Prelab for Ch 22.2 Organic Chemistry LabFull description
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How to model natural gas dehydration using teg using aspen hysysFull description
Introduction to Hydrate Inhibition and Dehydration
DEHYDRATION
Jenivie Morillo Jose University of Santo Tomas National Children’s Hospital
process of removing EXTRACELLULAR and
INTRACELLULAR water from tissue
IDEAL CHARACTERISTICS: dehydrate tissue rapidly without SHRINKAGE
not EVAPORATE very fast able to DEHYDRATE even FATTY tissues not HARDEN the tissue excessively not be TOXIC to the handler
THREE COMMONLY SOLUTIONS: alcohol, acetone, dioxane ALCOHOL METHOD -use of ETHYL ALCOHOL, METHYL ALCOHOL, BUTYL and ISOPROPYL ALCOHOL are recommended for routine dehydration of tissues -BUTYL and ISOPROPYL ALCOHOL is SLOW DEHYDRATING AGENT -consists of passing the tissue through a series of progressively more concentrated alcohol baths -the more DELICATE the tissue, the LOWER is the grade of alcohol and SMALLER the intervals should be between the strengths of the ascending alcohols
ACETONE METHOD -for MOST urgent biopsies -UNSUITABLE for routine work
DIOXANE METHOD (Diethylene Dioxide) -UNIQUE reagent(miscible with both water and
molten paraffin wax) -should be used only in A WELL VENTILATED LABORATORY -EXCELLENT DEHYDRATING and CLEARING AGENT
TETRAHYDROFURAN -used as BOTH DEHYDRATING and CLEARING
AGENT (miscible in both water and paraffin) -dissolve FATS -miscible in LOWER ALCOHOLS, ETHER, CHLOROFORM, ACETONE, BENZENE and XYLENE -doesn’t dissolve out ANILINE DYES -MOST STAINING procedure gives improved results
CELLOSOLVE(Ethylene Glycol Monoethyether) -with RAPID ACTION without OVERHARDENING
and DISTORTION of TISSUES
Tri-ethyl Phosphate -removes water very rapidly -produces very little DISTORTION -soluble in ALCOHOL, ETHER, BENZENE,
CHOLOFORM, ACETONE and XYLENE -produces MINIMUM tissue shrinkage