Must Know in Mycology

Must know in Mycology                            

Basic structure of fungi – hyphae and spore General culture media – SDA Czapek’s medium – Aspergillus Serum at 37OC – germ tube Cornmeal agar – Chlamydospore Media with olive oil – Malassezia furfur Hair baiting test – T. mentagrophytes Trichophyton – skin, hair, nails Red color, tear drop conidia – T. rubrum Favic chandelier (antler) – T. schoenleinii Coenocytic – Phycomycetes / Zygomycetes Ascospore – Saccharomyces cerevisiae C. neoformans – Niger birdseed (+), urease (+), phenol oxidase (+), India ink Arthrospore – Geotrichum, Coccidioides, Trichosporon, Aureobasidium Blastospores – yeast 10% KOH – direct microscopic, clearing LPCB – common fungal stain Wright / Giemsa – H. capsulatum Dichotomous hyphae – Aspergillus Thrush, diaper rash, Moniliasis – Candida Actinomyces – Sulfur granules Nocardia – modified acid fast Macro- microconida – Dermatophytes Sputum – systemic mycoses CHO assimilation – ID yeast, free of CHO BHIA- dimorphic fungi C. immitis – high risk to lab personnel Chlorampenicol and cycloheximide – inhibitory agent for mycosel / mycobiotic