Microbiology Practice Test I

MCQ-PG Series

Practice Set I

PG/MICRO/I Time: 60 min

Microbiology

Email:[email protected] Total Marks: 100 | Pass Mark: 60

ClinicalMicrobiology MCQTestSeriesbasedonPostGraduateExamPreparation.Readthe MCQTestSeriesbasedonPostGraduateEx amPreparation.Readtheinstructions instructions atthebeginingcarefully.ThisisaSample atthebeginingcarefu lly.ThisisaSamplePracticePaperonClin PracticePaperonClinicalMicrobiology icalMicrobiology.. Please Note: There is no Negative Marking for incorrect responses.

Multiple Choice Questions

1. Human Human cytomegalo cytomegalovirus virus (CMV) is a member member of the family: family: A Adenoviridae

C Herpesviridae

B Coronaviridae

D Picornaviridae

2. All of of the the followin following g are RNA viruses viruses except: except: A Rhabdoviruses

C Parvoviruses

B Orthomyxoviruses

D Picornaviruses

3. Fish virus virus or, viru viruses ses of fish include: include: A Coltivirus

C Lymphocryptovirus

B Lymphocystivirus

D Corticoviridae

4. The virus virus that that causes Chickenpox Chickenpox belong belongss to the family: family: A Orthomyxoviridae

C Poxviridae

B Paramyxoviridae

D Herpesviridae

5. Which of the the following following antiviral agents is is a deoxycytidin deoxycytidinee analog? A Foscarnet

C Lamivudine

B Ganciclovir

D Cidofovir

6. Indirect Indirect immun immunofl ofluo uorescence rescence is used to iden identify tify the the presence of A Treponema pallidum antibodies

C enteropathogenic Escherichia coli

meningitid itidis is B Neisseria mening

D Listeria monocytogenes

7. Intranu Intranuclear clear inclusion inclusionss are characteristics of all of the the following following viruses viruses except: A Cytomegalovirus

C Mumps virus

B Measles virus

D Varicella-zoster virus

Basic Medical Sciences

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2017

MCQ-PG Series

Practice Set I

Microbiology

8. Humans Humans are the only only natural natural hosts hosts of all of the following following viruses viruses except: e xcept: A Measles virus

C Variola virus

B Flavivirus

D Mumps virus

9. Peplom Peplomers ers are found found on viral: viral: A Capsomere

C Envelope

B Capsid

Nucleocapsid D Nucleocapsid

10. Which of the the following following is not not a characteristic charac teristic property of of Polyom Polyomaviruses aviruses (BK virus)? belongss to Parvovir Parvoviridae idae family A belong

C late region codes for SV40 large tumor (T) antigen

B circular double-stranded DNA

D nonenveloped, icosahedral, 45 nm in diameter

11. Precipi Precipitati tation on reaction: reaction: precipitin band band formed formed at the zone of A precipitin equivalence

C monoclonal antibodies are used in precipitin precipitin reactions reactions

precipitin band band formed formed at the zone of B precipitin antigen excess

D zone of antibody excess exhibits optimal ratio for precipitation

12. A virus virus etiologically etiologically linked linked to Burkitt’s Burkitt’s lymph lymphom oma: a: A Rous sarcoma virus

C Human herpesvirus 8

B Epstein-Barr (EB) virus

D Cytomegalovirus

13. Molluscum Molluscum contagiosum contagiosum belongs belongs to which virus group group?? A Adenovirus

C Coronavirus

B Herpesvirus

D Poxvirus

14. Acute poststrept poststreptoco ococcal ccal glomeru glomerulon loneph ephritis: ritis: A characterized by low serum complement levels

C lumpy deposit of complement component C5

B may follow group A ß-hemolytic streptococcal infection

D immune complex-mediated type IV hyperse hypersensitivity nsitivity reac tion

15. The major major immun immunoglo oglobu bulin lin of of B cell receptor re ceptor (BCR) for appropriate appropriate antigens is: A IgE

C IgD

B IgG

D IgM

16. The test to determine Minimal Minimal inhibitory inhibitory concentration concentration (MIC) employ employss what method/techniqu method/technique? e? A Dilution technique

C Western blot technique

B Disk-agar diffusion method

D Subculturing technique


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2017

MCQ-PG Series

Practice Set I

Microbiology

17. Which of the the following following are good good disinfectant agents? A Chlorine

C Alcohols

B Hydrogen peroxide

D Aldehydes

18. Regarding Regarding Polio Poliovaccine vaccine correct is/are: A Killed-virus vaccine produces secretory IgA antibodies

C Live oral poliovaccine induces humoral antibodies

B Oral polio vaccine contains Killed-virus

D Oral polio vaccine contains live attenuated virus

19. The virus virus that causes Molluscum Molluscum Contagiosum Contagiosum belongs belongs to which family? family? A Papovaviridae

C Parvoviridae

B Paramyxoviridae

D Poxviridae

20. An agent agent that that is a IL-1 receptor receptor antagon antagonist: ist: A Daclizumab

C Anakinra

B Etanercept

D Sirolimus

21. Which of the the following following viruses viruses has Single-stranded DNA in its genom genome? e? A Reoviridae

C Poxviridae

B Parvoviridae

D Adenoviridae

22. Which of of the following following immu immuno nosupp suppress ressant ant drugs drugs is a Calcineurin Calc ineurin inhib inhibitor? itor? A Infliximab

C Anakinra

B Tacrolimus

D Sirolimus

23. Correct Correct abou aboutt T-help T-helper er TH1 cells: produce ce IL-4 A produ

produce ce IL-2 C produ

produce ce cytokin cytokines es involv involved ed in humoral humoral B produ immunity

produce ce cytokin cytokines es involv involved ed in cellul c ellular ar D produ immunity

24. Rabies viru viruss belong belongss to which genera? genera? A Rubivirus

C Lyssavirus

B Morbillivirus

D Orbivirus

25. Which of of the following following is immu immunosu nosupp ppress ressive ive agents is an anti-CD3 antibody antibody?? A Anakinra

C Etanercept

B Tacrolimus

D Muromonab


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2017

MCQ-PG Series

Practice Set I

Microbiology

26. Drug of of choice for chloroq chloroquin uine-resistant e-resistant falciparum falciparum malaria: malaria: A Artesunate

C Quinidine gluconate

B Mefloquine

D Primaquine

27. A virus virus responsible responsible for causing nephrop nephropathy athy in renal transplant recipients: A Phlebovirus

C Polyomavirus

B Papillomavirus

D Papovavirus

28. All of the the following following agents are useful to treat the donor donor tissue before transplant in order order to reduce Graft-versus-host (GVH) reaction except: A Monoclonal antibodies

C Interleukin (IL)-2

B Antithymocyte globulin

D Cyclosporine

29. The most most useful techniqu techniquee for examining examining outbreaks outbreaks in a hospital hospital A Plasmid analysis

C Ribotyping

B Multilocus enzyme electrophoresis

ac id sequence sequence analysis analysis D Nucleic acid

30. Which Which of the the following following is is not a granulocy granulocyte? te? Neutrophils ils A Neutroph

C Monocytes

B Eosinophils

D Basophils

31. Which of the the following following is immu immunosu nosupp ppress ressive ive agents is a IL-2 receptor antagonist? antagonist? A Adalimumab

C Muromonab

B Infliximab

D Daclizumab

32. When When a mosqu mosquito ito takes takes a blood blood meal, it either either A injects gametocytes or ingests sporozoites

C ingests gametocytes or injects sporozoites

B ingests gametocytes or injects trophozoites

D injects gametocytes or ingests trophozoites

33. The mechanism of disk diffusion diffusion method method is employed employed to: to: A estimate the bactericidal effects of an antibiotic

C measure the ability of drugs to inhibit bacterial growth growth

B measure the concentration necessary to inhibit bacterial growth

D determine the susceptibility of an isolate


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2017

MCQ-PG Series

Practice Set I

Microbiology

34. Regarding Regarding antibod antibodies, ies, spot the the incorrect statements: statements: A antibodies make up about 35% of plasma plasma proteins proteins

C antibodies are formed by B-lymphocytes and plasma cells

B antibodies do not penetrate into cells

D immature B cells carry IgM on their surface

35. Regarding Regarding Pulsed-fi Pulsed-field eld gel gel electrophoresis, electrophoresis, correct statements statements is/are: A allows the separation of DNA fragments upto 10 kbp

C allows hybridization of DNA to RNA D allows the separation of DNA fragments upto 100 kbp

B allows nucleic acid amplification

36. What What differentiates differentiates Sanitization Sanitization from from Sterilizatio Sterilization? n? A Sanitization reduces the number of pathog pathogenic enic microbes microbes

C Sterilization keeps microbes under control by reducing their numbers

B Sterilization involves procedures inhibiting microbial growth

D Sanitization means absence of living microbes, including spores and viruses

37. Regarding indirect indirect solid-phase solid-phase enzyme immu immunoso nosorbent rbent assay ass ay (SPIA): (SPIA): A results in a fluorescent end product

C enzyme substrate binds with the second antibody

B enzyme is conjugated with the third antibody

D enzyme is conjugated with the first antibody

38. Interleukin (IL) required required for for stem cell differentiation differentiation into into T and B cells: A IL-5

C IL-7

B IL-2

D IL-6

39. The The second signal signal required required for TH cell stimulation/proliferation involves: A CD3 complex associated with T cell receptor

C antigen-MHC complexes D CD28 protein receptor on T H1 cell

protein called B5 (CD87) (CD87) B protein

40. A drug drug that that causes cause s radical cure by eliminating eliminating exoerythro exoerythrocytic cytic forms forms of malaria malariall parasites in the liver: A Halofantrine

C Chloroquine phosphate

B Quinine sulfate

D Primaquine

41. A nucl nucleot eotid idee analo analog: g: A Acyclovir

C Cidofovir

B Ribavirin

D Lamivudine


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2017

MCQ-PG Series

Practice Set I

Microbiology

42. The The number number of capsomeres capsomeres in the the capsid ca psidss of Epstein-Barr Epstein-Barr virus virus (EBV) 252 A 252

162 C 162

B 72

180 D 180

43. Which of the the following following organ organ transplants have have the highest highest rate of success? success ? A Bone marrow

C Heart

B Cornea

D Kidney

44. What is not not correct abou a boutt minimu minimum m inhib inhibitory itory concentration concentration (MIC)? (MIC)? A Tube dilution assay is employed

C lowest concentration that results in bacterial death

B it is determined based on turbidity

D lowest concentration that prevents growth of a bacterium

45. Rifampin binds binds strongly strongly to which of of the following following bacte bacterial rial enzymes? A DNA-dependent DNA polymerase

C RNA-dependent RNA polymerase

B RNA-dependent DNA polymerase

D DNA-dependent RNA polymerase

46. Which of of the following following drug drugss is an a n example example of Quinoline-meth Quinoline-methanol? anol? A Tafenoquine

C Chloroquine

B Quinine

D Mefloquine

47. The causative organism of Poliom Poliomyelitis, yelitis, Polioviruses Polioviruses belongs belongs to the the family A Togaviridae

C Caliciviridae

B Adenoviridae

D Picornaviridae

48. Which Which of the the following following is a reverse transcriptase transcriptase inhibit inhibitor? or? A Ritonavir

C Cidofovir

B Didanosine

D Vidarabine

49. What What is not correct correct regarding regarding bacterial endosp endospores? ores? A resistant to autoclave

C resistant to desiccation

B not visible with light microscope

D resistant to heat, UV rays and gamma radiation

50. Epstein-B Epstein-Barr arr viru viruss belong belongss to: to: A Papillomaviridae

C Herpesviridae

B Picornaviridae

D Flaviviridae


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2017

MCQ-PG Series

Practice Set I

Microbiology

51. Which of the following pathogens have no vectors? A B henselae

moniliformis C S moniliformis

B B pertussis

D B bacilliformis

52. Measles virus virus of hum humans ans belong belongss to A Rubulavirus genus

C Henipavirus genus

B Morbillivirus genus

D Orthobunyavirus genus

53. Drug useful useful for the the treatment of of Hepatitis C virus virus infection infection:: A Famciclovir

C Tenofovir

B Didanosine

D Ribavirin

54. The environm environment ent at the the bottom bottom of a deep deep river or lake: A Photic zone

C Benthic zone

B Abyssal zone

D Littoral zone

55. UV energy energy most most destructiv destructivee to bacterial cells: A 360 nm

C 460 nm

B 265 nm

D 232 nm

56. Correct about about Minimal Minimal inhib inhibitory itory concentration (MIC): A quantitative testing that uses a microdilution technique

C qualitative testing that uses a microdilution technique

B quantitative testing that uses disk-diffusion method

D qualitative testing to monitor resistance to an antibiotic

57. Which of of the following following techniques techniques is best bes t suited for genotyp genotyping ing and genetic fingerprinting fingerprinting?? Northern blot blot densitometr densitometry y A Northern

C Electroporation

B Southern blot analysis

D Pulsed-field gel electrophoresis

58. Anti-malaria Anti-malariall drug(s) drug(s) that does does not not prevent prevent relapse and has has no gametocidal gametocidal activity: A Artemisinin

C Chloroquine

B Mefloquine

D Primaquine

59. Type Type of glass glass con c ontainer tainer suitable for non-aqu non-aqueous eous preparations preparations for parental use: A Type II glass containers

C Type I glass containers

B Type IV glass containers

D Type III glass containers


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2017

MCQ-PG Series

Practice Set I

Microbiology

60. All of the following following viruses viruses have Single-stra Single-strand nded ed RNA as their genom genomee material except: A Togaviridae

C Picornaviridae

B Herpesviridae

D Flaviviridae

61. A biolog biological ical immuno immunosupp suppress ressant ant agent which is a TNFa inhibitor: inhibitor: A Anakinra

C Muromonab

B Tacrolimus

D Etanercept

62. Linear, single stranded, positive strand RNA viruses include: A Epstein-Barr virus

C Togaviruses

B Polyomaviruses

D Reoviruses

63. 63. T-h T-help elper TH2 cells produces all of the following cytokines except: A IL-2

C IL-4

B TNF-ß

D IL-5

64. Which of the the following following obligate obligate intracellular parasites a re not cells and a nd do not not have a nucleus? nucleus? A Viruses

C Bacteria

B Protozoa

D Fungi

65. The causative agent of Venezuelan hemorrhagic fever is: A Machupo virus

C Guanarito virus

B Sabia virus

D Junin virus

66. The virus virus that that causes Korean Korea n hemorrh hemorrhagic agic fever belongs belongs to which genus? genus? A Alphavirus

C Hantavirus

B Orbivirus

D Flavivirus

67. Lactoferri Lactoferrin n is is found found in A Basophil

C Monocyte

Neutrophil il B Neutroph

D Eosinophil

68. Incorrect about infectious infectious mono mononucleosis nucleosis caused by hum human an cytomegaloviru cytomegaloviruss (HCMV): A site of latency is Trigeminal sensory ganglia

heterophilee antibod antibodies ies C presence of heterophil D HCMV is the most common intrauterine infection

B site of latency is monocytes and lymphocytes


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2017

MCQ-PG Series

Practice Set I

Microbiology

69. Regarding Regarding retroviru retroviruses, ses, spot spot the incorrect incorrect statements: statements: A linear positive sense, single-stranded RNA

C contains reverse transcriptase enzyme D spherical enveloped viruses

B circular negative sense, single-stranded RNA 70. Mump Mumpss virus virus belon belongs gs to: to: A Paramyxovirviridae

C Parvoviridae

B Herpesvirviridae

D Orthomyxoviridae

71. Which Which of the followin following g sterilization sterilization processes processes readily inactivates inactivates (kills) (kills) Spores? Spores? A Autoclave

C Boiling

B Ultraviolet light

D Desiccation

72. Compo Componen nentt of plasma plasma sterilization sterilization system: system: A Gamma ray irradiation

C UV irradiation

B Autoclave

D Vapor-phase systems

73. Which of the the following following anti-malarial drugs drugs is classified classifie d as 4-Amino 4-Aminoqu quino inolines? lines? A Chloroquine

C Primaquine

B Mefloquine

D Pyrimethamine

74. In the the USA, S aureus is resistant res istant if the minim minimum um inhibito inhibitory ry concentration concentration (MIC) is: A MIC is = 2 µg/mL

C MIC is =16 µg/mL

B MIC is = 4 µg/mL

D MIC is =8 µg/mL

75. Among Among the following following agents agents responsible responsible for congenital congenital defects in fetus, which one one is the lytic virus that may cause fetal death? A Cytomegalovirus

C Varicella-zoster

B Herpes simplex

D Rubella virus

76. 'Verruga peruana' is most commonly associated with A Oroya fever

C Pontiac fever

B Venezuelan hemorrhagic fever

D Haverhill fever

77. Regarding Pulsed-field gel electrophoresis, electrophoresis, which of the following following statements state ments is/are correct? correc t? A uses a thermal cycler with an illuminator to detect fluorescence

C uses a constant electrical field with an uniform voltage gradient

B used for separation of large DNA molecules

D used for separation of small RNA molecules


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2017

MCQ-PG Series

Practice Set I

Microbiology

78. Which Which of the the following following species species is alpha-hemo alpha-hemolyt lytic? ic? intermedius A Streptococcus intermedius

C Listeria monocytogenes

B Arcanobacterium pyogenes

D Erysipelothrix rhusiopathiae

79. Which Which of of the follo following wing is a DNA virus? virus? A Coronaviruses

C Polyomaviruses

B Picornaviruses

D Togaviruses

80. Drug useful useful in the the treatment of Human Human cytomegaloviru cytomegaloviruss (HCMV) infection: A Ribavirin

C Lamivudine

B Ganciclovir

D Oseltamivir

81. Respiratory Respiratory syncy syncytial tial virus virus (RSV) (RSV) is a member member of A Togaviridae

C Orthomyxoviridae

B Paramyxoviridae

D Herpesviridae

82. Which of the the following following statements regarding Trivalent oral oral poliov poliovacc accine ine are correct? A Trivalent oral poliovaccine is the Salk vaccine

C Immune globulin is highly effective following infection

B Should not be administered to an immunosuppressed individual

D Pregnancy is not a contraindication

83. What is the the generality and common common among among interleukin interleukin (IL-1) and interleukin (IL-8)? A Both IL-1 & IL-8 are derived from monocytes

C Both IL-1 & IL-8 are chemoattractant for eosinophils

B Both IL-1 & IL-8 are endogenous pyrog pyrogens ens

der ived from D Both IL-1 & IL-8 are derived macrophages

84. MethylMethyl-accepting accepting chemo chemotaxis taxis proteins proteins (MCPs) (MCPs) include include all except: A sucrose

C serine

B galactose

D maltose

85. Which Which of of the follo following wing organism organismss is an Yeast? A Listeria monocytogenes

C Chlamydia trachomatis

B Candida albicans

D Toxoplasma gondii

86. Which of the the following following drug drugss inhibits inhibits the virus virus DNA polymeras polymerase? e? A Zidovudine

C Didanosine

B Saquinvir

D Acyclovir


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2017

MCQ-PG Series

Practice Set I

Microbiology

87. Rubella Rubella viru viruss belong belongss to: to: A Orthomyxovirus group

C Togavirus group

B Paramyxovirus group

D Coronavirus group

88. The causative organism organism of of Progress Progressive ive multifo multifocal cal leukoencephalopathy leukoencephalopathy (PML): A Papovavirus

C Polyomavirus

B Papillomavirus

D Parvovirus

89. All of the following following are Spore-forming Spore-forming microorganism microorganismss except: A Erysipelothrix rhusiopathiae

C Myxococcus fulvus

Bacillus cereus B Bacillus

D Thermoactinomyces vulgaris

90. A gram-positive gram-positive bacte bacterium rium whose normal normal habitat habitat is cow dung dung:: A Caryophanon

C Brevibacterium

B Myxobacteriales

D Thermoactinomyces

91. Which Which of of the follo following wing is a T-cell growth growth factor? factor? A IL-1

C IL-4

B IL-2

D IL-6

92. Which of the the following following viruses viruses con c ontains tains an RNA-dependent RNA-dependent DNA polym polymeras erase? e? A Smallpox virus (variola)

C Rous sarcoma virus

B Hepatitis B virus

D Poliovirus

93. Rift Valley fever fever is due to the the virus virus that belong belongss to genus genus A Phlebovirus

C Flavivirus

B Hantavirus

D Orthobunyavirus

94. Which of of the following following is the the most effective tool for condu conducting cting Genotyp Genotyping ing assays? A RT-qPCR

C Multiple-locus VNTR analysis

Nucleotidee sequence sequence analysis analysis B Nucleotid

D Restriction fragment length polym polymorp orphism hism

95. Linear, double-stranded DNA virus includes all of the following except: A Herpesviruses

C Adenoviruses

B Picornaviruses

D Cytomegalovirus


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2017

MCQ-PG Series

Practice Set I

Microbiology

96. All of the following following viruses viruses are a re associated ass ociated with hemagglu hemagglutinating tinating activity except: A Pneumovirus

C Rubulavirus

B Respirovirus

D Adenovirus

97. A method method suitable for both both qualitative qualitative and quantitative quantitative analysis A Crossed immunoelectrophoresis

C Electroimmunoassay

B Immunoelectrophoresis

D Counter-immunoelectrophoresis

98. 'Woolsorter’s disease' is caused by: A Rhodococcus equi

Clostridium botulinum C Clostridium

Erysipelothrix rhusiopathiae B Erysipelothrix

Bacillus anthracis D Bacillus

99. Which of the the following following techniques techniques was the first in nucleic acid isolation, amplification, amplification, and sequencing? A restriction endonuclease analysis

plasmid profile profile analy a nalysis sis C plasmid

B ribotyping

D nucleic acid sequence analysis

100. A form of cell wall growth growth characterized characterized by cell wall synthesis synthesis at the septum septum and at the equatorial rings is exhibited by: A E coli

C S pneumoniae

B B subtilis

D S aureus


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2017

MCQ-PG Series

Practice Set I

Microbiology

Disclaimer: Every attempt has been made to validate each and every question/answer. All remaining errors are deeply regretted. Author will not be held responsible for any errors. Please consult the standard textbooks given in the reference section for any discrepancy. Questions with wrong answers may better be left unanswered. This is a practice series available free to download, and designed for the benefit of prospective PG candidates and UG medical students alike.

Reference Textbooks: 1. Medical Microbiology: Jawetz, Melnick & Adelberg's, 24th Edition, Lange McGraw-Hill publi publication cationss 2. Microbiolo Microbiology: gy: Presc Prescott ott M. Lansing Lansing (Ed): (Ed): Prescott- Harley- Klein, 5th Edition Edition McGraw-Hill McGraw-H ill publi publication cationss


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2017

Microbiology Practice Test I

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