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GROUP MEMBERS 25 Letran. Rachelle Ann D. 26 Matias, Charmaine Joyce M. 27 Miranda, Danielle Allyson O. 28 Modina, Stella Madj Y. 29 Ortiz, Carla Jellyn 30 Panugayan, Shawn Wilgie M.
I. Title: Necessity of Oxygen in Respiration II. Materials:
germinating mung beans 25ml o f 10% KOH 25ml pyrogallic acid 50 ml distilled water 250 ml Erlenmeyer flask (2) Stopper (2) Tulle bag Carbon paper
III. Objectives: To know the necessity of the oxygen in respiration To know the the role of KOH and pyrogallic acid in the set-up. set-up. To differentiate the result between the two flasks with different solutions.
Mongo Seeds suspended in 50mL water
Procedure:
Mongo Seeds suspended in 50mL Pyrogallic acid and KOH V. Explanation:
2 tbsp of mung beans soaked overnight in water
Tulle bag with 30 pieces mung beans
Suspend 1 bag in a 50 mL KOH & pyrogallic acid in a
Suspend 1 bag in a 50 mL distlled water is a flask .
Cover both flasks with carbon paper.
The results showed that the mongo seeds suspended in the flask which contained H2Ocontinued to germinate and give rise to more roots while the mongo seeds suspended in the flask which contained pyrogallic acid (C6H6O3) and Potassium hydroxide (KOH) were not able to germinate. C 6H6O3 together with KOH absorbs the oxygen, carbon dioxide and water needed for respiration. Because the chemicals absorbed everything needed for respiration, the mongo seeds were not able to germinate. Unlike the other flask filled with distilled water, the seeds were able to germinate because nothing hinders hinders the uptake of oxygen. oxygen. VI. Conclusion
Put the flasks in a dark chamber for two days
Record the results.
Plants need oxygen in respiration and the mung beans in the flask containing pyrogallic acid and KOH absorbed absorbed the oxygen oxygen needed by the plants, therefore the mongo seeds were not able to germinate. The flask that was filled with water did not contain any chemical that hinders the uptake of oxygen so the mongo seeds germinated. Therefore, the mongo seeds need oxygen in order to germinate. These types of plants that need oxygen in order to germinate are called strict or obligate aerobes.
IV. Results:
GERMINATION GERMINATION
H2o
Pyrogallic acid and KOH
Germinated Germinated
Did not germinate
VII. References http://www.henriettesherbal.com/eclectic/bpc19 11/acidum-pyro.html http://chemindustry.ru/Potassium_Hydroxide.ph p Botany: An Introduction to Plant Biology by James D. Mauseth
