Yeast Lpac

Readthepatent Benzaldehyde(106mg,1mmol), petroleumether(80ml), ethanol(0.5ml), baker'syeast(5g) andthepyruvicacidbuffer(5ml) werestirredat50°C.for24h. Thatisalittleover90mltotalsofigure`100mlandthatgetsyoujust30mg L-PAC Times3333=333liters.Usea350Lstirredbioreactorregulatedto50C.That getsyou100gifyoudoeverythingright. OnceUponaTimethefamousUncleFesterrecommendednextproportionofstarters forbrewingL-PAC: 1partofglucose,1partofyeast,20partsofwater,1/12partofBenzaldehyde . If(asUncleFesterclaimed)youcouldgetof85%yieldofL-PAConyourBenzald ehyde, Sotoget100gr.ofL-PAC,youprobablywillneed120gr.ofBenzaldehyde,1.44 kg.ofsugar,1.44kg.yeast, 28.8L.ofwater.Consideringthefoam,you'llprobablyneedvolumeof50-60lit recontainer.