BIOCHEMISTRY(AIPG) 5. 2013 no questions
Gout is disorder of A. Purine metabolism B.
Pyrimidine metabolism
C.
Ketone metabolism
D. Protein metabolism Ref: Harrison 17/e p. 2444
2010 2012 1.
What is the most sensitive indicator of cellular 32. Most LIPOGENIC carbohydrate
energy? A. ATP
A. Fructose
B.
ADP
B.
Glucose
C.
Phosphocreatine
C.
Ribose
D. AMP
D. Sucrose Ref: Harper 22/e p 193 2011
33. Denaturation of DNA A. Fragmentation of DNA molecules
1.
Which of the following is not present in basal lamina
B.
Reversible separation of DNA
A. Rhodopsin
C.
Irreversible separation of DNA
B.
Entactin
D. Coagulation of DNA
C.
Laminin
D. Integrin
Ref: Aynes 2/e p. 427 34. Cereals are deficient in
Ref: James 3/e p. 294 2.
A. Vitamin C B.
Vitamin B – Complex
Shingomyelinse deficiency is seen in
C.
Iron
A. Niemann pick disease
D. Calcium
B.
Garber disease
C.
Tay sach’s disease
Ref: Lippincott 2/e 15
D. Krabbe’s disease 35. ATP are store house of? Ref: Satyanarayan 3/E, 307 3.
A. Energy B.
Phosphate
Decreased BMR is seen in
C.
Protein
A. Obesity
D. Glucose
B.
Hyperthyroidism
C.
Feeding
Harper 22/e 337
D. Exercise 36. During Ref: Ganong 22/e p. 462
phagocytes
respiratory
activation of A. Oxidases
4.
Somatomedin mediate
B.
Hydrolase
A. Chondritin sulfate deposition
C.
Peroxidase
B.
Lipolysis
D. All
C.
Gluconeogenesis
D. Decreased rate of glucose uptake by cells 37. FSH, LH, TSH acts through Ref: Ganong 22/e p. 382
A. Cyclic AMP
burst
involves
B.
Cyclic GMP
C.
Both
D. Transcription activators
D. None
Ref: Harper 26/e, p. 458
Harper 22/e 427
44. Insulin causes lipogenesis by all EXCEPT? A. Increasing acetyl CoA carboxylase activity
38. The Highest percentages of polyunsaturated fatty
B.
Increase the transport of glucose into the cell
acids are present in?
C.
Inhibit pyruvate dehydrogenase
A. Ground nut oil
D. Decrease intracellular cAMP level
B.
Palm oil
C.
Margarine
D. Soyabean oil
45. True about G – protein coupled receptors is A. G protein bind to hormones on the cell surface
Park 19/e 481
B.
All the three subunits alpha, beta & Gamma should bind to each other for G protein to act
39. ___ is not a transcription factors
C.
A. Insulin
G proteins acts as inhibitory and excitatory because of difference in alpha sub unit
B.
Estrogen
C.
Vitamin D
D. G protein bound to GTP in resting stage
D. Glucocoticoid
Ref: Harper 26/e, p. 458
40. Ist clinical sign of vitamin A deficiency is
46. Thiamine
deficiency
causes
decreased
energy
A. Night blindness
production because
B.
Bitot’s spots
A. It is required for the process of transamination
C.
Xerostomia
B.
It is co – factor in exudative reduction
C.
It is co
D. Conjunctival dryness
- enzyme for transketolase in pentose
phosphate pathway D. It is co – enzyme for pyruvate dehydrogenase
Davidson 19/e 318 41. The reducing equivalent for fatty acid synthesis is
Ref: Harper 26/e, p. 488
derived from 47. All are true about glutathione EXCEPT
A. Glycolysis B.
Kreb’s cycle
A. It is a tripeptide
C.
Urea cycle
B.
It converts Hb to met Hb
C.
It conjugates xenobiotics
D. Pentose phosphate pathway
D. It scavenges free radicals and superoxide ions Harper 22/e 202 42. Compared
to
cow’s
Ref: Harper 26/e, p. 629 milk,
mother’s
milk
has
48. In molecular cloning Blue – White screening is used
abundance of
for
A. Lactose
A. To screen for recombinant vectors
B.
Fat
B.
To detect gene mutation
C.
Protein
C.
To identify desired chromosomal DNA insert in plasmid vectors
D. All
D. To detect host DNA in Situ Park 19/e 500 2009 43. Steroid hormone receptors have attachment site for all
2008 49. Amino acid which is used in transportation of
EXCEPT
ammonia and helps in urea synthesis is
A. Steroid hormone
A. Arginine
B.
Transcription repressors
B.
Glutamate
C.
Hormone responsive element
C.
Aspirtate
D. Glycin Ref: Lippincott’s 2/e, p. 286, 240-241
Ref: Guyton 8/e p. 870-81 56. A small Ca2+ binding protein that modifies the activity of many enzymes and other proteins in response of
50. Which of the following enzyme is stable at acidic pH?
changes of Ca2+ concentration is known as
A. Pepsin
A. Cycline
B.
Trypsin
B.
Calmodulin
C.
Chemotrypsim
C.
Collagen
D. Carboxypeptidase
D. Kinesin
Ref: Harper27 / e p. 485 51. Type of collage found in hyaline cartilage is
Ref: Harper 27/E p. 482 57. Which of the following has low glycemic index?
A. Type I
A. Whole wheat bread
B.
Type II
B.
Brown rice
C.
Type III
C.
Ice cream
D. Type IV
D. Potato
Ref: Harper 27/E p. 561, 546 52. Protein purification and separation can be done by all
Ref: Harper 27/E p. 482 58. Keton bodies are utilized by all EXCEPT
EXCEPT
A. RBC
A. Centrifugation
B.
Brain
B.
Electrophoresis
C.
Heart
C.
Densitometry
D. Muscles
D. Chromatography Ref: Harper 27/E p. 191-192 Ref: Harper 27/E p. 21-24 59. Which of the following is a good sources of saturated fatty acids 53. All of the following processes are involved in the
A. Palm oil
detection of mRNA expression EXCEPT
B.
Sunflower oil
A. Northern blot
C.
Ground nut oil
B.
RT – PCR
D. Olive oil
C.
Microarray
D. RNA sequencing Ref: Harper 27/E p. 409-410
Ref: lippincott’s 2/e, p 314 60. All are good sources of n – e poly – unsaturated fatty acids EXCEPT
54. All of the following are the components of basement
A. Groundnut oil
membrane EXCEPT
B.
Mustard oil
A. Rhodopsin
C.
Corn oil
B.
Laminin
D. Fish oil
C.
Entactin
Ref: Harper 27/E p. 123
D. Dystroglycan 61. Which of the following maskes hydrophobic to Ref: Harper 27/E p. 550-571
hydrophilic? A. Methylation
55. Which of the following is the most active form of
B.
Acetylation
calcium in the circulation?
C.
Glucuronidation
A. Ionized calcium
D. Hydroxylation
B.
Complexed with phosphate
C.
Complexed with oxalates
D. Bound to albumin
Ref: Harper 27/E p. 633-637
62. The conversion of ethanol to aldehyde is mediated by A. Alcohol dehydrogenase B.
Aldehyde dehydrogenase
C.
Xenthine oxidase
D. Catalase Ref: Harper 27/E p. 225, 95,96
2007 68. The most abundant protein present in body is A. Globulin B.
Albumin
C.
Collagen
D. Hemoglobin 63. Which of the following participates in transamination
Ref: Harper 27/E p 88
reaction? A. Thiamine pyrophosphate
69. All of the following types of collagen EXCEPT one are
B.
Pyridoxal phosphate
present in cartilage
C.
Cyanocobalamine
A. 2
D. Niacin Ref: Harper 27/E p. 247
B.
4
C.
6
D. 9
64. Regarding pentose phosphate pathway all of the
Ref: Harper 27/E p 561
following are true EXCEPT A. Occurs in the cytosol
70. All of the following are sarcolemmal proteins EXCEPT
B.
No ATP is produced in the cycle
A. Syntrophin
C.
It is active in adipose tissue, liver, and gonads
B.
Dystrophin
C.
Dystrobrevin
D. The oxidative phase generates NADPH and the nonoxidative phase generates pyruvate
D. Dysmorphin
Ref: Harper 27/E p. 177, 180
Ref: Harper 27/E p 575 71. High content of cholesterol is present in
65. All of the following are precursor of adrenaline
A. Coconut oil
EXCEPT
B.
Egg yolk
A. Noradrenalin
C.
Hydrogenated fat
B.
Phenylalanine
D. Ghee (unsaturated)
C.
Isoprenaline
D. Dopamine Ref: Harper 27/E p. 276, 455
Ref: Harper 27/E p. 230 72. Fatty acids help in synthesis of all EXCEPT A. Glucose
66. Intrinsic factor
B.
Cholesterol
A. Is a polysaccharide
C.
Ketone bodies
B.
Is a glycoprotein
D. Fat
C.
Helps in foliate absorption
D. Helps in blood coagulation Ref: Harper 27/E p 499-500
Ref: Harper 27/E p. 134 73. Increase in pyruvate and lactate is seen in which of the following deficiency?
67. Which of the following is true about vitamin K ? A. Vitamin K1 is synthesized in intestine by microbial
A. Thiamine B.
Pyridoxine
flora
C.
Niacin
B.
Vitamin K2 exists in plants
D. Vitamin C
C.
Vitamin K is fat soluble and require bile for its absorption
Ref: Harper 27/E p. 496-497
D. Vitamin K absorbs because it is water soluble Ref: Harper 27/E p 495
74. Exrahepatic cholestasis is associated with
A. Increased bilirubin in urine
A. Isocitrate dehydrogenase
B.
Increased urobilirubin in urine
B.
Homocysteine methyl transferase
C.
Increased bilirubin and increased urobilirubbin in
C.
Glycogen synthase
serum
D. G-6-P dehydrogenase
D. Increased bilirubin in serum
Ref: Chetterjea Shindre, 6/E, P. 181
Ref: Harper 27/E p 290-292 81. The ligand – receptor complex dissociates in the 75. Dark color urine and clay stool is seen in
endosome because;
A. Obstructive jaundice
A. Of its large size
B.
Hemolytic jaundice
B.
The vesicle looses its clathrin coat
C.
Hepatocellular jaundice
C.
Of the acidic pH of the vesicle
D. Severe dehydration
D. Of the basic pH of the vesicle Ref: Chetterjea Shindre, 6/E, P. 19
Ref: Harper 27/E p 292 82. The human plasma lipoprotein containing the highest 76. Which of the following is correct method for
percentage of triacylglycerol by weight is
estimation of true serum creatinine?
A. VLDL
A. Jaffe reaction
B.
Chylomicrons
B.
Kinetic jaffe reaction
C.
Hdl
C.
Enzyme immunoassay
D. Ldl
D. Plasma gel filtration
Ref: Lippioncott, 3/E, P. 225, 230 83. The sigma (s) subunit of prokaryotic RNA polymerase
77. For glucose estimation in blood the mode of transport
A. Binds the antibiotic rifampicin
from a primary health care center to laboratory is done
B.
Is inhibited by a=amanitin
by addition of
C.
Specifically recognizes the promoter site
A. Potassium oxalate + NaF
D. Is part of the core enzyme
B.
Potassium oxalate + citrate
C.
EDTA
84. An affected male infant born to normal parent could be an example of all of the following except;
D. Sodium citrate
A. mRNA Ref: Harper 27/E p 152 78. In all of the following RNA participates directly EXCEPT
B.
tRNA
C.
rRNA
D. snRNA Ref: Harper, 26/E, P. 356
A. Post translational modification B.
Post transcriptional modification
C.
DNA replication
85. proteins are linear polymers of amino acids, they fold into compact structures. Sometimes these folded structure associate to form homo-or hetero-dimers.
D. Splicing
Which one of the following refers to this associated from?
Ref: Harper 27/E p 38
A. Denatured state 79. All of the following procedure can be used to detect
B.
Molecular aggregation
the chromosomal mutation EXCEPT
C.
Precipitation
A. Single stranded length polymorphism
D. Quaternary structures
B.
Agarose – gel – electrophoresis
C.
Protein gradient electrophoresis
D. Pulse – field – gel – electrophoresis
Ref: Harper, 26/E, P. 34 86. The structural proteins are involed in maintaining the shape of cell or in the formation of matrices in the body. The shape of these point is
Ref: Harper 27/E p 24
A. Globular 2006 80. Vitamin B12 acts a coenzymes to which one of the following enzymes?Isocitrate dehydrogenase
B.
Fibrous
C.
Stretch of beads
D. Planar Ref: Harper, 26/E, P. 38
94. Differential expression of same gene depending on 87. Iron is present in all of the following except;
parent of origin is referred to as:
A. Myoglobin
A. Genomic imprinting
B.
Cytochrome
B.
Mosaicism
C.
Catalase
C.
Anticipation
D. Pyruvate kinase
D. Non penetrance
Ref: Harper’s, 25/E, P. 360 88. During phagocytosis, the metabolic process called
Ref: Robbins, 7/E, P. 186 95. Fluoride used in collection of blood samples for
respiratory burst involves the activation of;
glucose elimation, inhibits the enzyme
A. Oxidase
A. Glucokinase
B.
Hydrolase
B.
Hexokinase
C.
Peroxidase
C.
Enolose
D. Dehydrogenase
D. Glucose – 6 – phosphtase
Ref: Robbins, 7/E, P. 60
Ref: Harper, 26/E, P. 137 2005 2004
89. The cell component that is genetically continuous from one cell generation to the next is
96. Shine – Dalgarno sequence in bacterial mRNA is near:
A. Nuclear membrane
A. ANUG codon
B.
Golgi complex
B.
UAA codon
C.
Central Bodies
C.
AUG codon
D. Chromatin
D. UGA codon Ref: Lippincott’s Biochemistry, 2/E, P. 395
90. Golgi bodies are responsible for: A. Synthesis of plypeptides
97. A segment of Eukaryotic gene that is not represented
B.
Formation of glycoproteins only
in the mature mRNA is known as:
C.
Modification and sorting of glycoproteins
A. Intron
D. Lipid and steroid synthesis Ref: Garland’s Publishing Company, 1/E, P. 355 – 361
B.
Exon
C.
Plasmid
D. TATA Box 91. Enzymes that move a molecular group from one
Ref: Harper, 26/E, P. 253
molecule to another are known as: A. Ligases
98. An enzyme that makes a double stranded DNA copy
B.
Oxidoreductases
form a single stranded RNA template molecule is
C.
Transerases
known as:
D. Dipeptidases
A. DNA polymerase
Ref: Mathews, 3/E, P. 392
B.
RNA polymerase
C.
Reverse transcriptase
92. The amino acid residue having an imino side chain is:
D. Phosphokinase
A. Lysine B.
Histidine
C.
Tyrosine
D. Proline Ref: Harper’s, 25/E, P. 29
Ref: Harper, 25/E, P. 407 99. The transmemebrane region of protein is likely to have: A. A stretch of hydrophilic amino acids
93. Vitamin A
B.
A stretch of hydrophobic amino acids
A. Is water soluble
C.
A disulphide loop
B.
Deficiency causes impaired vision
D. Alternating hydrophilic and hydrophobic amino
C.
Maintains normal plasma of clotting factors
D. Is required for formation of clotting factors
acids Ref: Harper, 26/E, P. 450
Ref: Biochemistry by Devlin , 5/E, P. 1140, 1141 100. Which of the following group of proteins assist in the folding of other proteins:
A. Proteases B.
Proteosomes
C.
Templates
D. Chaperones
2003
Ref: Harper, 26/E, P. 507 101. Which of the following is membrane bound enzyme
107. Enzymes concerned with the citric acid cycle are found
that catalyses the formation of cyclic AMP from ATP:
in the:
A. Tyrosine kinase
A. Nucleus
B.
Polymerase
B.
Ribosomes
C.
ATP synthase
C.
Mitochondria
D. Adenylate cyclase
D. Non particulate cytoplasm
Ref: Ganong, 19/E, P. 41 Ref: Harper 25/e p. 230 102. To which of the following family of chemical mediators of inflammation, the lipoxins belong:
108. Which cell organelle contains DNA
A. Kinin system
A. Mitochondrion
B.
Cytokines
B.
Golgi body
C.
Chemokines
C.
Endoplasmic reticulum
D. Arachidonic acid metabolites
D. Centriole
103. The most abundant glycoproteins present in the
Ref: Harper 26/e p.322, 323
basement membrane is A. Laminim
109. The code world of which proteins is also an initiation
B.
Fibronectin
code for proteins:
C.
Collagen type 4
A. Leucine
D. Heparin sulphate Ref: Tencates 5/e p. 366, 368 104. A person on fat free carbohydrate rich diet continues
B.
Isoleucine
C.
Methionine
D. Lysine Ref: Lehninger 2/e p. 898
to grow obese, which of the following lipoproteins is likely to be elevated in his blood?
110. The main enzyme responsible for activation of
A. Chylomicrons
Xenobiotics is:
B.
VLDL
A. Cytochrome P – 450
C.
LDL
B.
Glutathione S- transferase
D. HDL
C.
NADPH cytochrome P – 450
Ref: Harper, 26/E, P. 211
D. Glucuronyl transferase
105. Which of the following elements is known to
Ref: Harper 25/e 780
influence the body’s ability to handle oxidative stress: 2002
A. Calcium B.
Iron
C.
Potassium
D. Selenium
111. In oxidative phosphorylation, the ATP production and respiratory chain are linked by: A. Chemical methods
Harrison 15/e 469 106. The most common condition of inherited blindness
B.
Physical methods
C.
Chemiosmotic methods
D. Confirmational changes
due to mitochondrial chromosomal anomaly is: A. Retinopathy of prematurity B.
Lebers hereditary optic neuropathy
C.
Retinitis pigmentosa
D. Retinal detachment
Ref: Lippincott 2/e p 70 112. Substrate level phosphorylation is seen in the conversion of: A. Acetoaccatate to alpha ketoglutarate
Harrison 15/e 1711, 405
B.
Succinyl CoA to succinate
C.
Fumarate to malate
D. Succinate to funarate Ref: Harper 25/e 192, 185 2001 113. The nitrogen of the body is supplied by: A. Triacyl glycerol B.
Proteins
C.
Glucose
D. Lipids
114. Collagen is rich in: A. Glutamate and glycine B.
Alanine and glycine
C.
Proline and glycine
D. Glutamate and proline
Ref: Harper 25/e 696, 697
