There are two points as the aims of this experiment: (1) To identify protein by using peptide bond at protein through biuret test, sulfur test, precipitate by metal, precipitate by salt and protein...Full description
Exercise 10 (Amines, Amino Acids and Proteins)Full description
Characterized proteins and amino acids through different tests namely Biuret Test, Ninhydrin Test, Hopkins-Cole Test, Lead Acetate Test, Sakaguchi Test and Xanthoproteic Test.Full description
The test bank from the publisher for the 4th edition ofFull description
The test bank from the publisher for the 4th edition ofFull description
The test bank from the publisher for the 4th edition ofDescripción completa
Crystals, X-rays and Proteins Comprehensive Protein Crystallography by Dennis Sherwood
Banking occupies one of the most important positions in the modern economic world. It is necessary for trade and industry. Hence it is one of the great agencies of commerce. Modern banking services presence is very helpful to the economic activity an
BSPH 2 AGROUP2
: Ms . Mel ani eA. Cor puz, RPh I ns t r uc t or
Memb mber s :
Dat e:a J nuar y15,2016
Aqui no ,Ang e l i c a
Subj e c t : Bi o c he mi mi s t r y( La bo r a t or y)
Ba s i l i o, J a me me s Due n a s , S a r a h Sa r mi mi e nt o,J us t i ne Suya t , Chr i s t i ne S ant i a go, Ka t e Exper i mentNo.9 PROP OPERTI ESOFPROT OTEI NS Que s t i o nsf o rRe s e a r c h : 1 . Ex pl a i nt hea c t i o no fpr o t e i na sabuffe r . Pr ot ei nscanac tasbuffer sbecauseeachpr ot ei nmol ec ul ei sbot haweakac i danda
we a kb a s e .Thewe we a k l ya c i di cc a r bo x yl i ca c i dsc o unt e r a c tr i s i ngpH whi l et hewe we a k l y ba s i c ami no gr oupsc an c ount e r ac tf al l i ng pH.Pl a s ma pr ot e i nsand he mogl obi n ( bl o od' so xy ge nc a r r yi ngpi gme nt ,apr o t e i n)e nha nc et heb l o od' sbuffe r i ngc a pa c i t y . ( Pr o t e i nsa l s ohe l pwi t hbuffe r i ngwi t hi nc e l l s . ) 2 . Ac c o untf o rt hee ffe c t i v i t yof7 0 %a l c o ho la sdi s i nf e c t a ntc o mpa mp r e dt ot hea c t i o no f 95% al c ohol . 7 0 % pe r c e n tofa l c o holi si de a lt oas t r o ng e rs o l ut i o n.Pur ea l c o ho l( 9 5 1 0 0 %a l c o ho l )
c o a gul a t e spr o t e i ni nc o nt a c t .S uppo s et hepur ea l c o ho li spo ur e do ve ras i ngl ec e l l e d or g ani s m.Thea m. l c o ho lwi l lg ot hr ought hec e l lwa l lo ft heo r g a ni s mi na l ldi r e c t i o n, c o agul a t i ngt hepr o t e i nj us ti ns i det hec e l lwa l l .Ther i ngoft hec o agul a t e d pr o t e i n woul dt hen s t op t heal coholf r om penet r at i ng f ar t herf r om t hece l l ,and no mor e c o a gul a t i o nwo ul dt a kepl a c e . Att hi st i met hec e l lwo ul dbe c o mei me na c t i v ebutno tde a d. Unde rt hef a v or a bl ec ondi t i o nst hec e l lwoul dt he nbe gi nt of unc t i o n.I f7 0pe r c e n to f a l c o holi spour e dt oas i ngl ec e l l e dor ga ni s m,t m, hedi l ut e da l c o hola l s oc o agul a t e st he pr o t e i n,buta tas l o we we rr a t e ,s ot ha ti tp ene t r a t e sal lt hewa yt hr ought hec e l lbe f or e c o agul a t i onc a nbl oc ki t .The nt hee nt i r ec e l li sc o a gul a t e da ndt heor ga ni s m di e s . 3.H . How dot hef ol l owi wi ngs ubs t anc esa c tasdenat ur i ngagentf orpr ot ei ns ? Hea vyMe Met al s Hea vymet al sac tt odenat ur epr ot ei nsi nmuc ht hes ame mema mannerasac i dsandbas es . Heavymet als al t susual l ycont ai nHg+2,Pb+2,Ag+1Tl +1andot hermet al swi t hhi gh
a t omi cwe i ght s .Si nc es a l t sa r ei oni ct he ydi s r upts a l tbr i dge si npr o t e i ns .Ther e a c t i on ofahe a vyme t a ls a l twi t hapr o t e i nus ua l l yl e a dst oa ni ns o l ubl eme t a lpr o t e i ns a l t . Al ka l oi da lr e a ge nt s Al ka l oi dr e a ge nt s ,s uc ha st a nni ca c i d( us e di nt a nni ngl e a t he r )t he s er e a ge nt sc ombi ne wi t hpos i t i v e l yc h ar g e da mi nogr o upsi npr o t e i nst odi s r upti o ni cb onds . I nor ga ni ca c i ds De na t ur a t i o ni sc ha r a c t e r i z e db ynuc l e i ca c i dsorpr o t e i nsl o s i ngt he i rs e c o nda r ya nd t e r t i a r ys t r uc t ur eduet oa ne x t e r na lc o mpound o rs t r e s sa ppl i c a t i o n.I no r g a ni ca c i d i nt e r f e r e swi t ht hea mi noa c i dbo ndst ha tma keupapr o t e i n' st e r t i a r ys t r uc t ur e .Thi s c ha ng e st hepr o t e i n' ss ha pea spa r toft hede na t ur a t i onpr oc e s s . 4 . Whyi si ti mpo r t a ntt ha tt hepH v a l ue so fi t sflui dsb ec o nt r o l l e di na no r g a ni s m? OurbodypH atvar i ousl evel sofourbody’ sflui dor gani zat i oni sver yi mpor t ant be c aus epH c ont r ol st hes pe e dofourbody' sbi oc he mi c alr e ac t i ons .I tdoe st hi sby c o nt r ol l i ngt hes pe e do fe nz ymea c t i vi t ya swe l la st hes pe e dt ha te l e c t r i c i t ymo ve s t hr ough
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