Lab Report 1 Biology Photosynthesis

Sarthak Patel Biol 1108 Section 001 2/23/15 Lab Report 1: Photosynthesis Lab Effect of  on  an! Introduction:

Photosynthesis an! cell"lar respiration are i#portant bioche#ical life processes that in$ol$e a series of catalytic reactions% Photoa"totrophs are able to con$ert of inor&anic carbon !io'i!e to or&anic( hi&h)ener&y &l"cose "sin& li&ht ener&y in their chloroplasts *EEB !ept% 201+,% -n co#parison cell"lar respiration happens in the cytoplas# of bacteria an! in the #itochon!ria of e"karyotic cells .here hi&h ener&y &l"cose is broken !o.n in or!er to capt"re ener&y as P #olec"les% his e'peri#ent .ill seek to e'a#ine rate of p hotosynthesis an! respiration in a spinach leaf tiss"e "sin& leaf !isks% e pre!ict that "n!er li&ht con!itions( the leaf !iscs .ill !isplay  photosynthesis an! "n!er !ark con!itions the leaf !iscs .ill sho. cell"lar respiration% respiration% -n a!!ition( .e hypothesie that if the li&htin& apparat"s is #o$e! farther a.ay fro# the leaf !iscs then the rate of photosynthesis .ill be obser$ably obser$abl y slo.er% ))

 .hat is biolo&ical reason



Photosynthesis Li&ht Ener&y

462 7 426 ⇒ 41264 7 462

ell"lar Respiration 41264 7 462 ⇒ 462 7 426 7 P

Materials and Methods:

Spinach lea$es .ere p"nche! o"t in or!er to create leaf !iscs that .ere initially place! in a syrin&e barrel .ith the pl"n&er re#o$e!% he pl"n&er .as replace! an! slo.ly p"she! to.ar!s the tip in or!er to re#o$e air after .hich 3 cc of 0%29 bicarbonate/!eter&ent b icarbonate/!eter&ent sol"tion .as a!!e! to the syrin&e as a 62 so"rce for the leaf !iscs%  $ac""# .as create! insi!e the syrin&e an! the !iscs .ere allo.e! to sink an! then po"re! into a plastic c"p containin& bicarbonate sol"tion% he c"p .ith the !iscs .as place! "n!er a li&ht so"rce an! the n"#ber of !isks floatin& at the s"rface .as recor!e! e$ery #in"te "ntil all !iscs are floatin&% s photosynthesis occ"rs in the  presence of 62 an! li&ht( o'y&en .ill be pro!"ce! an! !iscs .ill e'pan! an! rise% fter all !iscs ha$e risen the c"p .ill be place! in a !ark cabinet( cab inet( an! the rate at .hich the leaf !iscs sink .ill  be "se! to #eas"re cell"lar respiration% -n the control e'peri#ent( the plastic c"p consiste! of !istille! .ater in or!er to represent the absence of 62 an! the rate of photosynthesis .as #eas"re!% -n a!!ition( a secon! e'peri#ent .as perfor#e! .here the li&htin& apparat"s .as hei&htene! an! place! farther a.ay fro# the leaf !iscs an! the effect e ffect on the rate of  photosynthesis .as analye!%

PSET50:

Figure 1. 6bser$e! floatin& !iscs o"t of 15 in the presence an! absence of li&ht%

Figure 2. 6bser$e! floatin& !iscs o"t of 1 in !istille! .ater an! a hei&htene! li&ht so"rce%

;o!"le 1: he bosrpiton Spectra of Plant Pi&#ents ;o!"le 3:
=isc"ssion:

References: nee! 2 =epart#ent of Ecolo&y an! E$ol"tionary Biolo&y( >ni$ersity of onnectic"t% Biolo&y 1108 Laboratory ;an"al( 201+ e!ition( >ni$ersity of onnectic"t( Storrs( onnectic"t%