MICROBIOLOGY – MYCOLOGY LAB 1 – OPPORTUNISTIC MYCOSES USTMED ’07 Sec C – AsM; pictures provided by JV.N.
SYNCEPHALASTRUM Gray to brown to black fluffy colony that may fill a Petri dish in a few days. Similar to Mucor and Rhizopus spp.
NEUROSPORA (MONILIA) White at first but grows rapidly filling the entire Petri dish in a few days and becoming a salmon to brown color. Mycelium may hang from the lid of the Petri dish.
Broad, clear, nonseptate hyphae, spores in many slender sac-like structures (sporangia) adhere to a swelling on the terminal end of hypha.
Clear septate hyphae with large masses of ovate spores which are airdispersed
CEPHALOSPORIUM
HORMODENDRUM
White to tan to rose-colroed colony, eventually developing White aerial hyphae.
Green to gray to black colony on both sides. Often wrinkled and grows flat
Single celled, clear, elliptical spores held together in a ball unless broken loose
Dark brown septate hyphae bear branching chains of elongate to ovate spores that often contain a small black dot at the end. Spore bearing structures look tree-like
NIGROSPORA Rapid growing, producing abundant fluffly, aerial hyphae, gray to black on both sides. Resembles Mucor or Rhizopus
SCOPULARIOPSIS
Powdery, light brown, wrinkled colony resembling Penicillium spp. Except for color. Large, clearly visible jet black spores.
Resembles Penicillium spp. except spores are larger and form unbranched-chains CHAETOMIUM Slow growing, flat, white, yellow, tan or brown colony PAECILOMYCES Flat, rapid-growing, tan-colored colony resembling Aspergillus spp.
Large, dark, central structure is perithecium that contains ascospores (sexually produced)
Similar to Penicillium spp. except small spores are produced on very long, slender structures
BOTRYTIS
Flat, compact colonies, white at first then becoming black, green, bluish or yellow
Soft looking tan to gray colony
Small one-celled spores irradiating out from swollen base (see arrows) Colorless, one celled spores borne in clumps
PENICILLIUM White colony at first but developing blue to green color
STEMPHYLUM Brown to black, wrinkled fuzzy colony
Small, round spores borne in “brush-like” formations Hyphae are brown and strongly septate: huge multicelled terminal spores that may either be smooth or rough
MUCOR (?)
SEPEDONIUM
Cottony, rapid grower. May completely fill a Petri dish in 3 to 5 days. Brown to gray.
Fluffy, white colony resembles Histoplasma capsulatum
Clear, nonseptate hyphae. Spores borne inside large spherical structures called sporangia. Similar to Rhizopus spp. but lacking rhizoids.
Large, rough-walled spores that resembles Histoplasma capsulatum
RHIZOPUS GLIOCLADIUM
Gray to brown to black colony filling a Petri dish in 2 to 3 days. Similar to Mucor spp. Mucor spp. Flat, rapid-growing colony. White at first, then developing dark green central portion
Numerous small spores held together in a clump. Similar to Penicillium spp. Except for the clumping of spores.
ASPERGILLUM (?)
Similar to Mucor spp. Mucor spp. except foot-like structures (rhizoids) at base of spore bearing hyphae (see arrows). Spores sporangium clear, coenocytic hyphae
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FUSARIUM Fast-growing colony. At first, white and cottony but develping rose to red color on both sides
TRICHODERMA SPECIES
Largest spores are sickle-shaped and may contain several cells. Small spores with one to two cells have more rounded ends.
Classification: Contaminants Mounting fluid used: Lactophenol Cotton Blue (LPCB)
TRITRACHIUM SPECIES
GEOTRICHIUM White to tan, flat or fluffy, rapidgrowing fungus
Classification: Contaminants Mounting fluid used: LPCB
DRESCHLERA SPECIES Note hyphae breaking into arthrospores. May be confused with Coccidioides immitis.
ALTERNARIA
Classification: Contaminants Mounting fluid used: LPCB
Rapid-growing colonies, grayish to black to brown; underside jet black
Large, hand grenade-shaped spores with both longitudinal and transverse cross walls. Borne singly or in chains. Septate, dematiaceous fungi.
* MALASSEZIA FURFUR
KOH Smear – Skin scrapings Positive for Short hyphal elements with oval bodies (Malassezia furfur)
TEASE MOUNT TECHNIQUE
CURVALARIA
Dark brown to black colony on both sides ragged in appearance.
For the tease mount, a fragment of the colony colony collected using a wire or loop is transferred to a glass microscope slide. A drop of lactophenol lactophenol cotton blue is then adde added, d, and and the the spec specim imen en is teased using dissecting dissecting needles as show shown n in this this figu figure re.. The teasing of the specimen needs to be done done care carefu full lly. y. Isol Isolat ated ed elements can be observed, while at the same time preserving the integrity of the over-all structure of the microorganism.
ADHESIVE TAPE TECHNIQUE Large, bent spores with 3 to 5 cells. Similar to Helminthosporium spp. Brown, septate hyphae.
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A p ie iec e of t ra ra ns ns pa pa re re nt nt adhe adhesi sive ve tape tape is used used to coll collec ectt the the spec specim imen en by pressi pressing ng the adhesi adhesive ve slide slide agai agains nstt the the surfa surface ce of the the fungal colony. Aerial elements will adhere to the tape, which is subseq subsequen uently tly placed on a microscopic slide containing a drop drop of lactop lactophen henol ol cotton cotton blue. This technique is good for preserving the original relationship between spores and aerial hyphae. However, it usually cannot be applied to mold specimens that have few
aerial mycelia or to yeast with a moist consistency.
