ec hn ol og y: A Text Textbo book ok of of Indu Indust stri rial al Micr Microb obio iolo logy gy 2nd Edition. Crueger, W. and Crueger, A. (2000) Panima Publishing Corporation, New Delhi. 4 . E nz nz ym ym es e s : B io i o ch ch em e m is i s tr t r y, y , B io i o te t e ch c h no no lo l o gy gy , C li l i ni ni ca c a l c he h e mi m i st s t ry r y . P aall me me r, r , T . ( 20 2 0 00 00 ) Horwood publishing Colphon. 5. Pro ce ss eng ine er ing in bio tec hno log y. Jack Jackso son n, A.T. .T. (19 (1991) 91) Pren Prenti tice ce Hall Hall.. nd 6 . M a nu nu a l o f I n du du st s t r ia i a l M i cr c r ob o b i ol ol o gy gy a nd n d B i ot ot e ch c h no n o lo lo g y 2 E di d i t io i o n. n . E d. d . A r no no l d L . Demain and Julian E. Davies (1999) ASM Press Washington D.C.

18

4th Semester B. Tech. (Bio-Technology) (Bio-Technology) THERMODYNAMICS THERMODYNAMICS OF BIO-PROCESSES BTT-210E L T 3 1

Theory : 100 Marks Sessional : 50 Marks

Total Time

: 150 Marks : 3Hrs.


UNIT- I 1.

2. 3. 4.

2. 3. 4.

Concept of Open, Closed, Closed, adiabatic and isolated systems with suitable examples. Biological Biological System as open systems. Thermodynamic parameters –internal energy, enthalpy ; their relationship and their significance. First law of thermodynamics. thermodynamics. Kirchoff’s Equation. Equation. Heat capacity at constant pressure and volume and their relationship. Concep Concepts ts of Free energy energy and Entro Entropy py,, Second Second law of thermody thermodynam namics. ics. Entro Entropy py change changess for reversible and irreversible processes. Entropy of mixing. Thir Third d Law Law of Ther Thermo mody dyna nami mics cs.. Entro Entropy py & life life proc proces esse ses. s. Nume Numeric rical al prob proble lems ms on Laws Laws of Thermodynamics. UNIT -II 1. Basic concept of Equilibrium and steady state conditions, Free energy and its relation relation with equilibrium equilibrium constant, constant, Chemical Chemical potential, potential, Gibbs-Duhe Gibbs-Duhem m equation equation and their application application,, Standard Standard biochemical biochemical state and standard standard free energy changes. Thermodyn Thermodynamic amic basis of Biochemical reactions. Phase Equilib Equilibrium rium and phase phase rule (thermodyn (thermodynamic amic derivation) derivation),, Free energy of transfer transfer between between phases. Structural transition in biological macromolecules macromolecules and molecular processes. Binding – independent and non-cooperative non-cooperative binding, Co-operative binding binding and its biological significance. UNIT -III

1.

Biological application of thermodynamics. thermodynamics. Concept of coupled reactions and group group transfer potentials. Thermodynamic analysis of some important metabolic pathways. 2. Concept of flux and forces. forces. Non-equilibrium Non-equilibrium thermodynamics and its biological biological applications. applications. 3. Coupled flows and Onsager’s phenomenological co-efficients and reciprocal relations. 4. Membrane-types and transport across biomembranes. UNIT- IV

1. Prigogine and Prigogine-Curie law. Thermo analysis of oxidative phosphorylation. 2. Stability of non equilibrium stationary state. 3. Ordering in time and space far from equilibrium. 4. Biological significance of the thermodynamic properties of water. 5. Biochemical oscillations and Biological clocks. Text/Reference Text/Reference Books : 1. Kinetics and Thermodynamics in Biochemistry : Bray & White. 2. Biophysical chemistry Vol. I : Edsall and Wyman 3. Non Equilibrium Thermodynamics in Biophysics : Katchalasky and Curran; Harvard University Press. 4. Physical Biochemistry : Van Holde 5. Physical basis basis of biochemistry : Foundations of molecular biophysics, Bergethan, P.R.(2000) P.R.(2000) NY, Springer. 6. Introduction to the thermodynamics thermodynamics of biological processes : Jou D.& Llebot J.E., Prentice Hall, New Jersey.

19

7. Biochemical Engineering Fundamentals, 2nd ed., Bailey J.E.; Ollis D.F.(1986) MGH, New York.

L -

T -

P 4

4th Semester B. Tech. (Bio-Technology) (Bio-Technology) MOLECULAR BIOLOGY LAB BTT-212E Practical/V.V : 50Marks Sessional : 50Marks Total : 100Marks Time : 4Hrs

Note : A college must offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available .

1. Isolat Isolation ion of genom genomic ic DNA from from eukaryo eukaryotic tic cells. cells. 2. Isolat Isolation ion of of RNA from from euka eukaryo ryotic tic cells cells.. 3. Isolat Isolation ion of protei proteins ns from from eukary eukaryoti oticc cells. cells. 4. Isolation Isolation of genomic genomic DNA DNA from from prokary prokaryotic otic cells. 5. Isolat Isolation ion of plasm plasmid id DNA from from Proka Prokaryo ryotic tic cells. cells. 6. Restriction Restriction mappin mapping g of plasmid plasmid DNA:Thi DNA:Thiss experiment experiment involve involvess single single and double double digesti digestion on of the plasmid with restriction enzymes. 7. Gel electrophor electrophoretic etic separation separation of DNA and molecular molecular wt. determinatio determination. n. 8. Gel electro electropho phoreti reticc separat separation ion of RNA. RNA. 9. Gel electr electroph ophore oretic tic separat separation ion of prote proteins ins.. 10. Transblot Transblot analysis analysis of DNA. 11. Gel Extractio Extraction n of DNA. 12. PCR amplification of DNA: Visualization by by gel electrophoresis. electrophoresis.

Reference Book: Molecular Cloning – A laboratory manual: 3 rd Edition Vol. 1-3. Sambrook J and Russell D.W. (2001). Cold Spring Harbor laboratory Press, New York.

20

4th Semester B. Tech. (Bio-Technology) (Bio-Technology) IMMUNOLOGY LAB BTT-214E L T P Practical/V.V : 50Marks - 3 Sessional : 50Marks Total : 100Marks Time : 4Hrs experiments. The remaining 30% experiments experiments may Note : A college must offer 70%of the below listed experiments. be modified by college according to facilities available.

1. Routin Routinee techni technique quess in handli handling ng laborato laboratory ry animals: animals: feeding feeding,, cleani cleaning ng and bleeding bleeding proced procedure ure for mice and rabbit. 2. Surgic Surgical al removal removal of lymph lymphatic atic organ organss from mice. mice. 3. Preparation Preparation and and administrati administration on of antigens, antigens, followin following g immunization immunization protocol protocols. s. 4. To bleed bleed rabbits rabbits for for the generation generation of antibod antibodies. ies. 5. Purific Purificati ation on of of immun immunogl oglobu obulin lins. s. 6. Isolation Isolation and purification purification of lymph lymphocytes ocytes from mouse. mouse. 7. Immuno Immunopre precipi cipitati tation on techniq techniques ues 8. Aggl Agglut utin inat atio ion n techni techniqu ques es 9. ELISA

Reference Books:

1. Using Antibo Antibodies: dies: A Laborator Laboratory y Manual. Harlow Harlow & Lane(19 Lane(1998) 98) Cold Spring Spring Harbor Harbor Lab Press. Press. 2. Immunologi Immunological cal Techniques Techniques Made Easy. Easy. Cochet, Cochet, et al.(1998)Wile al.(1998)Wiley y Publishers,Ca Publishers,Canada. nada.

21

4th Semester B. Tech. ( Bio-Technology) BIO-ANALYTICAL TECHNIQUES LAB

BTT-216E L -

T -

P 3

Practical/V.V : 50Marks Sessional : 50Marks Total : 100Marks Time : 4Hrs

Note : A college must offer 70%of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available.

1. 2. 3. 4.

Verifi Verificati cation on of BeerBeer-Lam Lamber bert’s t’s law. Separation Separation of amino amino acids/ acids/ sugars sugars by paper paper chromato chromatograph graphy. y. Extraction Extraction of of lipids lipids from tissues tissues and their separat separation ion using using TLC. TLC. Part Partia iall purif purific icat atio ion n of an enzy enzyme me by ammo ammoni nium um sulp sulpha hate te fract fractio iona natio tion, n, Ion Ion exch exchan ange ge and and gel gel filtration chromatography of proteins. 5. Determinatio Determination n of molecular molecular weight weight of of an enzyme enzyme by by gel filtratio filtration. n. 6. Separat Separation ion of prot protein einss by SDSSDS-PAG PAGE. E. 7. Cell Cell fra fract ctio iona nati tion on Reference Books: 1. Princi Principle pless and techniq techniques ues of Practi Practical cal Biochemi Biochemistr stry: y: K. Wilson Wilson and J. Walker Walker (1994), (1994), Cambridg Cambridgee University Press, Cambridge. 2. Introducto Introductory ry practical practical Biochemis Biochemistry try by S.K. S.K. Sawhney Sawhney and and Randhir Randhir Singh Singh (2000), (2000), Narosa Publishing Publishing House, New Delhi. 3. An intro introdu ducti ction on to Prac Practi tical cal Bioc Bioche hemi mist stry ry by Davi David d T. Plum Plumme merr (198 (1988) 8),, McGra McGraww- Hill Hill,, Book Book company, UK.

22

4th Semester B. Tech. ( Bio-Technology) INDUSTRIAL MICROBIOLOGY LAB BTT-218E L -

T -

P 4

Practical/V.V : 50Marks Sessional : 50Marks Total : 100Marks Time : 4Hrs Note : A college must offer 70%of the below listed experiments. experiments. The remaining 30% experiments experiments may be modified by college according to facilities available.

1 . S t e r il il i z a ti t i o n T ec e c h n iq i q u e s ( Me M e d i a, a , a i r & w at at e r ) 2 . C o ns n s t r uc u c t i on o n o f v a r i o us us f e r m e nt nt e r s ( b i or or e a c to to r s ) 3. Identificatio Identification n of industr industrially ially import important ant microorga microorganisms nisms e.g. molds, molds, yeasts yeasts and bacteria. bacteria. 4. Production Production of various various produ products cts in the the lab. lab. Alcohol, Alcohol, wine, wine, cellulase, cellulase, protease protease and bread. 5. Isolation Isolation of antibiotic antibiotic produc producing ing microorg microorganism anismss from the the soil. soil. 6. Penicillin Penicillin produc production tion and testing testing of antimicro antimicrobial bial activity activity.. 7. Isolation Isolation of strepto streptomycin mycin-resist -resistant ant mutants mutants by replica replica plating method method.. 8. Isolat Isolation ion of UV indu induced ced auxot auxotrop rophic hic mutan mutants. ts. 9 . T e s ti t i n g of o f m i c r ob ob i a l e nz nz y m e a ct c t i v it it y i n t he he l a b . 10. D et e r mina ti on of c e ll gr owt h. Reference Books:

1. Experiments in Microbiology, Plant Pathology and Biotechnology. Aneja, K.R. (2003) 4 t h Edition. New Age International Publishers, New Delhi. 2. F er e r m en en t at at io i o n s & B i oc oc he h e mi m i c al al H an a n d B o ok ok : P ri r i n ci ci p le le s, s , P ro r o ce c e ss s s D es e s i gn gn a n d Equipment . HC Vogel and Noyes(1983). cr o bi b i o l og o g y L a b or or t ar a r y M a nu nu a l . C a pp 3. M i cr pp uc u c c in i n o, o , J . a n d S he h e e ma m a n, n , N . (2 (2 0 00 00 ) , 4 t h Edition, Addison Wesley, California. 4. Manual of Industrial Microbiology and Biotechnology. 2 n d Edition. Ed. Arnold L. Demain and Julian E. Davies (1999) ASM Press Washington D.C.

23

Scheme of Courses/Examination 3rd YEAR B. Tech. (Bio-Technology) Bachelor of Technology (Bio-Technology) Scheme of Courses/Examination Courses/Examination (5th Semester)

Sr. No.

Course No.

1

BTT-301 E

2

BTT-303 E

3

BTT-305 E

Subject Recombinant DNA Technology Bioreactor Analysis and Design Bioprocess

Teaching Schedule L

T

P/D

3

1

-

3

1

3

TOTAL

Examination Schedule P/VV

TOTAL

Duration

Th.

Se Sess.

of Exam.

4

100

50

-

150

3

-

4

100

50

-

150

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

-

-

4

4

-

50

50

100

4

-

-

4

4

-

50

50

100

4

-

-

3

3

-

50

50

100

4

-

-

-

-

-

50

-

50

4

18

6

11

600

500

150

12 1250

Engineering 4

BTT-307 E

Downstream Processing Diagnostic

5

BTT-309 E

Techniques Biostatistics &

6

BTT-311 E

Computer Applications

7

BTT-313 E

8

BTT-315 E

9

BTT-317 E

10

BTT-319 E

r-DNA Tech. Lab Fermentation & Downstream Processing Lab Diagnostic techniques & Biostatistical analysis Lab Training Evaluation (viva-voce) TOTAL

35 35

Bachelor of Technology (Bio-Technology.) Scheme of Courses/Examination Courses/Examination (6th Semester)

Sr. No.

Course No.

1

BTT-302 E

2

BTT-304 E

3

4 5 6 7 8

9

Subject Microbial Biotechnology Plant

Biotechnology Animal & Aquaculture BTT-306 E Biotechnology BTT-308 E

Healthcare

Biotechnology Environmental BTT-310 E Biotechnology BTT-312 E

Food

Biotechnology Cell & Tissue BTT-314 E Culture Lab BTT-316 E

Healthcare

Biotechnology Lab Microbial, Food & BTT-318 E Environmental Biotechnology Lab

Teaching Schedule L

T

P/D

3

1

-

3

1

3

TOTAL

Examination Schedule P/VV

TOTAL

Duration

Th.

Sess.

of Exam.

4

100

50

-

150

3

-

4

100

50

-

150

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

3

1

-

4

100

50

-

150

3

-

-

4

4

-

50

50

100

4

-

-

4

4

-

50

50

100

4

-

-

3

3

-

50

50

100

4

24 10

BTT-320 E Seminar TOTAL

-

-

-

-

18

6

11

35 35

Students will undergo Practical Training of 6 weeks duration after 6th semester.

-

50

-

600

500

150

50 1250

25

5th Semester B. Tech. (Bio - Technology) Recombinant DNA Technology BTT-301 E L 3

T 1



UNIT I Tools Tools of Recomb Recombina inant nt DNA: DNA: Restri Restrictio ction n endonu endonuclea cleases ses.. Plasmi Plasmid d clonin cloning g vector vectors. s. Creati Creating ng and screening screening a gene library. Genetic Genetic transformat transformation ion of prokaryotes prokaryotes.. Cloning Cloning DNA sequences sequences encoding eukaryotic proteins. Vectors for cloning large pieces of DNA.

Chemical al synthe synthesis sis of DNA. DNA. DNA Chemical Chemical synthesi synthesis, s, sequenci sequencing ng and amplifica amplification tion of DNA: Chemic sequencing sequencing techniques. techniques. PCR. Analysis of eukaryotic eukaryotic DNA by chromosom chromosomal al walking. walking. Southern Southern and Northern Blotting. In situ hybridization.

UNIT II Isolation of cloned genes: Basic strategies for cloning. Developing improved bacteria and vectors. Prob Probes es to loca locate te clon clones es and and relat related ed gene genes. s. Iden Identi tifi ficat catio ion n and and isol isolat atio ion n of tiss tissue ue speci specifi ficc cDNA cDNA.. Procedures to analyze proteins encoded by cDNA clones. DNA markers: RFLP. RAPD and DNA fingerprinting.

UNIT III Study of gene functions: Directed mutagenesis. Identification of mutant clones. Use of PCR to construct genes encoding chimeric proteins. Mutagenesis-gateway to gene function and protein engineering.

UNIT IV Application of recombinant DNA in biotechnology: In medicine and Industry: Production of small biomolecules: vitamin-C, amino acids and indigo. Production Production of insulin, human growth hormone and its variants. Hepatitis-B virus vaccine. Tailoring Tailoring antibodies for specific applications. Biopolymers production. Marshalling recombinant DNA to fight AIDS.

26

Text/ References Books:

1. Reco Recom mbin binant ant DNA DNA 2 nd Edition. Watson, James D. and Gilman, M. (2001) W.H Freeman and Company, New York. 2. Mole Molecu cula larr Biot Biotech echno nolo logy gy:: Principles Application of Recombinant DNA 2nd Edition. Glick, B. R. and Pasternak, J. J. (1998) ASM press Washington DC. 3. Genetic Genetic Engineeri Engineering. ng. Ahluwalia, Ahluwalia, K. B. (2002) (2002) New Age Internati International onal (P) (P) Ltd. Ltd. nd 4. An Intro Introdu duct ctio ion n to Geneti Geneticc Engi Engine neeri ering ng 2 edition Desmond Nicholl S.T. (2002) Cambridge University Press. 5. Gene Geneti ticc Engi Engine neeri ering ng:: An introduction to Gene analysis and exploitation in eukaryotes . Kingsman and Kingsman (1998) Blackwell Scientific Publication, Oxford. 6. DNA DNA clon clonin ing g: A Practical Approach. Glover and Hames (2001) Oxford Univ. Press.

27

5th Semester B. Tech. (Bio - Technology) Bioreactor Analysis and Design

BTT-303 E L

T

Theory

: 100 Marks

3

1

Sessional : 50 Marks Total : 150 Marks Time : 3Hrs.


UNIT – I Types of reactor: Batch, plug flow reactor (PFR), continuous stirred tank reactor (CSTR), Fluidized bed reactor, bubble column, air lift fermenter, mechanical mechanical design of bioreactors. bi oreactors.

Concept of ideal and non ideal reactors, residence time distribution, models of non ideal reactors – plug flow with axial dispersion, tanks-in-series model, chemostat model with cell growth kinetics.

UNIT - II Plug flow reactor: For microbial processes, optimization of reactor systems.

Multiphase bioreactors: bioreactors: Packed Packed bed with with immobi immobiliz lized ed enzyme enzymess or microb microbial ial cells, cells, three three phase phase fluidized bed trickling bed reactor, design and analysis of above reactor systems.

UNIT – III Unconventional Unconventional bioreactors: bioreactors: Gas liquid liquid reacto reactors rs,, hollow hollow fiber fiber reacto reactor, r, membra membrane ne react reactor or and perfusion reactor for animal and plant cell culture UNIT – IV

Bio Reactors High Performance: Performance: Sterile and non sterile operations - Reactors in series with and without recycle. Design of Reactors. Reactors for Solid state fermentation. Text/Reference Text/Reference Books:

Landfi Landfill ll Bioreac Bioreactor tor Design Design & Operati Operation on.. Reinha Reinhart rt Debra Debra R, Towns Townsend end Timoth Timothy y G. and Townse Townsend nd Tim(1997) Lewis Publishers, Inc. Multiphase Bioreactor Design. Edited by: Joaquim M.S. Cabral, Manuel Mota, Johannes Tramper (2001) CRC Press. 2. Bioreactor & Ex Situ Biological Treatment Technologies – 5. Allerman Bruce, Allerman Bruce C, Leeson Andrea, (1999). Battelle publisher. 1.

Bioreac Bioreaction tion Engine Engineerin ering: g: Modelin Modeling g & Contro Control. l. vol. vol. I&II. I&II. Schuge Schugerl rl K, and Bellga Bellgardt rdt K.H, K.H, (2000) (2000),, Springer Verlag pub.

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5th Semester B. Tech. (Bio - Technology) Bioprocess Engineering BTT-305 E L 3

T 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT– I Introduction: History and role of bioprocess engineering in biotechnology industries, Concept of unit operation unit processes. Introduc Introduction tion to Engineeri Engineering ng calculati calculation: on: Variab Variables les,, their their dimens dimension ionss and units, units, dimens dimension ionally ally homoge homogeneo neous us and nonnon- homog homogene eneous ous equati equations ons,, standa standard rd condit condition ionss and ideal ideal gases, gases, physic physical al and chemical property data, basics of materials and energy balances in a macroscopic view point.

UNIT – II Fluid Mechanics: Principle of microbial nutrition, formulation of culture media, selective media, factors influe influenci ncing ng the choice choice of variou variouss carbon carbon and nitrog nitrogen en sources sources,, vitami vitamins, ns, mineral minerals, s, precur precursor sorss and antifoam agents, importance of pH, fluid vs. solids, fluid static’s mass and energy balance in fluid flow, Bernoulli’s equation, flow past immersed bodies and drag coefficient. Sterilization of process fluids, recovering and purifying products, integration of reaction and separation.

UNIT – III Heat Transfer: Principles and design of processes involving biochemical reactions, including aerobic and anaerobic respiration respiration and fermentation fermentation (involving (involving pure and mixed cultures). Shake flask, flask, batch and continuous operations. Solid state fermentations. Primary and secondary metabolites Energy balances and biochemical kinetics.

UNIT – IV Diffusion and Mass Transfer: Biological production consideration, large scale production, Enzyme kinetics, cell growth, energetics and mass transfer. Production of Penicillin, Streptomycin, Tetracycline and other Antibiotics. Text/ Reference Books:

1. Biopro Bioproces cesss Engine Engineerin ering, g, Second Second Editio Edition, n, Shuler Shuler ML; Kargi Kargi F (2002) (2002),, Prenti Prentice ce Hall PTR, PTR, New Jersey. 2. Biopro Bioproces cessin sing. g. Ward, Ward, O.P. O.P. (1991) (1991),, New York, York, 3. Bioseparati Bioseparationsons- Downstream Downstream processin processing g for biotechnolog biotechnology. y. Reinhold Reinhold Van Nostrand, Nostrand, Belter P.A, Cussler E.L, Hu W.S. (1988), John Wiley and Sons. New York.

29

4. Process Process Enginee Engineering ring in in Biotechno Biotechnology logy.. Jackson Jackson , A.T. 5. Biopro Bioproces cesss Engine Engineerin ering: g: System Systems, s, equipm equipment entss and facilities facilities.. Eds. Eds. Lyders Lydersen en K.B., K.B., D’elia, D’elia, N.A. and Nelson K.L. (1994), John Wiley & Sons, New York. 6. Chemical Chemical Engineering Engineering,, Vol.1-6, Coulson Coulson J.M, J.M, and Richardso Richardson n J.F (1999), (1999), Pergamon Pergamon Press. Press.

30

5th Semester B. Tech. (Bio - Technology) Downstream Processing BTT-307 E L 3

T 1

Theory : 100 Marks Sessional : 50 Marks Total

: 150 Marks

Time

: 3Hrs.


UNIT –I Introduction: History and scope of downstream processing in biotechnology, problems, requirement of purification. Overview of a bioprocess including upstream and downstream processing. Characteristics of biotechnology products, classes of bioproducts, physicochemical basis of bioseparation

UNIT – II Separatio tion n of partic particula ulate te by filtrat filtration ion,, centri centrifug fugatio ation, n, settli settling, ng, sedime sedimenta ntatio tion, n, Cell disintegr disintegratio ation: n: Separa decant decanting ing and micro micro filtrat filtration ion.. Primar Primary y isolat isolation ion method methodss includ including ing solven solventt extract extraction ion,, sorpti sorption, on, preci precipita pitatio tion, n, ultra ultra filtrat filtration ion and revers reversee osmos osmosis. is. Applic Applicati ation on of above above method methodss in purifi purificati cation on of antibiotics and enzymes. Purification methods: Fractional precipitation, electrophoresis, electro dialysis and various kinds of

chromatography.

UNIT – III Dynamicc immobi immobiliza lizatio tion, n, revers reversee osmosi osmosis, s, super super critica criticall fluid fluid Emerging Emerging separati separation on tech technique niques: s: Dynami extraction evaporation, super liquid extraction and foam based separation. Separation of intracellular, extracellular, heat and photosensitive materials. Product recovery trains - a few examples.

UNIT – IV Downstream processes and effluent treatment: applications of Unit Operations in Downstream with special reference to membrane separations & extractive fermentation, anaerobic and aerobic treatment of effluents. Typical examples for downstream processing and effluent disposal in process industries. Text/Reference Text/Reference Books:

1. 2.

Bioche Biochemic mical al Engin Engineeri eering ng funda fundamen mentals tals 2nd ed. Bailey Bailey J. J. E. and Ollis Ollis D. F. (198 (1986) 6) MacGraw MacGraw Hill, Hill, New York . Princi Principle pless of ferment fermentatio ation n technol technology ogy,, Stanbur Stanbury, y, P. F. F. and Whit Whitake aker, r, A. (1984 (1984), ), Pergam Pergamon on press. press.

31

3. 4. 5. 6. 7.

Unit Unit Operati Operation on of Chem Chemica icall Engine Engineerin ering g 6th ed. ed. McCabe, McCabe, W. W. L; Smith Smith J. J. C and Harriot Harriottt P. (2000 (2000). ). MacGraw Hill, New York Separat Separation ion Proce Process ss Princi Principle ples, s, Seader Seader,, J.D. & Henle Henley, y, E.J. E.J. (1998) (1998) John John Wile Wiley y & Sons, Sons, Oxford. Oxford. Biosep Bioseparat aration ion:: Downstr Downstream eam Proce Processi ssing ng for for Biotech Biotechnol nology ogy.. Belter, Belter, P. A.; Cussl Cussler er E. L. and and Hu W. S. (2003) John Wiley & Sons. OXFORD. Bios Biosep epara arati tion onss Scie Scienc ncee and and Engi Engine neeri ering ng,, Harri Harriso son n R.G. R.G.;; Todd Todd P.; Rudg Rudgee S.R. S.R. and Petrid Petrides es D.P. (2003). Oxford Press. Wastewa Wastewater ter Engi Enginee neerin ring g 4th 4th ed. ed. Metcalf Metcalf and and Eddy Eddy (2002) (2002).. MacGraw MacGraw Hill, Hill, New New York. York.

32

5th Semester B. Tech. (Bio - Technology)

Diagnostic Techniques Techniques BTT-309 E L 3

T 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT – I

Introduction: Comparison of the methods to diagnose bacterial & parasitic infections. Immunological Diagnostic Procedures: Procedures: Basic consider consideratio ations: ns: Antige Antigen-a n-anti ntibod body y reactio reactions. ns. Signal Signal amplif amplificat ication ion syste systems. ms. Isolat Isolation ion and characterization of antibodies. Immuno assay systems. Assay development, evaluation and validation. Reagent formulation and their shelf life evaluation.

UNIT-II Enzyme-Li Enzyme-Linked nked Immunoso Immunosorben rbentt Assay Assay (ELISA) (ELISA) system system: Applic Applicatio ations ns in clinic clinical al diagn diagnosi osiss and prognosis of various diseases. Membrane based Rapid I mmuno assays. Monoclonal Antibodies: Format Formation ion and select selection ion of hybrid hybrid cells. cells. Screen Screening ing for specif specific ic antibo antibodie diess producing hybrid cell lines. Detection of polypeptid polypeptidee hormones, hormones, Tumor markers markers and Applicati Applications ons of Monoclon Monoclonal al Antibodie Antibodies: s: Detection cytokines. Diagnosis of infectious diseases and drug monitoring. Detection of miscellaneous targets e.g. Thyroxin, Vit. B 12, Ferritin degradation products, Tau protein etc.

UNIT-III DNA Diagnostics- a)Nucleic acid hybridization assay systems: Basic considerations. Production of vari variou ouss type typess of hybr hybrid idiz izati ation on prob probes es.. Diag Diagno nosi siss of Plasmod Plasmodium ium falicip faliciparum, arum, Mycobact Mycobactrium rium Non - radioa radioact ctive ive Sickle le cell cell by DNA DNA hybrid hybridizat ization ion.. b) Non tubercul tuberculosus osus,, Trypanos Trypanosoma oma cruzi cruzi and Sick chromogenic or chemiluminesen chemiluminesentt substrates substrates and specific specific enzymes Hybridization procedures: Use of chromogenic Fingerprinting and RAPD as Diagnostic tools. for detecting signal amplification. DNA Fingerprinting UNIT-IV

Molecular diagnosis of Genetic of Genetic Diseases: Significance In prenatal diagnosis, diagnosis before onset of symptoms and identification of carriers of hereditary disorders. PCR/OLA Procedures: Diagnosis of hereditary diseases caused by mutations not affecting restriction endonuclease sites. Genotyping with fluorescence labeled PCR primers primers. Detection of mutations at different sites within one gene.

Text/Reference Text/Reference Books:

1. Essentials Essentials of Diagnosti Diagnosticc Microbiology. Microbiology. Shimeld Shimeld Lissa Lissa Anne and Rodger Rodgerss Anne T. (1998) (1998) Delmes Learning.

33

2. Reco Recomb mbin inan antt DNA DNA.. 2nd Edition. Watson James D and Gilman Michael, (2001). W.H Freeman and Company, New York. 3. Mole Molecu cula larr Biot Biotec echn hnol olog ogy: y: Princi Edition. Glick Principles ples Applicat Application ion of Recombin Recombinant ant DNA. 2nd Edition. Bernard R. and Pasternak Jack J. (1998), ASM press Washington DC. 4. Methodo Methodolog logy y of immuno immunoche chemica micall and immuno immuno-log -logica icall researc research h Kwapins Kwapinski ki G and Bannaty Bannatyne ne (1973) Willey inter science. 5. A handbo handbook ok of practi practical cal and clinical clinical immunolo immunology. gy. Talwar Talwar G.P, and Gupta S.K (1992), (1992), Vikas Vikas Publishing house Pvt. Ltd. New Delhi.

34

5th Semester B. Tech. (Bio - Technology) BIOSTATISTICS & COMPUTER APPLICATIONS BTT-311 E L 3

T 1



UNIT I Introduction : Define statistics. Difference between statistics and mathematics. Data in Biology: Samples and variables, frequency distributions, graphics. Basic quantitative methods .

UNIT II Confidence limits for means, means, t-distribut t-distribution, ion, chi-square chi-square distributio distribution. n. Estimation, hypothesis testing : Confidence Confidence limits for variances, t-tests, comparisons of variances. Comparisons of several means : Analysis of Variance, A priori tests, A posteriori tests. Two and three-way analyses of variance.

UNIT III Regression and correlation: Multiple Regression. Analysis of covariance. Nonlinear fitting. Correlation. Nonparametric Nonparametric statistics: statistics: Goodness of Fit tests. Resampling methods.

UNIT IV Probability distributions: Normal, Binomial and Poisson. Applications of Statistical Methods in Biotechnology. Biotechnology. Role of Computer in solving biostatistical problems . Text/Reference Text/Reference Books:

1. Introduction to Biostatistics. Glover T. and and Mitchell K. (2002). MacGraw Hill, New York. 2. Fundamentals of Biostatistics. Rosner Bernard. (1999), Duxbury Press.

5th Semester, B. Tech. (Bio - Technology) Recombinant DNA Technology Lab.

35

BTT- 313 E

L

T

P

-

-

4 Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs

Note: A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available

Cloning and expression of proteins: 1. Targ Target et sele select ctio ion n 2. Stra Strate tegy gy for for clon clonin ing g 3. Prim Primer er desig esign n 4. Isol Isolat atio ion n of geno genomi micc DNA DNA 5. Gene Gene amp ampli lifi ficat catio ion n by PCR PCR 6. Ligati Ligation on of of desir desired ed gene gene sequen sequence ce 7. Tran Transf sfor orma mati tion on 8. Veri Verific ficat atio ion n of clo clone ned d DNA DNA 9. Indu Induct ctio ion n of expr expres essi sion on 10. Verification Verification of protein expression expression

References: Molecular Cloning – A laboratory manual 3 rd Edition Vol. 1-3. Sambrook J. and Russell D.W. (2001) Cold Spring Harbor laboratory Press, New York.

36

5th Semester, B. Tech. (Bio - Technology) Fermentation and Downstream Processing Lab. BTT- 315 E L

T

P

-

-

4 Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs

Note: A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available

1. Study of factors affecting bioprocesses bioprocesses in submerged fermenters fermenters (pH, O2, Temperature, Foam, Ingredients) 2. Purify Purify a bacter bacterial ial protei protein n a) Cell Cell lysis lysis by differe different nt meth methods ods.. b) Cell debris separation separation by different different method methods. s. c) Colu Column mn puri purific ficat atio ion n I Separation by Molecular weight. II By charge. III By metal affinity. IV By Receptor-Ligand affinity. d) Dialysis e) Ultr Ultraf afil iltr trat atio ion n f) Cry Crystal stalli liza zati tio on g) Lyop Lyophi hili liza zati tion on 3. Puri Purifi fica cati tion on of OO-PS PS a) Cell lysis b) Harvesting of cells c) Purification of O-PS antigens References: 1. Bioprocess Bioprocess Enginee Engineering: ring: Systems, Systems, Equipm Equipment ent & facilities. facilities. Eds. Lydersen Lydersen K.B.; K.B.; D’elia D’elia N.A. and Nelson K.L. (1994) John Wiley & Sons, New York. 2. Bioprocess Bioprocess Techno Technology-K logy-Kinetic ineticss and Reactors. Reactors. Moser Moser Anton Anton (1988) (1988) Springer-Verlag , New York. 3. Bioseparati Bioseparationsons- Downstream Downstream processi processing ng for biotechnol biotechnology. ogy. Belter, Belter, P.A.; Cussler, Cussler, E.L. E.L. and Hu, W.S. (1988) John Wiley and Sons , New York. 4. Encycloped Encyclopedia ia of Bioprocess Bioprocess Technology: Technology: Fermentatio Fermentation, n, biocatalysis biocatalysis and bioseparat bioseparation ion Vol. 1-5. Eds. Flickinger M.C. and Drew S.W. (1999) John Wiley & Sons, New York. 5. Physic Physical al Biochem Biochemist istry: ry: Princ Principle iples s & applic applicati ations ons.. Sheehan Sheehan David David (2000) (2000) John John Wiley & Sons Ltd. New York. 6. Phys Physic ical al Bio Bioch chem emis istr try y 2nd Edition. Friefelder D. (1983) W.H. Freeman & Co., USA. 7. Biophy Biophysic sical al Chemist Chemistry: ry: Princi Principle ples s & techni technique ques s 2nd Edition. Upadhyay, A.; Upadhyay, K. and Nath, N. (2002) Himalaya Publication House, New Delhi.

37

5th Semester, B. Tech. (Bio - Technology) Diagnostic Techniques & Biostatistical Analysis Lab. BTT – 317 E L -

T -

P 3

Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs Note: A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available 1. Enzyme Enzyme Immuno Immunoass assay ay in Plat Plates es a. Conjug Conjugati ation on of antibo antibody dy with with enzyme enzyme.. b. OD vs. vs. Antige Antigen n conc. conc. plot plot and its its analys analysis. is. 2. Point Point of of Care Care –Membr –Membrane ane base based d assays assays a. Dips Dipsti tick ck bas based ed ass assay ayss b. b. Immu Immuno nofi filt ltra rati tion on c. Late Latera rall flo flow w assa assays ys 3. Immu mmunobl noblo ots 4. DNA primer primer probe probe selec selectio tion n a. Desig Design n pro probe bess for for dise diseas ases es b. Use commercial commercially ly available available probes probes to amplify amplify gene gene fragment fragment in clinical clinical samples samples.. c. Detec Detecti tion on of amp ampli lifi fied ed prod produc uctt 1. Gel Gel elect electro roph phor ores esis is 2. Dot Blots 3. Hybr Hybrid id captu capture re assa assays ys 5. Genotyping HLA 6. DNA DNA Fing Finger erpr prin inti ting ng 7. Biostatistical analysis of scientific data

References: 1. Antibo Antibodie dies: s: A laborato laboratory ry manual manual.. Harlow Harlow,, Ed and Lane, Lane, David David (1988) (1988) Cold Cold Spring Spring Harbo Harbor r laboratory Press. 2. Introductio Introduction n to Biostati Biostatistics: stics: Glover, Glover, T. and Mitchell, Mitchell, K. (2002) (2002) McGraw-Hill, McGraw-Hill, New York. York.

38

6th Semester B. Tech. (Bio - Technology) Microbial Biotechnology BTT-302 E L 3

T 1



UNIT I

Bioc Biocata ataly lysis sis and Enzym Enzyme e Biot Biotech echno nolo logy: gy: Biomimet Biomimetic ic cataly catalysis sis,, industrial industrial biocatalysis biocatalysis,, extremozymes extremozymes,, modular enzymes, enzymes, cofactor cofactor dependent enzymes and cofactor regeneration Isol Isolat atio ion n and and Puri Purifi fica cati tion on of Enzy Enzyme mes: s: Extr Extrac acti tion on of enzym enzymes, es, preparation of crude enzymes, purification of enzymes, processing of enzymes. .

UNIT II Protein and Enzyme Engineering: Basic principles, methods and their applications

Meta Metabo boli lic c Engi Engine neer erin ing: g: Heterologous gene expression: comp comple leme ment ntin ing, g, trans ransfferri erring ng and and engi engine neer erin ing g of meta metabo boli lic c pathways, redirecting metabolite flow.

UNIT III Single Cell protein (SCP): Introduction, conventional protein sources, substrates, microorganisms used, SCP from CO2 , carbohydrates, hydrocarbons. Molecular Breeding of Biosynthetic pathways: Metabolic engineering engineering for carotenoid, polyhydroxyalkanoates and alkaloid biosynthesis. Pathway analysis, metabolic control analysis, metabolomics.

39

UNIT IV Microbes and Microbial Genomics for Industry: Microbial transformations: transformation of steroids, sorbi sorbitol tol,, sorbos sorbosee and antib antibio iotic tics. s. Micr Microb obes es in pape paperr indu indust stry ry,, bioh biohyd ydrromet ometal allu lurrgy and and biomineralization. Microbial Genomics in industry: Analysis Analysis of microbial microbial genomes genomes and their use for designing designing vaccines and drugs. Text/Reference Text/Reference Books:

1) Biotechnol Biotechnology ogy and Genom Genomics. ics. Gupta, Gupta, P.K. (2004 (2004)) Rastogi Rastogi Publicatio Publications, ns, Meerut, Meerut, India. India. 2) Biotech Biotechnol nologi ogical cal Innova Innovatio tions ns in Chemic Chemical al Synth Synthesi esis. s. M.C.E M.C.E Van Dam–mier Dam–mieras as et al. (1997) (1997).. Butterworth-Heinemann, Oxford. 3) Biotechnol Biotechnology. ogy. Smith, Smith, J. J. E. (1996) (1996) Cambridge Cambridge Univer University sity Press. Press. nd 4) Methods Methods for for General General and Molecu Molecular lar Bacteri Bacteriolo ology gy 2 Edition. Gerhardt, P.; Murray, R.G.; Wood, W.A. & Kreig, N.R. (1994) Blackwell Publishing.

40

6th Semester B. Tech. (Bio - Technology) Plant Biotechnology BTT-304 E L 3

T 1

Theory : 100 Marks Sessional : 50 Marks Total

: 150 Marks

Time

: 3Hrs.

Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The Students Students will be required required to attempt attempt five questions, questions, selecting selecting at least one from each unit.

UNIT I Introduction: Cryo and organogenic differentiation. Types of culture: seed, embryo, callus, organ, cell and protoplast culture.

Micropropagation: Axillary Axillary bud bud proliferati proliferation, on, meristem meristem and shoot shoot tip cultur culture, e, bud culture culture,, organoge organogenesi nesis, s, embryoge embryogenesi nesis, s, advanta advantages ges and disadvantages of micropropagation. In Vitro itro haploid haploid product production ion:: Androgen Androgenic ic methods methods:: anther anther culture culture,, microspore culture, factors effecting and organogenesis. Signi Signifi fican cance ce and use use of haplo haploid ids, s, ploidy ploidy leve levell and chrom chromos osom ome e doub doubli ling ng,, dipl diplod odiz izat atio ion. n. Gy Gyno noge geni nic c hapl haploi oids ds:: fact factor ors s effe effect ctin ing g gynogenesis, gynogenesis, chromosome chromosome elimination elimination techniques techniques for production production of haploids in cereals. UNIT II

Proto Protopl plast ast Isol Isolati ation on and fusio fusion: n: Methods Methods of protopla protoplast st isolat isolation, ion, protop protoplast last develop developmen ment, t, somatic somatic hybridi hybridizat zation, ion, identif identifica icatio tion n and selection of hybrid cells, cybrids, potential of somatic hybridization, limitations. Somaclonal variation: Nomenclature, methods, applications basis and disadvantages. Gametoclonal variation. nitrogenase, hydrogenase, nodulation, Growth Plant Growth Promoting bacteria: Nitrogen fixation, nitrogenase, promotion by free-living bacteria.

UNIT III

41

Plant Plant Molecul Molecular ar Biolog Biology: y: Plant Plant gene gene struc structu ture re as a disc discon onti tinuo nuous us gene, control sequences Transient ent and stable stable gene gene expres expressio sion, n, marker marker genes, genes, select selectabl ablee marker markers, s, Gene transfer transfer in plants: plants: Transi chimeric gene vectors. transposable elements. Vectorless or direct direct DNA Gene transfer methods: Agrobacterium, viruses and transposable transfer: Physical, chemical and imbibation methods of gene transfer.

UNIT IV

Transg Tr ansgeni enics cs in crop improve improvement ment:: Resist Resistanc ance e to biotic biotic stress stressesesinse insect ct,, viru virus s and and dise diseas ase e (fun (fungu gus s and and bact bacter eriu ium) m) resi resist stan ance ce,, herb herbic icid ide e resi resist stan ance ce.. Deve Develo lopm pmen entt of stre stress ss and and sene senesc scen ence ce-toler olera ance nce – Oxidat idatiive stress, salt stress ess and and fruit uit ripeni penin ng. Transg ransgeni enics cs for : improv improved ed quali quality ty,, longer longer life life,, flow flower er color color and shapes, for male sterility, for terminator seed. Trangenic plants as bior biorea eact ctor ors: s: prod produc ucti tion on of carb carboh ohyd ydra rate tes, s, lipi lipids ds,, vita vitami mins ns and and miner minerals als,, biode biodegra gradab dable le plas plasti tics cs,, pepti peptides des,, prote proteins ins and edibl edible e vaccines. Commercial transgenic crops. Text/Reference Text/Reference Books:

1. Intr Introd oduc ucti tion on to Plan Plantt Biot Biotech echno nolo logy gy 2 nd edition. Chawla, H.S. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi 2. Molecu Molecular lar Biotechn Biotechnolo ology: gy: Principl Principles es and Applicat Application ionss of recombina recombinant nt DNA. Glick, B. R. and Pasternak J. J. (1998) ASM press, Washington DC. 3. Plant Plant Tissue Tissue culture culture:: Theory Theory and Practic Practice. e. Bhojwa Bhojwani, ni, S.S. S.S. and. Razda Razdan n M.K (1996) (1996) Elsevie Elsevier r Science, Netherlands. 4. Improving Improving Plant Plant draught, draught, salt and freezing freezing tolerance tolerance by gene transfer transfer of a single single stress-indu stress-inducible cible transcription factor. (1999) Nature Biotechnology 17(3): 287-291. Kasuga, M., Q. Liu, et al. 5. Hete Hetero rolo logo gous us expr expres essi sion on of Arabidopsis phy phyto toch chro rome me B in tran transg sgen enic ic pota potato to infl influe uenc nces es photosynthetic performance and tuber development.(1999) Physiology 120, (1):73-81. Thiele, A., Herold M., et al. 6. Buildi Building ng better better trees trees with with antisen antisense. se. (1999 (1999)) Nature Biotechnology 17 (8): 750-751. Sederoff R. 7. Exploiting the full potential of disease-resistance genes for agricultural use. Curr Opin Biotechnol. 2000 Apr;11(2):120-5. Review Rommens CM, Kishiore GM 8. Directed molecular evolution in plant improvement. Curr Opin Plant Biol. 2001 Apr;4(2):152-6. Review. Lassner M, Bedbrook J.

42

6th Semester B. Tech. (Bio -Technology) Animal & Aquaculture Biotechnology

BTT-306 E L 3

T 1



UNIT I Introduction: History and scope of animal biotechnology. Basic techniques techniques of animal cell culture & their application applications. s. Balanced Balanced salt solutions solutions and simple simple growth growth media. Serum quality and cell culture. Cryoprese eserva rvatio tion n and transpo transport rt of animal animal Preser Preservat vation ion and mainte maintenan nance ce of animal animal cell cell lines: lines: Cryopr germplasm (i.e. semen, ovum and embryos).

UNIT II Transgen Transgenic ic animals animals Methodol Methodology ogy: Retr Retrov ovira irall vect vector or meth method od,, DNA DNA micro microin inje jecti ction on meth method od and and engine engineered ered embryo embryonic nic stem stem cell cell method method.. Clonin Cloning g by nuclear nuclear transfe transfer. r. Yeast Yeast artific artificial ial chromo chromosom somee transgenesis.

In Vitro fertilization and embryo transfer.

UNIT III Molecu Molecular lar biolog biologica icall techni technique ques s for rapid rapid diagno diagnosis sis of geneti genetic c disea diseases ses and gene gene therap therapy. y. Molecular maps of animal genomes. Chemical carcinogenesis. Transfection. Oncogenes and antioncogenes. Gene cloning techniques for mammalian mammalian cells, establishment of immortal cell lines, cloning in mammal mammalian ian cells, cells, expre expressi ssion on of mamma mammalia lian n genes genes in prokar prokaryot yotic ic and eukary eukaryoti otic c syste systems ms.. Extinction of gene function by antisense RNA and DNA.

UNIT IV Aquaculture: Introduction. Water resources and types of culture systems (Fish & Prawn). General principles of nutrition. Engineering considerations. Food, bait and ornamental marine species. Transgenic fishes. Text/Reference Text/Reference Books:

1. Prin Princi cipl ples es of Gene Gene Manipu Manipula lati tion onss 6 th edition. Primrose S.B.; Twyman, R. and Old B. (2002) Blackwell Publishing.

43

2. Molecular Molecular Biotech Biotechnolo nology: gy: Principles Principles and Application Applicationss of recombinant recombinant DNA 2 nd Edition. Glick, B. R. and Pasternak J. J. (1998) ASM press, Washington DC. 3. Animal Animal Cell biotechnol biotechnology ogy : Spier, R.E. and Griffiths Griffiths J.B. (1988) (1988) Academic Academic press. press. 4. Living Living resour resources ces for Biotechnol Biotechnology, ogy, Animal cells. Doyle, Doyle, A.; Hay, R. and Kirsop, Kirsop, B.E. (1990) (1990) Cambridge University Press, Cambridge. 5. Animal Animal Biotech Biotechnolo nology. gy. Murray Murray Moo-Young Moo-Young (1989) (1989) Pergamon Pergamon Press, Press, Oxford. Oxford. 6. Introductio Introduction n of Aquaculture Aquaculture Landau Landau Matthew Matthew (1991) (1991) John Wiley Wiley & Sons, Sons, New York. York.

6th Semester B. Tech. (Bio - Technology)

Healthcare Biotechnology BTT-308 E L 3

T 1



UNIT I

Simple proteins and therapeutic agents: Proteins as therapeutic agent gents s, choice of express ession systems ems and and opt optimi imizing ing gene ene expr expres essi sion on.. Appl Applic icat atio ions ns,, deli delive very ry and and targ target etin ing g of ther therap apeu euti tic c pro protteins eins.. Engi Engine neer erin ing g huma human n int interf erferon erons s and and huma human n grow growth th hormones. Regulatory aspects of therapeutic proteins. Enzyme Enzymes s as therap therapeut eutic ic agent agents: s: Use Use of genet genetic ical ally ly engin enginee eered red DNase I and alginate lyase for treatment of Cystic Fibrosis UNIT II

Monoclon Monoclonal al Antibodies Antibodies as Ther Therapeut apeutic ic agents: agents: Produc Productio tion n of Monocl Monoclona onall antibo antibodie dies. s. Human Human Monoclonal antibodies – its scope and a nd limitations. Hybrid human - mouse antibodies. Production of antibodies in E. coli. Approaches for producing HIV therapeutic agents.

44

UNIT III Human Diseases and Vaccines: Viral & bacterial diseases. Diseases caused by protozoan and parasitic parasitic worms (helminth (helminths). s). Emerging Emerging infectious infectious diseases. diseases. Active and passive passive immunity. Autoimmunity. Autoimmunity. Rational of immunization. Diseases controllable by vaccination. Vaccines: Designing Designing vaccines vaccines adjuvants. adjuvants. Whole organisms organisms vaccines-attenuate vaccines-attenuated d viruses viruses and bacteria. bacteria. Inactivation of pathogenic organisms by heat and chemical treatment.

UNIT IV Purified macromolecules as vaccines: Bacterial polysaccharides , proteins and toxins as vaccines. Recombinant vaccines: Subunit, attenuated and vector vaccines. Multivalent vaccines Vaccine development against AIDS. Commercial and regulatory aspects of vaccine production and its distribution. Text/Reference Text/Reference Books:

1. Molecular Molecular Biotechno Biotechnology: logy: Princip Principles les Applicati Application on of Recombin Recombinant ant DNA. 2nd Edition Edition Glick, B. R. and Pasternak, J. J. (1998) ASM press, Washington DC. 2. Basi Basicc Bio Biote tech chno nolo logy gy 2nd Ed. Ratledge, C. and Kristiansen, B. (2001) Cambridge University press. 3. New generatio generation n vaccines. vaccines. Woodsaw, Woodsaw, G.C. & Leine, Leine, H. M. Marcel Dekker Dekker Inc., Inc., New New York. 4. Recomb Recombina inant nt DNA vaccine vaccines: s: Rational Rationalee & Strateg Strategies ies.. Richar Richard, d, E.I. (1992) (1992) Marcel Marcel Dekker Dekker Inc., New York.

6th Semester B. Tech. (Bio - Technology) Environmental Biotechnology BTT-310 E L 3

T 1



UNIT I Role of Biotechnology in Environment Protection: Introduction and current status of biotechnology in environment protection and its future prospects.

Biologica icall treatme treatment, nt, impact impact of Microb Microbiol iology ogy and Bioch Biochemi emistr stry y of Waste Waste Water Water Tre Treatm atment ent:: Biolog pollutatnts on biotreatment, cell physiology and important microorganisms, plasmid borne metabolic activities, bioaugmentation, packaged microorganisms, use of genetically engineered organisms.

UNIT II

45

Bioreactors for Waste Water Treatment: Biological processes for industrial effluent treatment, aerobic biological treatment, anaerobic biological treatment, periodic biological reactors, membrane bioreactors, use of immobilized enzymes and microbial cells. Removal of Specific Pollutants: Sources Sources of heavy metal pollution, pollution, microbial microbial systems systems for heavy metal accumulation, biosorption, bioleaching.

UNIT III What is bior biorem emed edia iati tion on?? Type Typess of bior biorem emed edia iati tion on,, bioa bioaug ugme ment ntat atio ion n Biorem Bioremedi ediat ation ion : What bioremediation. Bioreactors for remediation processes. Applications of bioremediation.

for for

Biotechnology for Hazardous Waste Management : Xenobiotic compounds, recalcitrance, hazardous wastes wastes,, biodeg biodegrad radati ation on of xenobi xenobioti otics, cs, biolog biological ical detoxi detoxifica ficatio tion, n, biotec biotechno hnolog logical ical manage managemen mentt of hazardous wastes.

UNIT IV Rest Restor orat atio ion n of degr degrad aded ed land landss : Restor Restoratio ation n throug through h microo microorga rganis nisms, ms, Casuar Casuarina inass for tropic tropical al reforestation on adverse sites, development of stress tolerant plants, use of mycorrhizae in reforestation. Organic farming and use of microbes for improving soil fertility, reforestation of lands contaminated with heavy metals. Biotechnology for Waste Treatment of Food and Allied Industries: Biological treatment, methods, SCP and biomass from waste and distillery industry. Novel Methods for Pollution Control : Vermitechnology, waste water treatment using aquatic plants, root zone treatment. Aiming for biodegradable and ecofriendly products. Text/Reference Text/Reference Books: 1. Waste water water Engineering Engineering Treatmen Treatment, t, Disposal Disposal and Reuse. Reuse. Metcalf Metcalf & Eddy (1991) (1991) McGraw McGraw Hill. 2. Environmen Environmental tal Biotechnol Biotechnology. ogy. Forster, Forster, C. F and. and. Wase, D. A. J. (1987) (1987) Ellis Ellis Horwood Horwood Halsted Halsted Press. Press. 3. New Processes Processes of Waste Waste water treatmen treatmentt and recovery. recovery. G. Mattock Mattock E.D. E.D. (1978) (1978) Ellis Horwood. Horwood. 4. Biochemical Biochemical Engineer Engineering ing Fundamenta Fundamentals ls 2nd ed. ed. Bailey, Bailey, J. E. and Ollis, Ollis, D. F. (1986) (1986) MacGraw MacGraw Hill. New New York. 5. Environmental Biotechnology. Jogdand, S.N. (1995) Himalaya Publishing House, New Delhi. 6. Comprehens Comprehensive ive Biotechno Biotechnology logy (Vol. (Vol. 1-4) Young Young Murray Murray Moo (Ed.) (Ed.) (1985) (1985) Elsever Elsever Sciences. Sciences.

7. Stan Standa dard rd Method Method for Examin Examinat atio ion n of water water & wast wastee wate waterr 14th Ed. (1985) American Public Health Ass.

46

6th Semester B. Tech. (Bio - Technology) Food Biotechnology BTT-312 E L 3

T 1


Note for paper setter : Question paper will consist of four units. Eight questions will be set in the question paper by selecting two from each unit. The students will be required to attempt five questions, selecting at least one from each unit. UNIT I Introduction: Microorganisms in food – historical developments. Food Fermentation Technology: Origin, Origin, scope and developmen developmentt of fermented fermented products, products, primary feed stock, raw materials and conversions, fermented food and microbial starters, commercial potential, food fermentation industries, their magnitude, R&D innovations.

UNIT II Development of Novel Food and food Ingredients: Single cell protein, polysaccharides, low calorie sweeteners, naturally produced flavor modifiers, amino acids, vitamins, food supplements, food coloring, neutraceuticals, water binding agents. Bioreactors in Food Fermentations: Cultivation of microorganisms, instrumentation regulation and process control, laboratory scale submerged and solid state fermentation, pilot scale submerged and solid state fermentation.

UNIT III Food Spoilage Spoilage and Preserv Preservatio ation: n: General General principle principle of spoilage, spoilage, microbial toxins (endotoxins (endotoxins and exotoxins), contamination and preservation, factors affecting spoilage. Methods of food preservation (thermal processing, cold preservation, chemical preservatives & food dehydration). Radiation and Food Preservation: Preservation: Role of radiation in food preservation, characteristics of radiation of interest interest in food preservation. preservation. Principles underlying underlying the destruction destruction of microorgan microorganisms isms by irradiation. irradiation. Effect of irradiation on food constituents. Legal status of food irradiation.

UNIT IV Biological controls and Monitoring of food quality. Packaging of Food: Need for packaging, requirements for packaging, containers for packaging (glass, metal, plastics, molded pulp and a nd aluminium foil), dispensing devices. Text/Reference Text/Reference Books:

1. Mode Modern rn Food Food Mic Micro robi biol olog ogy y 6 th Ed. Jay, J.M. (2000). Kluwer Academic/Plenum Pub. 2. Food Food Microbio Microbiolog logy: y: Fundam Fundament entals als and Fron Frontie tierr 2 nd Eds. Ed. Beuchat, Doyle & Montville. (2001). Blackwell Synergy. 3. Food Microbio Microbiology. logy. Frazier, Frazier, W.C. W.C. and Westhoff, Westhoff, D.C. D.C. (1988) (1988) Tata Mc-Graw Mc-Graw Hill, New New Delhi.

47

4. Biotechnol Biotechnology. ogy. Gupta, Gupta, P.K. P.K. (1998) (1998) Rastogi Rastogi Publicatio Publication, n, Meerut. Meerut. India 5. Biotechnol Biotechnology: ogy: Food Food Fermentation Fermentation Vol. I & II. Eds. Joshi, Joshi, V.K. & Pandey, Pandey, A. (1999) (1999) Educational Educational Publishers and Distributers, Kerala. 6. Biotechnol Biotechnological ogical Strategies Strategies in Agropr Agroprocess ocessing ing Eds. Eds. Marwaha Marwaha S.S & Arora, J.K. (2003 (2003)) Asiatech Asiatech Publishers, New Delhi.

48

6th Semester, B. Tech. (Bio - Technology) Cell and Tissue Culture Lab BTT- 314 E L -

T -

P 4

Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs Note: A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available. A) Animal Cell Culture

1) Primary cell culture from 12-14 days mouse embryos. 2) Culture of fibroblast and epithelial cells. 3) Visualization of proteins by immunofluorescence. 4) Induction of interferon in cell culture. 5) Culture of human lymphocytes - PHA stimulation-estimation by amount of DNA. - Karyotyping. B) Plant Tissue Culture

1) Plant cell culture from different types of explants. 2) Isolation of DNA/RNA from cultured cells and compare with seeds. 3) Callus development for somatic embryogenesis. 4) Agrobacterium mediated transformation.

References: 1. Culture of Animal Cells – a manual of basic techniques 4 th Edition. Freshney, R. I. (2000) John Wiley & Sons , New York. 2. Animal Cell Biotechnology. Spier, R. E. and Griffiths, J. B. (1988) Academic Press. 3. Living resources for biotechnology: biotechnology: Animal Cells. Doyle, A.; Hay, R. and Kirsop, Kirsop, B. E. (1990) Cambridge University Press. 4. Plant Tissue Culture: Theory & Practice. Bhojwani, S. S. and Rajdan, M. K. (1996). Elsevier Amsterdam. 5. Experiments in Plant Tissue Culture. Dodde, J. H. and Robert, L. W. (1998).

49

6th Semester, B. Tech. (Bio - Technology) Healthcare Biotechnology Lab. BTT- 316 E L -

T -

P 4

Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs Note: A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available. 1. Normal microflo microflora ra of mouth and and determinatio determination n of its susceptib susceptibility ility to dental dental caries. caries. 2.

Preliminary Preliminary identificatio identification n of enteric enteric bacteria bacteria by TSI agar. agar.

3. Estimation Estimation of of ketone ketone bodies bodies in the the urine of normal normal persons persons.. 4. Fasting Fasting and post-prand post-prandial ial determin determination ation of blood blood glucos glucose. e. 5. Lipid profile profile (Total (Total cholesterol cholesterol,, TG, VLDL, VLDL, LDL and HDL) HDL) of human human blood sample samples. s. 6. Deter Determi minat natio ion n of Rh Rh fact factor or.. 7. VDRL VDRL test test for for syp syphi hili lis. s. 8. WIDAL WIDAL test test for for Salm Salmone onella lla/Ty /Typho phoid. id. 9. Preg Pregna nanc ncy y test test.. 10. Cultural sensitivity of pathogens against against various antibiotics. antibiotics.

50

6th Semester, B. Tech. (Bio - Technology) Microbial, Food and Environmental Biotechnology Lab. BTT- 318 E L -

T -

P 3

Practical/V.V.: 50 Marks Sessional: 50 Marks Total : 100 Marks Time : 4 Hrs Note : A college should offer 70% of the below listed experiments. The remaining 30% experiments may be modified by college according to facilities available. A. Microbial Biotechnology: 1. Production Production of of organic organic acids (Citric (Citric and lactic) lactic) by microor microorganis ganisms. ms. 2. Production Production of antibiotics antibiotics by microorgan microorganisms. isms. 3. Production Production of industria industrially lly important important enzymes enzymes (protease, (protease, amylase, amylase, cellulose, cellulose, xylase or lipase) lipase) by microorganisms. 4. Demonstrati Demonstration on of of bacterial bacterial lysis by bactriophages bactriophages..

B. Food Biotechnology: Microbiological analysis (bacterial count ) of food products. Isolation of bacteriocin bacteriocin producing microorganisms from fermented foods and determination of the antimicrobial spectrum of bacteriocin producing isolates. isolates. Cultivation of white button mushroom ( Agaricus biosporus ). Determination of food toxins. C. Environmental Biotechnology: Qualitative analysis of water/waste water: Bacterial analysis Determination of hardness, alkalinity, conductivity, chlorides, temperature and pH. Determination of soluble phosphates. Determination of BOD, COD and DO contents. Biobleaching/ Decolourization of industrial effluents/industrially important dyes by microbes. Microbial degradation of organic wastes eg: Lignocelluloses/ pesticides/ hydrocarbons. Vermicomposting.

51

Bachelor of Technology ( Bio-Technology) Scheme of Courses/examination Sr. No.

1 2 3 4

5 6 6 7



L

T

P/D P/D

Th. Th. Sess Sess.. P/VV P/VV TO TOTA TAL L

Bio-informatics Stem Cell in health care Essentials of Virology Inte Intell llec ectu tual al Prop Proper erty ty Rights in Biotechnology Products * Elective – I BTT-409E Bio-informatics Lab. BTT-411E Tr Training Report

4 4 3 4

1 1 1 1

-

5 5 4 4

100 100 100 100

50 50 50 50

-

150 150 150 150

3 3 3 3

3 -

1 -

3 -

4 3 -

100

50 50 100

50

150 100 100

3 3 -

BTT-413E Minor Project TOTAL

18 18

3

10 13

10 35

500

15 1 50 150 550 200

300 12 1250

3

Course No.

Subject

BTT-401E BTT-403E BTT-405E BTTBTT-40 407E 7E

TOTA TO TAL L

Duration of Exam.

Bachelor of Technology ( Bio-Technology) Scheme of Courses/examination Sr.No.

Course

Subject



Duration

No.

of Exam. L

T

1 1

-

5 5

100 100

50 50

-

150 150

3 3

0

-

3

-

50 50

50

100

3

1 -

3

5 3

100 -

50 50

50

150 100

3 3

6 6

BTT-402E Bioethics & Bio-safety 4 BTTBTT-40 404E 4E Mark Market etin ing g Man Manag agem emen entt 4 of Biotech Products BTTBTT-40 406E 6E Comm Commun unic icat atio ion n Ski Skill llss & 3 Professional Practice Elective – II 4 BTTBTT-40 408E 8E Adva Advanc nced ed Tech Techni niqu ques es in Biotechnology Lab. BTT-410E Seminar 2 BTT-412E General Proficiency -

-

-

2 -

-

50 50 100

-

50 100

-

7

BTT-414E Major Project

3

12 15

12 35

300

250 350

450 1250

-

1 2 3 4 5

Elective – I 1. BTT-415E 2. BTT-417E 3. BTT-419E 4. BTT-421E Elective – II 1. BTTBTT-41 416E 6E 2. BTT-418E 3. BTT-420E 4. BTT-42 -422E 5. BTT-424E

TOTAL 17

P/D P/D

TOTA TO TAL L

Th. Th.

Bio-Sensors and Bio-Instrumentation Biochips an and Microarray Te Technology Nanotechnology Pharmaceutical Bi Biotechnology Adva Advanc nced ed Mana Manage geme ment nt Info Inform rmat atio ion n Syst System emss Biocatalysts an and Bi Biotransformation Behavioural Neuroscience Herbal Drug Technology Human Genetics and Human Genome

Students have to choose one elective from each group

Sess Sess.. P/VV P/VV TO TOTA TAL L

200 600

B. Tech. Biotech Syllabus MDU

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