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WEAO 2012 Conference. Anaerobic digestion is a well understood biosolids treatment technology. The primary benefits are the production of renewable energy and the reduction of material to be…Full description
A. Digestion in the Mouth What are the optimum conditions for salivary amylase?
Does salivary amylase continue to act in the stomach? In the intestine?
Name the substrate and the end product of salivary amyase activity.
4. Give other methods that may be used to determine amylase activity.
5. Correlate the gradual change in color response of the hydrolysis products to I 2 with their secondary structure.
Figure1. Iodine inside amylose resulting in a blue-black color .
6. Cite experimental evidences obtained in this experiment that illustrate the high efficiency of enzymes.
B. Digestion in the Stomach 1. What are the optimum conditions for the acivity of pepsin? Rennin?
. 2. Differentiate infant curdling from adult curdling.
3. Why is there no digestion of proteins in gastric juice with pH above 7?
4. What substances other than hydrochloric acid contribute to the total acidity of gastric contents?
C. Digestion in the Small Intestines 1. What are the end products of the action of pancreatic amylase on starch?
2. Why was hardboiled egg white and not raw egg white used in this experiment?
3. What is the role of CaCl2 in the action of pancreatic lipase?
LITERATURE CITED Ash, M. 2011. The role of HCl in gastric function and health. Retrieved from http://www.nleducation.co.uk/resources/reviews/the-role-of-hcl-in-gastric-function-and-health/
Czerbska, M.T. 2007. Psychoendocrinology research trends. USA: Nova Publishers. Douma, M., curator. 2008. Cause of http://www.webexhibits.org/causesofcolor.
Color.
Retrieved
November
26,
2014
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Food and Agriculture Organization. n.d. Digestion, absorption and energy value of carbohydrates. Retrieved from http://www.fao.org/docrep Keeton, W. 2014. In Encyclopedia Brittanica. Retrieved http://www.britannica.com/EBchecked/topic/1081754/human-digestive-system/242914/Gastricsecretion
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Leung,P.S. 2014. The Gastrointestinal System.: Gastrointestinal, Nutritional and Hepatobiliary Physiology. P.139. Springer Science & Business. Miller, S. 1992. Enzyme experiments. Retrieved from http://www.ableweb.org/volumes/vol-6/10miller.pdf Raugel, P. 1999. Rapid food analysis and hygiene montoring: Kites, instruments and systems. USA: Springer Science and Business Media. Schonheyder, F., Volqvartz, K. 2008. On the activation of pancreatic lipase by calcium chloride at varying pH. Retrived from http://onlinelibrary.wiley.com/doi/10.1111/j.17481716.1945.tb00291.x/abstract Sharma, J.P., n.d. Understanding Biology. India: Goswami Printers. Sigma Aldrich Corporation. 2014. Enzymes Data. Retrieved from http://www.sigmaaldrich.com/lifescience/metabolomics/enzyme-explorer/analytical-enzymes/pepsin.html#sthash.HV5khRpT.dpuf Talwar, G.P and Srivastava, L.M. 2002. Textbook of Biochemistry and Human Biology. p.337. PHI Learning Pvt.Ltd. Tortora, G.J. and Derrickson, B.H. 2008. Principles of Anatomy and Physiology. P.954. John Wiley & Sons.
