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Gravitizer to The Rescue The feasibility of Aqueous Extract of Guyabano ( Anona Muricata L.) Peelings as a main comonent in ma!ing a han" sanitizer
Esma#a$ %asmine %. Roxas$ Arianne &ae P.
'ubmitte" in artial fulfillment of the requirements in Research
aloocan *igh 'chool +,th Avenue Grace Par!$ aloocan ity August ++- $ ,+/
&rs. 0iola 1. 2onato Research A"viser
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Table of Contents
Preliminary Parts
Title Page
1
Table of ontents
Abstract De"i#ation
! $
C%a&ter I' Intro"(#tion
1ac!groun" of the 'tu"y
)
'tatement of the Problem
*
'ignificance of the 'tu"y
*
'coe an" 2elimitation of the 'tu"y C%a&ter II
Revie: of Relate" ;iterature
+
oncetual 3rame:or!
1, 2
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*yothesis
1,
2efinition of Terms
11
C%a&ter III' Met%o"olo-y
1
C%a&ter I.' Res(lts an" Dis#(ssion
'tatistical Analysis
1!
C%a&ter ./ Con#l(sion0 Re#ommen"ation
*
onclusion
1)
Recommen"ation
1)
C%a&ter .I
1ibliograhy Ac!no:le"gements
1* 1+
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Abstra#t
&icrobes srea" easily in our community by means of having hysical contact :ith a contaminate" ob house. The ingre"ients use" :ere urchase" from "ifferent rivate sellers. n ro"ucing the natural han" sanitizer$ Gravitizer$ the guyabano eelings :ere obtaine" an" :ashe". The guyabano eelings :ere blen"e" to obtain the eeling>s aqueous extract :ith less effort. The aqueous extract of the eelings hol"s an anti=microbial roerty an" vitamins :hich can imrove our immunity. The Aloe 0era Gel :as mixe" to the extract to avoi" several s!in comlications. &ostly$ guyabano eelings are thro:n a:ay it :ill be much economical if :e are able to create something out of it. Almost everybo"y can ro"uce an" buy one. 1y the use of this natural sanitizer :e can imrove our health an" revent microbes from harming our :ell=being. As :e all !no:$ revention is better than cure.
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De"i#ation
The researchers are "e"icating this research to Go"$ ?ur 3ather$ for he>s the one :ho ma"e this research ossible an" for giving us strength to continue this :or!. The researchers are also "e"icating this research to their Research A"viser$ &rs. 0iola 1. 2onato$ :ho ha" been giving a"vices that hele" the researchers accomlish every challenge they met "uring the exeriments.
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Intro"(#tion
&icrobes are every:here. They are naturally occurring in the soil$ air$ :ater$ human bo"y$ animals$ insects an" other non=living ob
Ba#-ro(n" of t%e St("y
n or"er for us to live$ :e must maintain a goo" health. The emergence of "iseases is a threat for being ro"uctive. A man "oes his activities remar!ably :hen he is healthy. An" in or"er to "o so$ :e must ta!e revention. The researchers aim to ro"uce an antimicrobial solution that :ill hel era"icate certain bacteria that can hel microbes from harming our society>s :ell=being. The aqueous extract of the Guyabano fruit has an antibacterial roerty. t is antimicrobial against 'tahyllococcus aureus$ a Gram ositive cocci :hich is a natural
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flora of the human bo"y esecially the face$ an" 0ivrio cholerae :hich is a ositive agent of cholera an" causes "iarrhea that can lea" to "ehy"ration$ infection an" comlication. Antimicrobial or bacterici"al substance "estroys bacteria. 1ase" from @i!ie"ia$ han" sanitizer is an alternative for :ashing our han"s :ith soa an" :ater. t can be reare" in form of gel$ foam or liqui" solution. The use of an alcohol base" han" sanitizer is more effective in !illing microbes than soas. t can revent the transmission of "angerous "iseases. Statement of t%e Problem
s sourso or guyabano an essential ingre"ient to ro"uce an effective han" sanitizer Si-nifi#an#e of t%e St("y
?btaining certain illness an" "iseases from microbes can cause health comlications or even "eath. These cases can be also revente" by the use of a han" sanitizer. The guyabano aqueous extract a""e" to the han" sanitizer have antimicrobial roerties that can "estroy certain bacteria that other commercialize" han" sanitizers can>t.
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Re2ie3 of Relate" 4iterat(re
This chater is a revie: of the existing literature relevant to the toic of Guyabano>s otential. &ore secially$ this chater is a revie: of toics inclu"ingB :hat can guyabano contains$ the effect of its extract. n this art of research$ are relate" i"eas an" toics about guyabano an" ho: can it affect our lives. The follo:ing information are gathere" from "ifferent literatures$ both local an" foreign. 4o#al 4iterat(re
Guyabano (Anona &uricata)$ or sourso in English$ is a small troical fruit tree bearing a s:eet$ heart hae" fruit :ith a soft=sine green outer s!in an" soft uly :hite flesh. The fruit :as teste" against stahylococcus$ e coli an" other common bacterias for anti=microbial caability an" toxicity. (&alaya$ ,+) The fruit together :ith its leaves$ stems$ roots$ see"s an" bar! is concocte" into exensive
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harmacoeia$ consi"ere" the bible of harmacologyC. t is :i"ely use" in cosmetics an" toiletries for it is reorte" moisturizing an" revitalizing action. There are also claims of the beneficial an" even curative roerties of aloe vera gel in the self=treatment of me"ical con"itions (&irror @ee!ly$ +---). Anona muricata have exhibite" antibacterial activity against numerous microorganisms such as Plasmo"ium falciarum$ Escherichia coli$ Pseu"omonas aeruginosa$
'higella
flexneri$
'tahylococcus
aureus$
'tahylococcus
albus$
'almonella tyhosa$ 'almonella ne:ort$ an" 'erratia macescens (Raintree 4utrition$ nc. ,,7). The guyabano fruit is an excellent source of 0itamins 1 an" though the flesh of this fruit :as reorte" to be "eficient in calcium$ hoshorous an" 0itamin A. (Duisumbing$ +-9+).
Forei-n 4iterat(re
4umerous stu"ies have been con"ucte" on the lant Anona muricata ;. 2ue to its :ealth of cytotoxic cancer substances active against numerous cancer cell lines$ this Guyabano fruit ha" been given more attention than the others. (Reiser et al. +--/). Anona muricata ;. is !no:n to have antimicrobial roerties. The Guyabano have antibacterial$ antifungal$ an" antiviral otentials. (Taylor ,,9).
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Con#e&t(al Frame3or
8(yabano Fr(it
E7tra#tion of 8(yabano A9(eo(s E7tra#t
8ra2iti:er ;8(yabano 5an" Saniti:er<
5y&ot%esis 6 O&erational
There is a significant "ifference bet:een guyabano sanitizer an" commercialize" sanitizer. 6 N(ll
There is no significant "ifference bet:een guyabano sanitizer an" commercialize" sanitizer.
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Definition of terms
8(yabano = Guyabano fruit is a green$ ear=shae" fruit covere" :ith soft sines.
&ature" guyabano or sourso fruit :eighs about to 9 !ilograms. Guyabano fruit is ovoi" an" large$ u to + centimeters long$ :ith thin s!in an" the soft e"ible :hitish ul that is fleshy an" fibrous. Guyabano has ine"ible blac! see"s. Guyabano fruit or sourso fruit has "istinct s:eet=sour flavor that tastes li!e ineale an"
stra:berry :ith a tang of sour citrus taste. Aloe .era = Aloe vera is a cactus lant that belongs to the ;iliaceae family. t gro:s in "ry climates such as those foun" in arts of Africa an" n"ia an" has been use" me"icinally for centuries. Aloe leaves secrete a clear gel that :hen bro!en off from
the rest of the lant that can be alie" toically to heal :oun"s an" soothe s!in. 5an" Saniti:er = is a sulement or alternative to han" :ashing :ith soa an" :ater. 0arious rearations are available$ inclu"ing gel$ foam$ an" liqui" solutions. The active ingre"ient in han" sanitizers may be isoroanol$ ethanol$ n=roanol$ or ovi"one=io"ine
Met%o"olo-y 11
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8at%erin- of t%e Materials
3resh guyabano fruits :eighing 5!g :ere gaine" from a ublic mar!et near the researcher>s house. The 9,, m; Aloe 0era gel :as obtaine" from a rivate seller in &untinlua ity. 1o:l$ la"le$ an" han" sanitizer container :ere use" in the exeriment$ all of :hich are obtaine" from the researcher>s house. E7tra#tion of t%e 8(yabano A9(eo(s E7tra#t
After the searation of the eelings from the fruit$ the researchers gaine" less than half a !ilo of eelings. The eelings :ere blen"e" by the use of an electric mixer. The eelings :ere lace" on a cloth an" :as comresse" manually. 9m; of aqueous extract from the eelings :as obtaine" Mi7in- t%e In-re"ients
&ixing of ingre"ients in the bo:l one by one :as "one. The 9m; aqueous extract gaine" :as oure" into the bo:l an" :as follo:e" by 69m; Aloe vera gel. t :as stirre" an" the solution :as then transferre" to a clean han" sanitizer container.
E7&erimental Desi-n 12
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The researchers lanne" to have +, ran"om testers for the han" sanitizers. The testers :ill aly both of the han" sanitizers but in "ifferent han"$ for examle$ the Gravitizer :ill be alie" in the left art :hile the commercialize" in the right art. After testing both han" sanitizers$ the researchers let the testers rate the han" sanitizers from +=9. The "ata collecte" is sho:n belo:. Tester
Commer#iali:e"
8ra2iti:er
A
!
*
B
)
1,
C
>
>
D
)
>
E
*
>
F
*
)
8
$
>
5
!
+
I
*
>
?
+
1,
4e-en"' -=+, F Excellent 6= F 0ery Goo" 9=5 F Goo" /=7 F 3air += F Poor
Res(lts an" Dis#(ssion Statisti#al Analysis Table
?ne of the most common statistical analyses$ the T=test for T:o n"een"ent 'amles$ is use" to evaluate the mean "ifference bet:een t:o oulations using the "ata from t:o seerate samles. The general urose of the in"een"ent measures t
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test is to "etermine :hether the samle mean "ifference obtaine" is a real "ifference bet:een the t:o oulations or simly the result of samling error.
Tester
Commer#iali:e
8ra2iti:er
Table 9.
" <
<
The
A
!
=,/*
,/!+
@
=1
1
calculate"
B
)
=,/*
,/!+
$
1
1
t is
C
>
,/*
,/!+
!
,
,
@ith a
D
)
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,/!+
$
,
,
E
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,
,
F
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!
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1
8
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5
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,
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of ,.9$ t
I
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must
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+
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,/!+
$
1
1
Hx
)@
.69+.
"egree of free"om of + an"
excee" or
>
be equal to )/@
>/
'tan"ar" 2eviation
1/)@)!
1/++@
0ariance
/)**>
1/$111
2.101.
Accor"ing to the
results$ t calcttabular . The comutate" t=score of .69+ excee"s the tabular value of t$ then 14
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the researchers conclu"e that there is a statistically significant robability that the relationshi bet:een t:o variables exists an" not "ue to chance$ an" re
Con#l(sion
1ase" on the "ata gathere"$ the researchers therefore conclu"e that the gravitizer is more effective than the commercialize" han" sanitizer in terms of texture$ smell an" moisturizing effect. The testers also commente" the goo" effects that they felt "uring the testing.
Re#ommen"ation
The researchers recommen" to not only to use the gravitizer but to exan" its natural :on"er. 'o that in the near future this stu"y :ill be further more enhance an" so as the ro"uct :hich is the gravitizer.
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Bibilio-ra&%y
= +st entury Iniversal Encycloe"ia8 0ol.+7 F *av=*y"8 'y"ney8 %& 2ent An" 'ons ;t" Publishing +-/8 +-= Grolier nternational8 2anbury$ onnecticut8 +-- = 4e: 'tan"ar" Encycloe"ia8 0ol. +5 F 'ma='z8 'tan"ar" E"ucation ororation8 hicago8 +--5 = Encycloe"ia Americana8 0ol. 9 = omton>s Encycloe"ia8 0ol. +,8 +--98 hicago8 Printe" in I'A8 omton>s ;earning omany8 Tribune Publishing omany = Encycloe"ia 1rittanica8 oc!er$ 2ais8 'cotlan"8 +65 = Grolier nternational$ 0ol. +9
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= GuyabanoB Puly$ s:eet an" healthy8&alaya84ovember /$ ,+8.A+ =Guyabano for cancer 8 Rafael astillo8 Philiine 2aily nquirer8 &ay 9$ ,+8 .19 = Duestions raise" over use" of Aloe 0era8 &irror @ee!ly8 ?ctober 9$ +---8 .+ = Guyabano=Duezon ityB 2AJn"K= +.B+++.hotos8+cm8 2eartment of Agriculture = httBLLchemistry.about.comLo"LcleanerchemistryLaLhan"=sanitizer=recie.html = httsBLLen.:i!ie"ia.orgL:i!iLGlycerol = httBLL:::.:i!iho:.comL&a!e=Mour=?:n=@aterless=*an"='anitizer = httBLLen.:i!ie"ia.orgL:i!iLAloeNvera = httBLL:::.mommyotamus.comLho:=to=ma!e=non=toxic=han"=sanitizer = httBLLvoices.yahoo.comL/=easy=han"=sanitizers=home=+++6/.html
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A#no3le"-ement
The researchers :oul" li!e to give their gratitu"e to their arents for suorting the researchers financially$ emotionally an" intellectually. 3or encouraging the researchers to accomlish this research. 3urthermore$ the researchers :oul" also li!e to than! Rozel 4i!!o ;ea#o$ &T$ :ho rovi"e" certain !no:le"ge about the toic being stu"ie" by the researchers. &ost of all$ the researchers :oul" li!e to than! Go"$ ?ur 3ather$ for giving us the strength an" insiration to continue this research until the en". The researchers :oul" also li!e to give their gratitu"e to &rs. 0iola 1. 2onato$ Research A"viser$ :ho gui"e" the researchers an" gave sufficient !no:le"ge for them to accomlish this research that :oul"nOt have been ossible :ithout her assistance.
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Esma#a$ %asmine %. Roxas$ Arianne &ae P. The Researchers
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