Vegasys Service Manual Draft

VEGASYS

Service Manual

PRELIMINARY

Via E. Barsanti 17/A 00012 Guidonia – ROME (ITALY) www.ams-analyzers.com

CONFIGURATION CONFIGURATIO N SHEET

CAPITOLO

DESCRIZIONE

REV.

INDEX

TABLE OF CONTENTS

00

01

INTRODUCTION

00

02

GENERAL DESCRIPTION

00

03

INSTALLATION

00

04

ELECTRICAL SCHEMES AND DRAWING

00

05

DIAGNOSTIC PROGRAM

00

06

MAINTANANCE

00

07

SETTINGS AND ADJUSTMENTS

00

08

ERROR SIGNALING

00

09

HOST

00

10

TROUBLESHOOTING

00

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INDEX

TABLE OF CONTENTS

Chapter 01

INTRODUCTION

Chapter 02

GENERAL DESCRIPTION

Chapter 03

INSTALLATION INSTALLAT ION

Chapter 04

ELECTRICAL SCHEMES AND DRAWING

Chapter 05

DIAGNOSTIC PROGRAM

Chapter 06

MAINTANANCE

Chapter 07


Chapter 08

ERROR SIGNALING

Chapter 09

HOST

Chapter 10

TROUBLESHOOTING TROUBLESHOOTIN G

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Chapter 01 – INTRODUCTION

CHAPTER 01 - INTRODUCTION -

INDEX

1

INTRODUCTION .................................................................................................................... 2

1.1

THE AIM OF THE TECHNICAL MANUAL ......................................................................... 2

1.1.2 MANUAL OUTLINE............................................................................................................... 2 1.2

SYSTEM INTRODUCTION.................................................................................................... 4

1.3

PRECAUTIONARY MEASURES .......................................................................................... 5

1.3.1 CHEMICAL RISKS ................................................................................................................. 5 1.3.2 ELECTRICAL RISKS.............................................................................................................. 5 1.3.3 MECHANICAL RISKS............................................................................................................ 5 1.4

GUARANTEE .......................................................................................................................... 6

1.5

TECHNICAL OPERATING FEATURES .............................................................................. 7

1.5.1 COMPUTER & SOFTWARE FEATURE ............................................................................... 8 1.5.2 DIMENSIONS, WEIGHT & ENVIRONMENT......................................................................9 1.5.3 INSTALLATION REQUIREMENTS...................................................................................... 9

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1

INTRODUCTION

1.1

THE AIM OF THE TECHNICAL MANUAL

This manual has been written in order to supply the technical staff, the persons who are responsible for the maintenance and for resolving instrument failures, a complete and detailed guide of the Vegasys analyzer, in accordanance with the standard UNI EN591 (which requires a manual to be supplied with vitro diagnostic instruments for professional use).

1.1.2

MANUAL OUTLINE

The technical manual is composed of 10 chapters that not only describe the operative technical characteristics of the system, but also the reparation and maintenance procedures to use for the modules that they are composed of. .

Chapter 1

INTRODUCTION

The manual structure is described, and recommendations are given regarding the general use of the analyzer. The technical characteristics of the operative system are given in this chapter.

.

Chapter 2

DESCRIPTION OF THE SYSTEM

Describes the system, in particular, the analytical cycle of the single modules and electronic boards.

.

Chapter 3

INSTALLATION

Describes the unpacking procedures and the required characteristics for the place of installation.

.

Chapter 4

ELECTRONICS SCHEMES AND DESIGNS

Gives the electronics schemes and assembly designs of the electronics board and the assembly designs of the modules that the system is composed of.

.

Chapter 5

DIAGNOSTIC PROGRAM

Describes the folders in the Diagnostic Program that are responsible for the checks and the calibration of the modules in the system.

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.

Chapter 6


Gives the procedures for substitution, adjustment and check of the system modules.

.

Chapter 7

MANTENANCE

Describes the routine maintenance that needs to be done on a regular basis in order to guarantee the correct functioning of the analyzer.

.

Chapter 8

COMUNICATION WITH HOST

Describes the communication parameters and the ASTM protocol specifics used by the Vegasys system when connecting to the Host Computer.

.

Chapter 9

ERROR SIGNALS GUIDE TO RESOLVE ANOMOLIES

Lists the system’s error signals, describes the errors in the results.

.

Chapter 10

ANOMALIES GUIDE

Gives a guide for how to resolve anomalies.

The technical staff responsible for resolving the structural anomalies is highly suggested to thoroughly examine the contents of this manual before operating the system.

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1.2

SYSTEM INTRODUCTION

The “Liasys” system is a continuous-load, random access, bench top instrument for performing chemical and immuno-turbo-dymetric clinical analysis. It is totally automatic

and computer

controlled.

INSERIRE IMMAGINE DEL VEGASYS

Via E. Barsanti 13/A 00012 Guidonia – ROME (ITALY)

Via E. Barsanti 17/A 00012 Guidonia – ROME (ITALY)

Sales & Technical Assistance

Vegasys

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Manufacturer

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1.3

PRECAUTIONARY MEASURES

1.3.1

CHEMICAL RISKS

The individual operator is responsible for assuring that all possible precautionary measures are taken against eventual risks associated with the use of the “Vegasys” instrument in clinical laboratory settings. The manufacturer will provide the reagents kit and specific written information on the use of each of the reagents. It is important that the samples be well coagulated and then carefully centrifuged. Samples which contain fibrinogen clots can obstruct the probe and lead to inexact sampling. If blood samples containing gel are used, it is suggested that the manufacturer’s recommendations be followed. Immediately clean and remove any accidental leakage of reagent or other liquid.

1.3.2

ELECTRICAL RISKS

As with any electrical device, the risk of electric shock exist. Is therefore necessary to take every precautionary measure possible when working with this, or any other, electrical instrument to avoid contact with power supply wires, electrical components or electronic boards. 1.3.3

MECHANICAL RISKS

Several precautionary measures should be taken when operating the analyzer: avoid wearing very loose clothing or jewelry that could become tangled in the instrument’s moving

parts (e.g. the sampling probe); whenever possible, operate the instrument with the main cover panel lowered.

WARNING: Never attempt to service or substitute any part(s) of the analyzer when the instrument is turned on.

Any and all technical repairs or servicing must be performed by specialized personnel only.

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1.4

GUARANTEE

AMS guarantees the substitution of all defective components and/or materials for a period of time not above of 14 months starting from the date of invoicing. Saying guarantee, as well as Technical Assistance, generally is intended furnished as net ex factory Rome. This guarantee does not include consumable and instrument parts in contact with liquids. All components not included in the guarantee are reported in the following table. Besides guarantee does not cover damage caused by : -

improper use of the VEGASYS instrument (or however not according to the Producer or Seller instructions)

-

bad transport

-

insufficient (or missing) preventive maintenance by the User

In particular any damages due to the transport must be immediately reported to the carrier when he delivers. CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF GUARANTEE Description

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Service Manual

Type

Quantity

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1.5

TECHNICAL OPERATING FEATURES ♦

Open and Fully Automatic, Counter Top, Batch Analyzer for VET and Clinical Laboratoty. It runs immunoturbidimetric, clinical chemistry analyses using single or double reagent methodologies.

ASSAY TYPE

♦

End Point, Initial Rate, Kinetic, Bichromatic, Differential,

TEST ENTRY MODE

♦

Batch, Profiles.

THROUGHPUT

♦

Up to 200 colorimetric test/hr.

WORKING TEMPERATURE

♦

37° C

♦

1 removable reagent try for 18 reagent containers (the reagent try can be easily removed for refrigeration).

REACTION RCKS

♦

Six racks of 19 reaction cells for a total of 114 cells.

STANDARD & CONTROLS LOADING

♦

One rack for 6 standard and 2 controls.

♦

Four racks for a total of 40 samples.

♦

Minimum

450 µl

♦

Maximum

XXX µl

♦

The sampling arm contains the sampling probe that aspirates the reagent and the sample to be analysed and transfer them into the reaction cell for incubation.

♦

The aspiration arm transfer the reaction mixture from the reaction cell of the photometric system for measurement.

♦

Integrated module without syringe with the following specifications:

DESCRIPTION

ON LINE REAGENTS

SAMPLE LOADING REACTION VOLUME

SAMPLING & ASPIRATION ARM

DILUTER

OPTIC SYSTEM & MICRO FLOW CELL

Vegasys

Service Manual

♦

•

Diluter volume: 1000 µl

•

Sample volume: 3.0 µl ÷ 99 µl (1 µl incr.)

•

Precision: CV < 1%

The photometer system consists of a narrow bandwidth interference filters of 340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 576 nm, 620 nm.

♦

Light Source: halogen lamp 6V/10 W

♦

Linear range: 0.001 ÷ 2.5 Abs

♦

Resolution: 0,001 Abs

♦

Micro Flow Cell Volume: 50 µl

♦

Temperature: 37° C – Peltier effect

♦

Carry Over: 2% nominal for total volume of 450 ÷ 500 µl of solution.

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OPTICAL PATH

♦

10 mm.

♦

Six racks of 19 reaction cells for a total of 114 cells.

♦

Incubation temperature: 37°C

REACTION PLATE

1.5.1 COMPUTER & SOFTWARE FEATURE TYPE

♦

IBM Compatible

CPU

♦

Pentium III 300 MHz, 512 Kb Cache or plus

♦

RAM 256 Mb or plus

♦

Hard Disk 20 Gb or plus

♦

RAM Video: 4 Mb

♦

Colour SVGA 15’’ low radiation

MEMORY

MONITOR

Resolution 1024 x 768 pixels; max number of colors 65536 (16 bit) ♦

Windows compatible.

♦

Standard (101 Keys) – PS2

♦

Host : RS 232 Serial Port

♦

Instrument Link : RS 232 Serial Port

♦

SOFTWARE AVAILABLE LANGUAGES

Multitasking WINDOWS XP

♦

Italian, English.

SETTINGS

♦

Disable all the energy saving options Disable the screen saver

PRINTER & KEYBOARD

INTERFACE

♦

NOTE: Even though the computers demonstrate the same technical and operative characteristics,

some of these could have different hardware installed. This could cause problems for the Vegasys software when running tests (A message appears indicating “Random” error or blocks the program). Therefore, if the PC is bought separately/locally, it is highly recommended to test the system at your offices before preceding with the installation at the final client’s. Consequently, AMS denies any responsibility for software problems that are due to buying the computer separately from the instrument.

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1.5.2

DIMENSIONS, WEIGHT & ENVIRONMENT ♦

Height:

32 cm

♦

Depth:

45 cm

♦

Length:

WEIGHT

♦

65 Kg

OPERATING ENVIRONMENT

♦

Temperature: 18°C ÷ 32°C.

♦

Relative humidity: 20% ÷ 85%

DIMENSION

1.5.3 POWER REQUIREMENTS

50 cm

INSTALLATION REQUIREMENTS ♦

Input Voltage 110

♦

Input Frequency: 47 ÷ 63 Hz

♦

Power consumption: ♦ ♦

÷

240 Vac

180 W for the analytical unit 600 W for the work station

SAFETY REGULATIONS

ELECTROMAGNETIC COMPATIBILITY

♦

Warning: A steady power supply (+ 10%) must be provided for the instrument.

If it is not, the manufacturer highly recommends the use of:

Vegasys

♦

UPS Uninterruptible Power Supply (1 KW)

♦

ELECTRONIC STABILISER

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Chapter 02 - General Description

CHAPTER 02 – GENERAL DESCRIPTION

INDEX

2.1

SYSTEM DESCRIPTION..................................................................................................2

2.2

MAIN COMPONENTS OF THE ANALYSER.................................................................3

2.2.1 ANALYTICAL PLATE .....................................................................................................3 2.2.2 SAMPLING SYSTEM .......................................................................................................4 2.3

SAMPLING ARM ..............................................................................................................4

2.4

ASPIRATION ARM ...........................................................................................................4

2.5

PROBE CLEANER ............................................................................................................5

2.6

WASHING WELL .............................................................................................................5

2.7

INCUBATOR .....................................................................................................................5

2.8

PERISTALTIC PUMP .......................................................................................................6

2.9

PHOTOMETRIC SYSTEM ...............................................................................................7

2.10 MICROFLOW CELL .........................................................................................................8 2.11 COMPUTER & SOFTWARE FEATURE .........................................................................9 2.11.1 TECHNICAL & OPERATIVE FEATURES...................................................................10 2.12

DIMENSION, WEIGHT & ENVIRONMENT ...............................................................11

2.13

INSTALLATION REQUIREMENTS ............................................................................11

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2.1

SYSTEM DESCRIPTION

The Vegasys is an Open, Fully Automatic Counter Top, Batch Analyzer for VET and Clinical Laboratory. It runs immunoturbidimetric, clinical Chemistry analysis using sngle or double reagent methodologies. The first time the system is used for laboratory analyses, the user must configure the system based on the specific needs of that laboratory; i.e.: the chemistry parameters and the reagents rack, along with the normal, calibrated and control values must be defined. NOTA: In order to assure the analytical performances of the system “Instrument + reagents”,

it is responsability of the laboratory staff to use reagents, controls, calibrators validated on the “Vegasys”, or in alternative, to qualify other reagents, controls and calibrators validated on the “Vegasys”, or in alternative, to qualify other reagents, controls and calibrators in compliance with the applicable relations.

The daily routine analyses will be carried out according to patient sample arrival in a sequential manner. The work list is organized using loading rack holding up to 10 patient samples. In order to access the main program, double click on the “Vegasys” icon on the computer desktop. The main menu – “System Monitor” – will appear.

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2.2

MAIN COMPONENTS OF THE ANALYSER

2.2.1

ANALYTICAL PLATE

The analytical plate is the main movable part of the analyser. The instrument is equipped with a 18 reagents plate (see Figure 1). It is provided with: six racks of 19 reaction cells for a total of 114 cells; 



one rack for 6 standards and 2 controls;



four racks for a total of 40 samples;



one removable reagent tray for 18 reagent containers (the reagent tray can be easily removed for refrigeration).

Figure 1: Analytical Plate

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The maximum volume capacity of the containers for the 18 reagents analytical plate are as follows:



Volume of reaction cell: 1.2 ml



Volume of sample cup: 0.8 ml



Volume of reagent containers : 35 35 ml ml

The basic operating cycle takes 18 seconds. The reactions take place at 37°C. This temperature is maintained constant by a controlled heading unit placed around the reaction plate.

2.2.2

SAMPLING SYSTEM

The sampling system consists of a highly precise diluter controlled by a microprocessor and has the following specifications: Diluter volume

1000 µl

Sample volume

from 3 to 99 µl with 1 µl increment

Accuracy

>99%

Materials in contact with the solutions

Teflon & glass

Precision

CV < 1 % a 3 µl

The recommended minimum sampling volume for analysis is 5 µl and for reagent volume is 400 µl. There is also an automatic wash cycle in between tests to avoid the risk of crosscontamination.

2.3

SAMPLING ARM

The sampling arm contains the sampling probe that aspirates the reagent and the sample to be analysed and transfers them into the reaction cell for incubation.

2.4

ASPIRATION ARM

The aspiration arm transfers the reaction mixture from the reaction cell to the microflow cell of the photometric system for measurement.

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2.5

PROBE CLEANER

Located at the base of the sampling arm, the probe cleaner consists of a number (approximately 16) of circular filter paper strips mounted on a holder. This cleans the sampling probe before and after the aspiration and dispensing of reagents.

Figure 2 – Sampling Arm

2.6

WASHING WELL

The washing well is the place where the washing of the sampling probe takes place to avoid cross-contamination between sampling.

2.7

INCUBATOR

The incubation temperature of the reaction cell is fixed at 37°C ( ± 0,2°C ) to ensure proper incubation for all samples.

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2.8

PERISTALTIC PUMP

The peristaltic pump operates while the aspiration arm transfers the contents of the reaction cells into the micro-flow cell.

Pump operation

: by stepper motor

Nominal volume of aspiration

: from 300 to 1000 µl

Aspiration rate

: 400 µl per 1.5 sec

Figure 3 - Peristaltic Pump

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2.9

PHOTOMETRIC SYSTEM

The photometer system consists of a narrow bandwidth interference filters of 340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 576 nm, 620 nm. The other characteristics of the optical system are as follows:

Absorbance range

0.001 - 2.500 O.D.

Resolution

0.001 O.D.

Drift

< 0.003 O.D./hour

Accuracy

± 0.001 O.D.

Band-pass

± 2 nm at 340 & 405 nm; 8 nm for visible range

Light source

6V-10W halogen lamp

Detector

Silicon photodiode

Zeroing

Automatic, computer controlled Figure 4

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2.10 MICROFLOW CELL The measuring cell of the instrument is a microflow cell with the following characteristics (see figure 5):

Light path

10 mm

Cuvette body

stainless steel & quartz

Inlet tube

Teflon

Cuvette volume

50 µl

Temperature

37°C - Peltier effect

Temperature accuracy

± 0.1°C

Carry over

2% nominal for total volume of 450 - 500 µl of solution

Aspiration

computer controlled

Figure 5: Microflow Cell

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2.11 COMPUTER & SOFTWARE FEATURE Minimun requirements:



Type: IBM Compatible.



CPU: Pentium III 300 MHz - 512 Kb Cache or plus.



Memory: RAM 56 Mb or plus.



Hard Disk : 20 Gb or plus.



CD ROM 24X



Memoria Video: 4 Mb



Monitor: SVGA 15’’ color – Resolution: 1024 X 768 pixels; min. number of colors:

65.536 (16 bit) 

Rinter: Windows compatible.



Keyboard & Mouse: Standard Keyborad 101 tasti – PS2



Interface : RS 232 serial port for the Host Link - RS232 serial port or USB for Vehasys

Link. 

Software : Multitasking WINDOWS XP



Settngs: Disable all the energy saving options; Disable the screen saver.

NOTE: Even though the computers dimostrate the same technical and operative characteristics,

some of these could have different hardware installed. This could cause problems for the Vegasys software when running tests. Therefore, if the PC is bought separately/locally, it is highly recommended to test the system before proceeding with the installation at customer laboratories. Consequently, AMS denies any responsibility for the software problems that are due to buying the computer separately from the instrument.

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2.11.1 TECHNICAL & OPERATIVE FEATURES 

Description: Open, Fully Automa. It runs immunoturbidimetric, clinical chemistry

analyses using single or double reagent methodologies. 

Assy Type: End Point, Differential, Initial Rate (or Fixed Time), Kinetics and Bichromatis

methods . 

Test Selection Mode: Batch, Profile.



Throughput: Up to 2000 colorimetric test/hr.



Working Temperatue: 37° C



On Line Reagents: 18 Reagents (35 ml).



Standards & Controls: one rack for 6 standards and 2 controls.



Samples Loading: four racks for a total of 40 samples.



Minimum Reaction Volume: 450 µl



Sampling Arm: provides to aspirate reagent and sample. Automatic probe washing.



Aspiration Arm: transfers the reaction misture from the reaction cell to the microflow

cell of the photometric system of measurament. 

Diluter: Integrated module with the following specifications: 

Sample volume: 3.0 µl ÷ 99 µl (1 µl incr.)



Reagent volume 1: 3.0 µl ÷ 500 µl (1 µl incr.)



Reaction Plate: Six sectors of 19 reaction cuvettes for a ttal of 114 cuvettes.



Optic System: 

Photometer :

multi

wavelenght

photometer

with

narrow

band

interferntial filters. 

Wavelenght: 340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 578 nm and

620 nm.



Vegasys



Light source: halogen lamp 6V/10 W



Linear range: 0.001 ÷ 2.500 Abs



Resolution: 0.001 Abs



Detector : Silcon photodiode

Optical Path: 10 mm

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2.12 DIMENSION, WEIGHT & ENVIRONMENT 

Dimension 

Height: 32 cm



Depht: 45 cm



Lenght: 50 cm.



Weight: 22 Kg.



Environment: 

Temperature: 18°C ÷ 32 °C



Relative humidity: 20% ÷ 85%

2.13 INSTALLATION REQUIREMENTS 

Power requirements: 

Input Voltage: 110 ÷ 240 Vac



Input Frequency: 47 ÷ 63 Hz



180 W for the analytical unti



600 W for the work station

WARNING: A steady power supply ( ±10 %) must be provided for the instrument.

If it is not, the manufacturer highly recommends the use of:

Vegasys



UPS (Uniterruptible Power Supplì – “No Brack module”) ( 1 KW)



ELECTRONIC STABILISER

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Chapter 03- INSTALLATION

CHAPTER 03 - INSTALLATION

INDEX

3.1

UNPACKING..................................................................................................................2

3.2

INSTALLATION ............................................................................................................3

3.2.1

INSTALLATION STE SPECIFICATION .....................................................................4

3.2.2

ELECTRIC CURRENT REQUIREMENTS...................................................................4

3.2.3

CONNECTION OF THE ACCESSORIES.....................................................................5

3.2.3.1

POWER SUPPLY ...........................................................................................................5

3.2.3.2

LABELS AND SYMBOLS.............................................................................................6

3.2.4

SYMBOLS ......................................................................................................................6

3.2.5

WARRANTY ..................................................................................................................7

3.2.6

CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF WARRANTY........7

3.2.7

REGULATORY COMPLIANCE ...................................................................................8

3.2.8

LIMITATION OD USE ..................................................................................................8

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3.1 The

UNPACKING VEGASYS is

packed and delivered in a wooden crate: it contains the analyzer itself along

with its accessories. The packing has been expressly studied and designed to insure maximum protection of the contents during shipping and handling. It is therefore extremely important that the crate(s)/box be carefully examined upon delivery in order to ascertain their integrity. Special attention should be dedicated to examining the color of the “Shock Watch” glued to the crates, which must show the color ‘white’. A ‘red’ “Shock Watch” indicates that the crate(s) have experienced some sort of ‘shock’ during handling, transport and/or delivery. This fact must be noted by the courier on the delivery note, as must any and all visible external damage (for example: holes, dents, rips or tears, water marks, etc.) evident at the moment of delivery. This will simplify matters in the event of any future claims for damages.

NOTE: ONCE THE CARRIER HAS TAKEN POSSESSION OF THE SYSTEM FOR TRANSPORTATION FROM THE FACTORY, THE CARRIER ASSUMES TOTALL RESPONSABILITY UNTIL ITS DELIVERY. ALL CLAIMS FOR DAMAGE DUE TO TRANSPORTATION MUST BE FILED AGAINST CARRIER AS SOON AS THESE DAMAGES ARE NOTICED.

Upon arrival of the crate(s)/box, take out the delivery note and make sure that all the items on the packing list are included in the crates and are undamaged. Make sure the series number on the delivery note/packing list corresponds to that impressed on the plate on the right side of the instrument

Open the crate/box from the top and very carefully take out: -

the instrument and accessories;

-

the computer (if required).

MAKE SURE THAT THE UNPACKING IS CARRIED OUT BY TWO PEOPLE.

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Do not discard the delivery crate/box or the packing material until the correct functioning of the instrument has been ascertained. Remove all the items from the crate/box very carefully. Before connecting the "Vegasys" , remove the protective packing material of the sampling and aspiration arms.

Warning: in the event that it is necessary to repack any or all of the delivered item(s), the

following procedures must be carefully followed:



Reposition the protective packing material of the sampling and aspiration arm.



Tape down (using maskig tape if possible) the main cover of the instrument.



Fill the empty spaces around the accessories packed in the crate using “pluriballs” or other suitable packing material.

3.2

INSTALLATION

The VEGASYS is an instrument for professional use only and must be installed by a qualified technician who has been authorised and trained to do so. During its installation the system will be checked once again to ensure correct functioning. The persons who are required to operate the LIASYS system must have received the adequate training. This should also include the "knowhow" of the normal maintenance for the instrument. A description of the maintenance will be found in Chapter 06 of this manual. VEGASYS is a complex system, and it is therefore extremely important that it is correctly installed in order to fully guarantee fine performance. If the installation and use directions, given in this manual, are not correctly followed and/or safety indications are not respected, AMS cannot guarantee correct functioning of the instrument. Apart from this, the safety of the operator could be placed at risk .

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3.2.1

INSTALLATION STE SPECIFICATION

Ascertain that the VEGASYS system is not exposed to direct sunlight, draughts, dust or strong magnetic fields. In addition, please take note of the following conditions required for the location of the installation: USE

In covered and dry place

DEGREE OF POLLUTION

2

INSULATION CLASS

I

INSTALLATION CATEGORY

II

TEMPERATURE

Between 18°- 32°C

HUMIDITY

20% ÷ 85%

ALTITUDE

Max 3000 m Shelf or table with a minimum surface of 70 x 60 cm stable and free of vibration Leave a minimum distance of 10 cm around the instrument to permit air circulation. Make sure that the fan (situated under the reagent compartment) is not blocked by any object

LOCATION

VENTILATION

3.2.2

ELECTRIC CURRENT REQUIREMENTS

The power voltages to which the instrument is adapted are indicated on the left-hand side. It must be plugged into a plug of the correct voltage.

NOTE:

VOLTAGE

100 ÷ 230 Vac 47/63 Hz ± 10%

FUSES

5 x 20

4 Amp.

IT IS ADVISABLE TO MAINTAIN THE MAXIMUM STABILITY OF THE ELECTRICAL CURRENT IN THE LABORATORY. WHERE THIS IS NOT POSSIBLE OR ASCERTAINABLE, USE OF THE FOLLOWING SUPPLEMENTARY DEVICES IS RECOMMENDED:

ELECTRONIC STABILIZER

Used to stabilise the electric voltage in the laboratory. Any stabiliser with a power potential of at least 1KW, currently available on the market, can be used.

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NO-BREAK MODULE UPS - (Uninterrupted Power Supply)

This module provides two important functions: - stabilises the main-line power - supplies current to the instrument in case of a main-line power failure.

3.2.3

CONNECTION OF THE ACCESSORIES

3.2.3.1 POWER SUPPLY

IMMAGINE Fi . 1 – Plu DA useCAMBIARE the feeder cable su

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3.2.3.2 LABELS AND SYMBOLS

The following label is found at the rear of the instrument.

NOTE:

THE PROTECTION’S CARTER OF THE INSTRUMENT MUST NEVER BE OPENED WITHOUT HAVING FIRST SWITCHED THE INSTRUMENT OFF AND DISCONNECTED THE ELECTRICITY CABLE.

THE MAINTENANCE AND CLEANING PROCEDURES FOUND IN CHAPTER 07 OF THIS MANUAL MUST BE RESPECTED AT ALL TIMES.

R EMEMBER TO FOLLOW THE DECONTAMINATION PROCEDURE

WHEN REQUIRED BEFORE MAINTENANCE OPERATION (SEE CHAPTER 07).

3.2.4 SYMBOLS

ATTENTION: READ THE INSTRUCTIONS IN THE USER MANUAL

TERMINAL OF TOTAL MASS PROTECTION (CONDUCTOR )

BIOLOGIC HAZARD (LOCATED NEAR THE LIQUID WASTE OUTPUTS) Carefully manipulate all the consumables and the wastes produced during the analysis routines. Use appropriate protective garment. Disposal of wastes must be done in compliance with applicable regulation. It is recommended to periodically check the level in the waste container, in order to avoid overflow.

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3.2.5

WARRANTY

AMS guarantees the substitution of all defective components and/or materials for a period of time not above of twelve months starting from the date of invoicing. Saying warranty, as well as Technical Assistance, generally is intended furnished as net ex factory Rome. This warranty does not include consumable and instrument parts in contact with liquids. All components not covered by the warranty are reported in the following table. Besides guarantee does not cover damage caused by: -

improper use of the VEGASYS VEGASYS instrument (or however not not according to the Producer or Seller instructions)

-

bad transport

-

insufficient (or missing) preventive maintenance by the User

In particular any damages due to the transport must be immediately reported to the carrier when he delivers.

3.2.6

CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF WARRANTY

Description

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Type

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3.2.7

REGULATORY COMPLIANCE

The VEGASYS instrument complies with: 

3.2.8

European Directive 98/79/CE for In Vitro Diagnostic Devices

LIMITATION OD USE

The VEGASYS can not be used by blind operators because the user interface with the system requires a monitor. Furthermore the VEGASYS must be used with particular caution by color blind operators because the graphic interface displays different colors with different meaning.

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Chapter 04 – ELECTRICAL SCHEMES AND DRAWING

CHAPTER 04 - ELECTRICAL SCHEMES AND DRAWING –

INDEX

ELECTRONIC DIAGRAM VEGASYS

HYDRAULIC DIAGRAM VEGASYS

DILUTER ASSY

ANALYTICAL CONTROL BOARD

POWER SUPPLY

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CHAPTER 05 – DIAGNOSTIC PROGRAM

CHAPTER 05 - DIAGNOSTIC PROGRAMINDEX 5

DIAGNOSTIC PROGRAM ................................................. ............................................................. 3

“OPTIONS” AREA ................................................ .................................................................................. 6 “FUNCTIONS BAR” AREA...................................................... .............................................................. 6 “SETTING ARMS/ PLATE” FOLDER ................................................................................................... 7 “GENERAL” .......................................... .................................................................................................. 9 “WASHING WELL” .............................................................................................................................. 10 “REAGENT” ............................................ .............................................................................................. 11 OFFSET CORRECTIONS ................................................. .................................................................... 12 “SAMPLE” ............................................................................................................................................. 13 “CUVETTE UNDER SAMPLING ARM”.......................................................... ................................... 14 “CUVETTE UNDER THE ASPIRATING ARM” ................................................................................ 16 ”SYSTEM INFORMATION AND PARAMETERS” ........................................................................... 18 “DILUITOR” FOLDER.............................. ............................................................................................ 19 “PRIMING” ................................................. ........................................................................................... 19 “ASPIRATION AND DISPENSATION” ................................................... ........................................... 20 “DILUTER VALVE TEST” ................................................................................................................... 21 “MICROLITERS RESET” ............................................................................................................... ...... 21 ”INFORMATION” ................................................................................................................................. 21 “FOTOMETER TEST” FOLDER .................................................. ........................................................ 22 “AUTOZERO”........................................ ................................................................................................ 22 “FILTER SELECTION AND START READING” .............................................................................. 23 “READING FILTERS” .......................................................................................................................... 24 “DRAWING”.......................................................................................................................................... 24 “OPTICAL DENSITY” Folder............................................................................................................... 25 “ASPIRATION” ............................................... ...................................................................................... 25 Vegasys

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“SELECT FILTER & START READING” ........................................................................................... 26 “DRAWING”.......................................................................................................................................... 27 ”INFORMATION” ................................................................................................................................. 27 “PERISTALTIC PUMP” FOLDER ....................................................................................................... 28 “CALIBRATION” ............................................... ................................................................................... 28 “ASPIRATION ANS ASPIRATION STEPS” ......................................................... .............................. 29 “SOLENOID VALVE TEST” ................................................................................................................ 29 “CANCELING THE PERISTALTIC PUMP STEPS”........................................................................ ... 29 ”INFORMATION” SECTON......................................... ........................................................................ 30 “CUVETTE TEMPERATURE”............................................................................................................. 30 ”INFORMATION” ................................................................................................................................. 32 “PLATE TEMPERATURE”................................... .......................................................... ...................... 32 SEZIONE ”INFORMAZIONE” .................................................. ................................................... ........ 33 MASCHERA “CICLO DI LAVORO” ................................................... ................................................ 34 ”INFORMATION” ................................................................................................................................. 38 MASCHERA “VARIE”............................... ........................................................................................... 38 MASCHERA “DOWNLOAD” .......................................................... .................................................... 41

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5

DIAGNOSTIC PROGRAM

The diagnostic program enables the operator to perform a complete check for each of the Vegasys’ module functions. This program has a folder structure, with each folder containing functions pertaining to the specific module. To launch the program, the operator has to click with the left side of the mouse, on the Diagnostic area located on the lower right side of the screen of the System Monitor (Fig.1).

The Liasys must be in the stand-by state to access this area.

Fig. 1

Click Here

Click on the “Diagnostic ” area, and a window with “Password Required ” will appear. It is necessary to type in a secret access code and confirm by clicking on “OK ”.

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This operation can be canceled by clicking on “Exit” .

After the password is given, the following screen appears: (Fig. 2):

Fig. 2

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The following information for the options currently b eing used is given on this screen:

   

Icon Symbol for the Language Selected Master Hardware Version Serial Port Firmware Version of the Control Board

The following options are also available:

Closes the screen and returns to the System Monitor.

Gives access to the Main Diagnostic Window.

Enables the operator to select the language (Italian, English, French, German,…)

The diagnostic program is subdivided into two distinct areas: 

Options



Status Bar

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Fig. 3

“OPTIONS” AREA

Closes the “Diagnostic” program and returns to the System Monitor.

“FUNCTIONS BAR” AREA

The following is information pertaining to each individual function.

Arm/Plate Calibration:

Checks if the Sampling/Aspiration Arm and the Reaction Plate are functioning correctly and performs mechanical calibration.

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Diluitor:

Checks the performance of the Diluter module;

Photometer Lamp Change:

Checks the performance of the Photometer Lamp;

Optic Density:

Measures the absorbance of the solution in the Flow Cell;

Peristaltic Pump:

To calibrate the aspiration of the Peristaltic Pump;

Temperatura Cuvetta:

To calibrate the temperature of the Flow Cell;

Plate Temperature:

To calibrate the temperature of the Reaction Plate;

Work Cycle (Work Load):

To prepare and/or read one or more samples;

Various:

To reset the reaction sectors, print the parameters set for the various modules and to select a serial port from those identified by the Master Hardware. To download and update the Firmware of the Control

Download:

Board.

“SETTING ARMS/ PLATE” FOLDER Warning: Only qualified technicians are allowed access to the folder “Setting Arms/Plate”. In

order to access the window it is necessary to insert the Operator Name and Password.

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To confirm the information given, click on

The button Calibration” .

enables the operator to cancel the operation of accessing the window “Arm/Plate

The above window is subdivided into the following area:       

Vegasys

“General” “Washing Well” “Reagent” “Samples” “Cuvette” (which is different from the sampling/aspirating arm) “NO3 and Free Chassis” “Information and System Parameters”

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“GENERAL”

This area has the following commands:

Performs the resetting of the general system.

Vertically resets the sampling and aspirating arm

Disengages the motors to enable the Sampling Arm and Reaction Plate to be manually adjusted. Note: To engage the motors again just select “General Reset”.

Gives information on the screen regarding some functions of the folder presently open.

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“WASHING WELL”

This area has the following commands:

Brings the sampling arm to the home position (washing well). Lowers the sampling probe of a number of steps specified in the small window under the arrow. By selecting the command “Washing Well”, the following buttons appear automatically which enable the operator to define the number of steps to go down.

Increases the number of steps down of the sampling probe by increments of “1”; Increases the number of steps down of the sampling probe by increments of “5”; Decreases the number of steps down of the sampling probe by increments of “1”; Decreases the number of steps down of the sampling probe by increments of “5”;

Once the number of steps for the descent are defined, by choosing the command “Down”, the sampling probe goes down to the washing well using the number of steps programmed (in the example, 45 steps).

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“REAGENT”

The following commands are available:

Brings the sampling arm in the reagent position n° 1 if the option

is selected (or in reagent position n° 10

if the option

is selected).

This command lowers the sampling probe down to the reagent container, using the number of steps indicated in the small window under the arrow. By selecting this command the following arrows automatically appear which enable the operator to define the number of steps down. Increases the number of steps down of the sampling probe by increments of “1”; Increases the number of steps down of the sampling probe by increments of “5”; Decreases the number of steps down of the sampling probe by increments of “1”; Decreases the number of steps down of the sampling probe by increments of “5”;

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This command lowers the sampling probe down to the reagent container, using the number of steps indicated in the small window under the arrow. By selecting this command the following arrows automatically appear which enable the operator to define the number of steps down ( in the example, 45 steps).

The command “Up” makes the sampling probe rise up, using the number of steps indicated in the small window under the arrow (in the example, 40 steps).

Increases the number of steps up of the sampling probe by increments of “1”; Increases the number of steps up of the sampling probe by increments of “5”; Decreases the number of steps up of the sampling probe by increments of “1”; Decreases the number of steps up of the sampling probe by increments of “5”;. OFFSET CORRECTIONS

By positioning the Sampling Arm in the n° 1 postion of the reagent, it is possibile to improve the sampling probe’s alignment a s follows: 



Moves the sampling arm (in a clockwise/counter-clockwise movement) in regards to the reagent container opening.

Moves the reaction plate (in a clockwise/counter-clockwise movement) in regards to the sampling probe.

Moves the sampling arm clockwise 1 step at a time; Moves the sampling arm clockwise 5 steps at a time; Moves the sampling arm counter-clockwise 1 step at a time; Moves the sampling arm counter-clockwise 5 steps at a time.

The same rules apply to the arrows for moving the Reaction Plate with respect to the Sampling Arm.

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“SAMPLE”


Brings the Sampling Arm to sample cup n° 1.

Brings the sampling probe down to the sample cup n° 1 using the number of steps programmed. By selecting the command “Sample”, the following arrows automatically appear which enable the operator to define the number of steps down.

Increases the number of steps down of the sampling probe by increments of “1”; Increases the number of steps down of the sampling probe by increments of “5”; Decreases the number of steps down of the sampling probe by increments of “1”; Decreases the number of steps down of the sampling probe by increments of “5”.

Once the steps down have been programmed, choose the “Down” command and the sampling probe will automatically go down to the sample cup n° 1, using the number of steps programmed (in the example, 45 steps).

The “Up” command brings the sampling probe up using the number of steps programmed (in the example, 40 steps).

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Increases the number of steps up of the sampling probe by increments of “1”; Increases the number of steps up of the sampling probe by increments of “5”; Decreases the number of steps up of the sampling probe by increments of “1”; Decreases the number of steps up of the sampling probe by increments of “5”.

OFFSET CORRECTIONS

The probe’s alignment can be adjusted when the sampling arm is positioned in the sample cup n° 1 position as follows: Moves the sampling arm steps (clockwise/counterclockwise) with respect to the sampling cuvette opening. 



Moves the reaction plate n steps (clockwise/counterclockwise) with respect to the sampling probe.



“CUVETTE UNDER SAMPLING ARM”

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Allows to bring the first cuvette of the Reaction Plate under the sampling arm.

Once the steps down have been programmed, choose the “Down” command; the sampling probe will automatically go down to the Reaction Plate cuvette n° 1, using the number of steps programmed. By selecting the command “Cuvette-Sampling ”, the following arrows automatically appear which enable the operator to define the number of steps down.

Increases the number of steps down of the sampling probe by increments of “1”; Increases the number of steps down of the sampling probe by increments of “5”; Decreases the number of steps down of the sampling probe by increments of “1”; Decreases the number of steps down of the sampling probe by increments of “5”.

Once the steps down have been programmed, choose the “Down” command; the sampling probe will automatically go down to the reaction cuvette n° 1, using the number of steps programmed (in the example, 45 steps).

OFFSET CORRECTIONS

The probe’s alignment can be adjusted when the sampling arm is positioned in the reaction cuvette’s n° 1 position as follows: 



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Moves the sampling arm (clockwise/counterclockwise) with respect to the sampling cuvette opening.

Moves the reaction plate (clockwise/counterclockwise) with respect to the sampling probe.

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“CUVETTE UNDER THE ASPIRATING ARM”

1° “Down” Command

2° “Down” Command


Moves the reaction cuvette n° “1” under the “Aspirating Arm” if option option

is selected (or the reaction cuvette n° 58 if the is selected).

The first “Down” command enables the operator to define the first descent of the aspiration probe to the Reaction Plates cuvette. The depth of the first descent is useful for aspirating liquid for the reaction (together with the peristaltic pump) which is useful for emptying an d rinsing the flow cell. This first descent depends on the volume which remains in the cuvette of the Reaction Plate which should not be less than …….. (volume which is useful for the next aspiration).

The second “Down” command enables the operator to define the number of steps down for the second descent of the aspiration probe into the Reaction Plate cuvette. This second descent should aspirate the minimum quantity of the reaction liquid necessary for a reading.

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By selecting the command “Cuvette-Aspiration ”, the following arrows automatically appear which enable the operator to define the number of steps down. Increases the number of steps down of the aspiration probe by increments of “1”; Increases the number of steps down of the aspiration probe by increments of “5”; Decreases the number of steps down of the aspiration probe by increments of “1”; Decreases the number of steps down of the aspiration probe by increments of “5”.

OFFSET CORRECTION

It is possibile to improve the alignment of the aspiration probe’s on the reaction cuvette by doing the following: 

Move the reaction plate n steps (clockwise/counterclockwise) with respect to the aspiration probe.

Moves the Reaction Plate clockwise 1 step at a time;; Moves the Reaction Plate clockwise 5 steps at a time;; Moves the Reaction Plate counter-clockwise 1 step at a time; Moves the Reaction Plate counter-clockwise 5 steps at a time.

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”SYSTEM INFORMATION AND PARAMETERS”

The following tables are available:

TableA This table shows the results (under the heading “Status”) of various actions which have been run by the technician/operator in the “Diagnostic” program.

Table B This table shows all the System settings under the heading “Value”.

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“DILUITOR” FOLDER

The above folder is divided into five sections:     

“Priming” “Aspiration and Dispensation” “Diluter Valve Test” “Zeroing Microliters” “Information”

“PRIMING”

This section has the command “PRIME” and the field “Cycles” (1..9)”.

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Enables the operator to rinse the sampling hydraulic circuit.

To set the number of cycles to be run. (priming) The minimum number of cycles to be run is 1 and the maximum is 9.

The operations performed during a “PRIME” cycle are the following:



the diluter aspirates 1000 µl of liquid from the loading bottle



the aspirated liquid is dispensed by the sampling probe into the washing well and transferred by the peristaltic pump to the waste bottle.

“ASPIRATION AND DISPENSATION”

This section has the following commands and fields:

To aspirate the number of microliters previously programmed in the field “µl”

To set the number of microliters of liquid that the sampling probe should aspirate. To conferm the number of microliters programmed, push “Enter” (from the keyboard). To dispense the number of microliters previously aspirated.

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“DILUTER VALVE TEST”

Allows to test the proper functioning of the diluter valve. By selecting this command the solenoid valve is automatically turned ON/OFF.

“MICROLITERS RESET”

This section shows the number of micro-liters aspirated by the diluter. By selecting the command “Microliters Reset ” the count of the microliters aspirated are zeroed and visible in the field “Total L”.

Shows the hydraulic the sampling circuit.

”INFORMATION” This table shows the results (under the heading “Status”) of various actions which have been run by the technician/operator in the “Diagnostic” program.


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“FOTOMETER TEST” FOLDER

The above folder is divided into the following sections: 

Autozero



Filter Selection and Start reading



Reading Filter



Drawing



Information

“AUTOZERO”

Performs the automatic autozero of the fotometer, where the Vegasys will sample and aspirate distilled water twice (the liquid located in the loading container). The values read are then reported on the table and graph (in millivolt and counts) for each filter.

Reports and Prints the last autozero.

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“FILTER SELECTION AND START READING”

This section has the field “Select Filter ” and the command “Start Reading ”.

Allows the user to select a filter from a pull down menu list

Allows to start the reading at the filter selected. It turns to “ STOP” when select.

Warning: make sure the micro-flow cell is filled of Rinse solution.

Allows to stop the reading.

By selecting “Start Reading” the following appears on the screen:



a graph showing the che mostra l’andamento nel tempo della tensione in “mv” for the filter selected, rilevata alla uscita dell’amplificatore fotometrico.



Vegasys

A table which shows in “mv” and “Counts” for the filter selected:

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“READING FILTERS”

This command automatically performs the reading of the liquid present in the flow cell for each filter. It is important to make sure that the flow cell is full of liquid.

By clicking on the option “Filters Reaging ”, the following information appears on the screen: 

una tabella che riporta per ciascun filtro i valori letti in “ mv (Ultimo) ” e “Counts (Ultimo) ”



un grafico che mostra in funzione del numero del filtro il valore della tensione in “mv” rilevata alla uscita dell’amplificatore fotometrico.

“DRAWING”

Shows the “Fotometric System” drawing.

”INFORMATION”

This table shows the results (under the heading “Status”) of various actions which have been run by the technician/operator in the “Diagnostic” program.


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“OPTICAL DENSITY” Folder

This folder is subdivided into two sections: 

“Aspiration ”



“Select Filter & Start Reading ”



“Drawing”



“Info”

“ASPIRATION”

This section has the command “Aspiration ”and the field “Aspiration Steps ”.

Aspirates liquid from the aspiration probe (this is done by removing the aspiration probe from the aspiration arm and dip it into the test solution). This field enables the operator to program the number of steps of the peristaltic pump in order to improve the aspiration of the liquid. * Minimum number of steps = 1; Max = 4000

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“SELECT FILTER & START READING”

This section has the field “Select Filter ” and the command “Start Reading ”.

Allows the user to select a filter from a pull down menu list

Allows to start the reading at the filter selected. It turns to “STOP” when select.

Warning: make sure the micro-flow cell is filled of test solution.

Allows to stop the reading.

By selecting “Start Reading” the following appears on the screen:



una tabella che riporta per ciascun filtro i valori letti in “mv”, “Counts ” e “D.O.” (densità ottica)



Vegasys

un grafico che riporta l’andamento nel tempo della densità ottica.

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“DRAWING”

Shows the “Fotometer System” drawing.

”INFORMATION”

This table shows the results (under the heading “Status”) of various actions which have been run by the technician/operator in the “Diagnostic” program.


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“PERISTALTIC PUMP” FOLDER

This folder is subdivided into five sections:



“Calibration ”



“Aspirate and Aspiration Steps ”



“Solenoid Valve”



“Canceling Peristaltic Pump Steps”



“Information ”

“CALIBRATION”

This section allows to perform an automatic verification and adjustment of the peristaltic pump aspiration.

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Allows to carry out the calibration procedure.

These fields enables the operator to program the number of aspiration steps for the peristaltic pump during calibration (“Final Aspirat.”) and the number of calibration c cles to run “C cles 1..9 ” .

“ASPIRATION ANS ASPIRATION STEPS”

This section has two options: “Aspiration ” and the field “Aspiration Steps ”. Aspirates liquid away from the aspiration probe.

This field enables the operator to program the number of steps of the peristaltic pump in order to improve the aspiration of the liquid. * Minimum number of steps =1; Max= 4000

“SOLENOID VALVE TEST”

To test the proper functioning of the diluter valve. This option automatically switches the solenoid valve “ON/OFF”.

“CANCELING THE PERISTALTIC PUMP STEPS”

This section has two options: “Canceling Steps ” and the field “Aspiration Steps ”.

This option enables the operator to cancel the total number of Peristatic Pump Steps.

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This field shows the total number of steps run by the Peristaltic Pump.

In questa maschera è inoltre presente il pulsante

che permette di visualizzare lo

schema fotometrico.

”INFORMATION” SECTON

In questa sezione è presenta una tabella che visualizza nel campo “Stato” le risposte alle azioni esercitate dal tecnico nelle diverse maschere presenti nel programma di Diagnostica.

La selezione di questo pulsante permette di avere informazioni sul significato di alcune funzionalità presenti nella maschera in esame.

“CUVETTE TEMPERATURE” Warning: l’accesso alla maschera “Temperatura Cuvetta” è permesso al solo personale tecnico

specializzato. Per accedere alla maschera è necessario inserire il Nome Operatore e Password.

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Per confermare i dati inseriti , selezionare il pulsante

Click on

to cancel the operation to access the window “Cuvette Temperature ”.

The above window can be accessed only after a password has been given. It is possible to perform the calibration procedure of the flow cell using a sonda termica (Kit P/N: ……..) as illustrated in the chapter “Settings and Calibration”. Starts the calibration process (8 points) of the Flow Cell Temperature.

Prints the data of the calibration curve and graphically visualizes the temperature in funzione dei Counts.

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Starts the reading process of the flow cell’s temperature, and updates the graph as new readings are taken .

”INFORMATION”



“PLATE TEMPERATURE” Attenzione: l’accesso alla maschera “Temperatura Piatto” è permesso al solo personale tecnico


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Il pulsante

consente di annullare l’operazione di accesso alla maschera “Temperatura Piatto ”.

La maschera in esame è accessibile solo attraverso l’inserimento di una password. In questa sezione è possibile eseguire la procedura di calibrazione della cella a flusso mediante l’utilizzo di una sonda termica (Kit P/N: ……..) co me illustrato al capitolo “Settaggi e tarature”.

La selezione di questo pulsante permette di avviare la procedura di calibrazione della temperatura del Piatto di Reazione. Questo pulsante permette di stampare i dati della curva di calibrazione (8 punti) e di visualizzare il grafico della Temperatura in funzione dei Counts. Questo pulsante permette di avviare il processo di lettura della temperatura nel Piatto di Reazione e di visualizzarne l’andamento grafico nel tempo. SEZIONE ”INFORMAZIONE”


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MASCHERA “CICLO DI LAVORO”

This window has the option to run one or more test cycles. This window has the following commands: Starts the work cycle/s.

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This area enables the operator to define the following operations: 

Preparation = Prepares the reaction cell with only Regent, or

with Reagent + Sample; 

Reading = aspiration and consequent reading of the prepared

mixture in the reading cell 

Sample.1 = programs the components of the mixture for the

reading; it is possibile to select a reading of the Reagent alone, or also include the Sample (only one sample cuvette which must be located in the first position of the sample secton); 

Sample. 1..n = programs the components of the mixture for the

reading; it is possibile to select a reading of the Reagent alone, or also include the Sample. If this is the case, the number o cuvettes to be positioned in order in the sampling area starting with sample n° 1, coincides with the number of cycles programmed.

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This areas has the following fields: 

N° Cycles= to program the number of work cycles;



Vol. Reag. = to define the quantity (µl) of the Reagent to be

aspirated 

Vol. Camp. = to define the quantity (µl) of the Sample to be

aspirated; 

Incubation = to program the amount of time for incubation (sec)

of the mixture (Reagent or, Reagent + Sample) in the reaction cell; 

N° Readings = to program the number of readings to run at the

end of the incubation period of the mixture in the flow cell; 

Min (mAbs) = sets the parameter (in mAbs) for the minimum

value of the reading; 

Max. (mAbs) = sets the paramenter (in mAbs) for the maximum

value of the reading; 

Section = Indicates the first reaction section ready for use;



Cuvette = Indicates the first reaction cell available for the above

mentioned section. If there is no cuvette available, the symbol “?” appears in the field Section and in the field cuvette, with the background flashing white and green. 

Filter = to select the filter to perform the Reading..

Note: The Reagent container must be in the first position (“ R1”) of the Reaction Plate.

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Setting Examples

The system aspirates Reagent located in the first position of the Reaction Plate and dispenses in the first available reaction c uvette.

The system aspirates Reagent located in the first position of the Reaction Plate; at the end of the incubation period the Reagent is aspirated from the flow cell, and runs the number of readings programmed in the field “N° letture”. The system aspirates “Reagent + Sample”; at the end of the incubation period the flow cell is aspirated and runs the number of readings programmed in the field “N° letture”.

The system aspirates “Reagent + Sample”; at the end of the incubation period the flow cell in aspirated and runs the number of readings programmed in the field “N° letture”. The number of Samples examined (trattati) is equal to the number of cycles programmed in the field “N°Cicli”. At the end of the sampling process a table automatically appears, bearing the data from the reading process and a graph which shows how the reaction progresses over time. The table has the following information: N: indicates the cycle number; D.O: indicates the value of the last reading (espressed in assorbance); Media: indicates the mean value of the readings (expressed in absorbance); Min.: indicates the minimum value of the reading (expressed in absorbance); Max.: indicates the maximum value of the reading (expressed in absorbance) CV%: indicates the coefficient of the percent of variation; mv: indicates the millivolt pertaining to the reading; Pnt/Tmp : indicates the counts pertaining to the NTC and the corresponding temperature in the flow

cell. During the reading process a a graph automatically appears which shows the progress in time of the optical density. If the number of cycles programmed is greater than 1, at the end of the reading process a graph automatically appears which shows the optical density for every reaction. Vegasys

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”INFORMATION”



MASCHERA “VARIE”

This window has the following commands:: 

Zeroing the reaction sections (SR1, SR2,SR3,SR4,SR5,SR6)



To print the system parameters.



To define a serial port porta seriale to be connect the Vegasys and the PC.

Use this command to visualize the sectors and pertaining reaction cuvettes used for analysis. (see Table 1).

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Tabella 1

The reaction cuvettes used for the sample routine of the Vegasys are indicated in red ( in the example, 6 reaction cuvettes have been used in section SR1). The reaction cuvette which have not been used are indicated in green.

To zero a section, click with the mouse on the heading (vedi Fig. 1). Once a section has been zeroed, the first reaction cuvette in that section becomes automatically available. Section Heading

Fig. 1

Important: It is possible to change the status of each single cuvette by clicking on the same.

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This field indicates the first reaction cuvette available for sampling (cuvette n° 5). SR1/C5 = indicates the sector and location available for sampling. Important : The symbol “?” indicates that there are no reaction cuvettes available for the sampling process.

This option prints the system parameters and saves the results in the micro (Aspiration Pump, Step Sampling Probe Descent to the Reagent, Step Sampling Probe Ascent to the Reagent, etc..).

Questo pulsante permette di definire una porta seriale tra quelle automaticamente rilevate dal Vegasys.

”INFORMATION”



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MASCHERA “DOWNLOAD” Warning: l’accesso alla maschera “Temperatura Download” è permesso al solo personale tecnico



Il pulsante

consente di annullare l’operazione di accesso alla maschera “Download ”.

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This window enables the operator to Download the control board’s firmware.

There are two options: Download with Default parameters to be used for the first/initial progamming of the Control Board (Firmware???).

Download with automatic saving of the system parameters to be used for updating the Control Board Firmware. The firmware first reads the status of the Control Board Firmare parameters, and then at the end of the Download, transfers once again the previously read parameters.???? In this manner, the settings made prior to the updating are saved.

In questa tabella si visualizzano nel campo “Stato” le risposte alle azioni esercitate dal tecnico nelle diverse maschere presenti nel programma di Diagnostica.

Questa tabella visualizza nel campo “Valore” tutte le impostazione del sistema.

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Chapter 06 - Maintenance

CHAPTER 06 - MAINTENANCE

INDEX

MAINTENANCE ................................................................................................................................ 2 PREVENTIVE MAINTENANCE....................................................................................................... 2 LIST OF PARTS SUBJECT TO WEAR AND USAGE..................................................................... 3 EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE .............................. 4 WASH SOLUTION BOTTLE - CLEANING..................................................................................... 4 WASH SOLUTION BOTTLE CLEANING PROCEDURE .............................................................. 4 PROBE CLEANER PAD – REPLACING PROCEDURE ................................................................. 5 HYDRAULIC CIRCUIT WASHING ................................................................................................. 6 HYDRAULIC CIRCUIT CLEANING PROCEDURE ....................................................................... 6 CHANGING THE PERISTALTIC PUMP TUBE .............................................................................. 7 PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE ........................................... 7 CHANGING THE PHOTOMETER LAMP...................................................................................... 10 PROCEDURE FOR REPLACING THE PHOTOMETER LAMP................................................... 10 CHANGING THE TUBES KIT ........................................................................................................ 12 PROCEDURE FOR REPLACING THE TUBES KIT...................................................................... 12 LIST OF THOSE MAINTENANCE PROCEDURES THAT CAN BE PERFORMED BY THE USER AND/OR THE MAINTENANCA TECHNICAN ............................................................................................................... 13

DECONTAMINATION PROCEDURE............................................................................................ 14

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MAINTENANCE

This chapter contains all those routine operations, which concern instrument maintenance. Said procedures, listed and described below, should be carefully and scrupulously followed in order to guarantee the manufacturer’s specifications and the perfect working order of the instrument over time. PREVENTIVE MAINTENANCE MAINTENANCE SCHEDULE

Table A, illustrated below, lists all those procedures to be carried out by the user/operator and the relative frequency schedule. Strict adherence to said schedule will guarantee the optimal operative efficiency of the instrument. This program does not include the manual cleaning of reagent containers. Cleaning and decision about eventual substitution of reagent containers must be made in conformity with good laboratory practice. MAINTENANCE SCHEDULE FREQUENCY

PROCEDURE

NOTES

DAILY – Before launching “Start Work” DAILY – Before launching “Start Work” DAILY – Before launching “Start Work”

Check the levels of the wash solutions. Check the levels of Reagents, Standards and Controls. Check the levels of the Waste Bottles and, if necessary, empty it.

DAILY – Before launching “Start Work”

Check the probe cleaner pad.

DAILY – After Shutdown

Clean the Sampling and Aspiration Probe using either paper toweling or gauze

FREQUENCY

PROCEDURE

NOTES

200-300 TEST

Change the probe cleaner pad.

EVERY WEEK

Clean the Sampling and Aspiration Probe (with sodium hypoclorite).

EVERY TWO WEEKS

Clean the wash solution bottle.

ONCE A MONTH

Clean the Hydraulic Circuit

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See “External Sampling/Aspiration Probe Cleaning Procedure”

Rev.00

See “Probe Cleaner Pad – Replacing Procedure” See “External Sampling/Aspiration Probe Cleaning Procedure” (see the Note of the procedure) See “Wash Solution Bottle Cleaning Procedure”. See “Hydraulic Circuit Cleaning Procedure”.

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EVERY SIX MONTHS

Change the Peristaltic Pump Tube

EVERY SIX MONTHS

Check all the tubes.

ONCE A YEAR

Change all the tubes (Tubes Kit)

EVERY 2000 HOURS

Change the Photometer lamp



See “Procedure For Replacing the Peristaltic Pump Tube”.

See “Procedure For Replacing The Tubes Kit”. See “Procedure For Replacing The Photometer Lamp”.

N. B.: the above-described maintenance schedule refers to that situation in which the workload of the Analyzer is approximately 500 tests per day. The interval frequency may vary according to the individual instrument’s daily workload.

LIST OF PARTS SUBJECT TO WEAR AND USAGE Description

Type

Quantity

Code

Racks for Reaction Cells Rack for Sample Cups (positions 1-10) Rack for Sample Cups (positions 11-20) Rack for Sample Cups (positions 21-30) Rack for Sample Cups (positions 31-40) Rack for Standard & Control Cups Packs of Sample Cups of 0.8 ml Reagent Containers (XX PCS) Plastic Bottles (H2O/Rinse & Waste) Sampling Probe Aspiration Probe Tubing Kit Complete Peristaltic Pump Tube Probe Cleaner Pad Photometer Lamp Kit for E.V.Diluter connection Diluter E.V. Peristaltic Pump E.V. Wash Solution Diluter Head Teflon Fitting Diluter Fitting

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EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE

1)

Turn off the Analyzer.

2)

Loosen the knob which blocks the sampling/aspiration probe to the sampling/aspiration arm.

3)

Remove the sampling/aspiration probe from the support frame.

4)

Use only lint-free paper toweling or gauze.

5)

Dampen the gauze or paper toweling with distilled water and clean outside of the sampling/aspiration probe. Wipe the probe from the top downwards only! This is to avoid that any bits of cloth, paper or lint fibers accidentally enter the probe itself.

6)

Insert sampling/aspiration probe in the support frame of the sampling/aspiration arm.

7)

Tighten the knob which blocks the sampling/aspiration probe in the sampling/aspiration arm.

Note: The manufacturer suggest that once weekly the above – described cleaning procedure be performed on a weekly basis using, instead of only simple distilled water, a 5% sodium hypochlorite solution to dampen the gauze and then be repeated using distilled water.

WASH SOLUTION BOTTLE - CLEANING

During normal use and over time, mold and dust can build up inside the wash solution bottles. For this reason, it is extremely important that they be periodically washed. This cleaning must be thorough and meticulous in order to insure that every trace of mold or residue be removed. How often the bottle must be cleaned depends on their use and on the quality of the distilled water used in that particular laboratory. However, the manufacturer recommends thorough washing at least once every two weeks. It is extremely important that the user not underestimate the risks associated with mold and dust particles. They are to be regarded as a serious hazard as they can be the cause of instrument malfunction.

WASH SOLUTION BOTTLE CLEANING PROCEDURE

1)

Turn off the Analyzer;

2)

Pull the aspiration cannula out from the bottle;

3)

Take the cap off the bottle and empty it;

4)

Fill the bottle with 5 % sodium hypochlorite solution;

5)

Clean the inside of each bottle using a bottlebrush in order to remove all traces of mold and/or residue;

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6)

Leave the sodium hypochlorite solution stand in the bottles for at least ten minutes;

7)

Empty the bottle, rinse it repeatedly and well with tap water, and then twice more using distilled water;

8)

Dry the bottle;

9)

Fill the bottle with proper solution (Rinse);

10) Close the bottle; 11) Reconnect the aspiration cannula; 12) Carry out eight “PRIME” cycles checking that the sampling hydraulic circuit is filled of liquid. PROBE CLEANER PAD – REPLACING PROCEDURE

Figure 1 Located at the base of the sampling arm, the probe cleaner consists of a number (approximately 16) of circular paper strips mounted on a holder. This cleans the sampling probe before and after the aspiration and dispensing of reagents.

1.


2.

Remove the probe cleaner pad from its housing (located at the base of the sampling arm);

3.

Replace the used probe cleaner pad with a new one;

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HYDRAULIC CIRCUIT WASHING

During normal use and over time, mold and dust can build up inside the wash bottle and can have a negative effect on the hydraulic circuit, compromising the correct functioning of the system. For this reason, it is extremely important that the hydraulic circuit be periodically washed. Said cleaning must be thorough and meticulous in order to assure that every trace of mold or residue be removed. How often the hydraulic circuit must be washed depends on the operating conditions/environment of the single instrument and the quality of the distilled water used in that particular laboratory. The manufacturer recommends thorough washing at least once a month. It is extremely important that the user not underestimate the risks associated with mold and dust particles. They are to be regarded as a serious hazard as they can be the cause of instrument malfunction. The hydraulic circuit input cannula is located inside the wash solution bottle.

HYDRAULIC CIRCUIT CLEANING PROCEDURE

1)

Turn on the Analyzer;

2)

Prepare a bottle containing 500 ml of a 5% sodium hypochlorite solution;

3)

Insert the aspiration cannula, located inside the wash solution bottle, into the bottle containing the sodium hypochlorite solution;

4)

Have the instrument carry out eight “PRIME” cycles;

5)

Have the instrument carry out three ‘Autozero’ cycles;

6)

Pull the aspiration cannula out from the bottle containing the sodium hypochlorite solution;

7)

Clean and dry the aspiration cannula. Insert the aspiration cannula into a bottle containing distilled water;

8)

Have the instrument carry out five “PRIME” cycles;

9)

Have the instrument carry out three ‘Autozero’ cycles;

10)

Insert the aspiration cannula back into the respective bottle (wash solution bottle). Said bottle should have, in the meantime, been cleaned and filled with a fresh supply of the required solution;

11)

Have the instrument carry out a “PRIME” cycle and then an “Autozero ” cycle to check the system efficiency.

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CHANGING THE PERISTALTIC PUMP TUBE

The manufacturer recommends that the Peristaltic Pump tube be replaced every six months. Said frequency may vary depending on the workload of the individual laboratory. The quality and reliability of these tubes is fundamental to a correct emptying of the micro-flow cell.

PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE

The diameter and the length of the tubings for the flow system are very critical for the proper functioning of Vegasys. Please make sure that the tubings are in accordance with the manufacturer's specifications. 1.

Switch off the instrument and unplug the power cord;

2.

Loosen knob “A” and move block “B” downwards (see Figure 2);

3.

Remove the old tube from the connection;

4.

Replace the new tube and connect it again to the junction;

5.

Push block “B” up to block “D” and tighten knob “A”;

6.

Check the aspiration volume using the "Start Calibration" function of the Vegasys software (Chapter 06 – Calibration Pump Procedure).

7.

Have the instrument carry out an ‘Autozero ’ cycle as functional check.

Figure 2

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CALIBRATION PUMP PROCEDURE

This procedure enables the user to set up the volume of the reaction mixture that is aspirated by the peristaltic pump from the reaction cell to the microflow cell via the aspiration tube.

Warning: before activating this procedure, one reagent container with Rinse Solution must be placed on the analytical plate at reagent position R1.

1. Launch “Diagnostic” program and wait for an instrument general reset.; 2. In order to view the various elements contained under “Diagnostic” program, insert the valid Password within the provided field. Click on “ OK ”; 3. Press “Peristaltic Pump” button; 4. Press “Start Calibration” button; the instrument carry out a microflow cell wash cycle; 5. Make sure that the tubes (leading to and from the microflow cell) fill up of liquid, as shown in the below figure (see the bold black lines).

(B)

(A)

Figure 3

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6. Referring to the diagram above, where the solid bold, black lines indicate the silicon tubes leading to and from the microflow cell. If in the area B the liquid goes too much beyond the bold area indicated meaning that there is too much liquid in area B, and if in area A the liquid does not completely fill the bold, black line, it is necessary to decrease the steps in the “Final Aspiration” field. Push the “ Start Calibration” button again and check the liquid level in the tubes. On the other hand, if the liquid in area A is too much and in area B there is not enough, increase the number of steps in the “ Final Aspiration” field. Push the “Start Calibration” button again and check the liquid level in the tubes.

Nota: If the adjustment cannot be made by adjusting the software, it is necessary to adjust the

pressure between the silicon tube and the peristaltic pump wheel through the keep plate of the peristaltic pump. WASHING MICRO FLOW CELL - PROCEDURE

This procedure enables the automatic washing of the microflow cell with a 5% sodium hypochlorite solution. Before activating this procedure, put some 5% sodium hypochlorite solution into one of the reagent container. 1) Put some 5% sodium hypochlorite solution into one of the reagent container; 2) Place the reagent container with 5% sodium hypochlorite solution in position R1 of the Reaction Plate;. 3) Ensure that the reaction rack SR1 is available in the analytical plate; 4) Launch the “Diagnostic” program; 5) In order to view the various elements contained under “Diagnostic” program, insert the valid Password within the provided field. Click on “ OK”; wait for the instrument general reset; 6) Select “Diagnostic” button; 7) Select “Work Cycle” button; 8) Press “Start Cycle”; the sampling probe takes the sodium hypochlorite solution from the reagent container and dispense it into the first free reaction cell. The aspiration probe will then take the solution in the reaction cell and the solution flows through the microflow cell; 9) Leave the sodium hypochlorite solution stand in the microflow cell for at least 15 minutes; 10) Remove the reagent container with sodium hypochlorite solution from “ R1” position of the Reaction Plate;

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11) Place a reagent container with Distilled Water in position “R1” of the Reaction Plate; 12) Set the “N° Cycles” equal to 5; 13) Press “Start Cycle”.

The manufacturer suggests to carry out the below-described washing microflow cell procedure al leas once per week. CHANGING THE PHOTOMETER LAMP

The manufacturer suggests that the lamp be replaced after approximately 2000 hours of use. The lamp is mounted on the photometer, which is located on the left-hand side of the reaction plate. PROCEDURE FOR REPLACING THE PHOTOMETER LAMP WARNING! DO NOT TOUCH THE GLASS PART OF THE LAMP WITH YOUR FINGERS!

IF NECESSARY, USE A CLEAN CLOTH TO REMOVE DUST, OR ALCOHOL TO REMOVE MORE STUBBORN DIRT.

Figure 4 1.


2.

Unscrew the screw which holds the white cover panel of the photometer. Remove the white cover panel.

3.

Unthread the terminals of lamp. (designated as "A" in Figure 4).

4.

Unscrew the block of lamp. (designated as "B" in Figure 4) and remove the lamp;

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5.

Replace the old lamp with a new one making sure that the pin is correctly inserted in the alignment hole;

6.

Make sure that the lamp regulator voltage is “6.0 Volt ± 0.1”; if not, turn the TR1 trimmer located on the Vegasys Control Board.;

7.

Remount the assembly by repeating the above step 5 through 1 in reverse order;

8.

Turn the Analyzer on;

9.

Launch “Diagnostic”program;

10.

In order to view the various elements contained under “Diagnostic” program, insert the valid Password within the provided field. Click on “ OK ”;

11.

Select “Diagnostic” button and then, “ Change Photometer Lamp”;

12.

Make sure that the wash solution bottle is filled of proper and fresh solution;

13.

Select “Autozero ”button;

The Vegasys will automatically sample and aspirate wash solution twice, performing the autozero and displaying a chart which states the read value expressed in “ mv” and “Counts ” for each filter.

Acceptable values (in “mv” and “counts ”) are given below (see Table C and D): Table C Filter

Min. Value (mv)

Max. Value (mv)

Dark 340, 405, 492, 510, 546, 578 e 620 Tabella D Filter

Min.Value (Count)

Max. Value (Count)

Dark 340, 405, 492, 510, 546, 578 e 620

If the 340 filter and/o other filters do not present the correct value, before following the set-up procedure for Gain and Offset, check if:

Vegasys



microflow cell is perfectly clean:



microflow cell is full of liquid and that there are no air bubbles on the inside.

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GAIN & OFFSET - ADJUSTMENT Warning: room lighting and daylight can influence the reading

1.

Make sure that the Dark value shows a count value of

XY0 ± Z0; if not, adjust the

Preamp/ADC board “Offset ” trimmer in order to set the desiders value; 2.

Identify which filter transmit the highest signal;

3.

Verify for the filter that transmit the highest signal a count value of ? ± ?; if not, adjust the Preamp/ADC board “Gain” trimmer in order to set the desired value;

4.

Press “Filters Reading”;

5.

Verify for all the filters (from 340 nm to 620 nm) count values between ?? and ??;

6.

Exit from “Diagnostic” program.

CHANGING THE TUBES KIT

Over time and through normal use, the tubes become worn. The manufacturer suggests that the tubes be replaced at least once a year. PROCEDURE FOR REPLACING THE TUBES KIT

1. Turn off the Analyzer; 2. Unlock the four screws of the instrument chassies (make a quarter of turn for each screw); 3. Line up the sampling probe arm to the center of the Reaction Plate; 4. Remove the instrument chassies; 5. Replace the tubes following the indication shown on the “Hydraulic Tubing Diagram”; 6. Turn on the Analyzer; 7. Launch the “Vegasys” software; 8. Launch “Diagnostic”program; 9. In order to view the various elements contained under “ Diagnostic” program, insert the valid Password within the provided field. Click on “OK”; 10. Select “Diluter” button; 11. Carry out eight “PRIME” cycles to make sure that the sampling hydraulic circuit is fil led of liquid; 12. Select “Change Photometer Lamp ” button; 13. Select “Autozero” button to make sure that the aspiration hydraulic circuit is fi lled of liquid; 14. Line up the sampling arm to the center of the Reaction Plate;

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15. Remount the instrument chassies and lock the four screws (make a quarter of turn for each screw); 16. Carry out three “ PRIME” cycles to make sure the sampling hydraulic circuit is filled of liquid; 17. Carry out two “Autozero” cycles to check the system efficiency. If needed, carry out the “Calibration Pump Procedure ”. LIST OF THOSE MAINTENANCE PROCEDURES THAT CAN BE PERFORMED BY THE USER AND/OR THE MAINTENANCA TECHNICAN

PROCEDURE

FREQUENCY (*)

CLEANING THE SAMPLING/ASPIRATION PROBE

DAILY

OPERATOR

CLEANING THE WASH SOLUTION BOTTLE

EVERY TWO WEEKS

OPERATOR

REPLACING THE PROBE CLEANER PAD

200 – 300 TEST

OPERATOR

REPLACING THE PERISTALTIC PUMP TUBE

EVERY SIX MONTHS

OPERATOR

REPLACING THE PHOTOMETER BULB

AFTER 2000 HOURS OF USE

OPERATOR

WASHING MICROFLOW CELL

WEEKLY

OPERATOR

REPLACING THE SAMPLING/ASPIRATION PROBE

AS NEEDED

OPERATOR

CLEANING OF THE HYDRAULIC CIRCUIT

MONTHLY

OPERATOR

REPLACING THE TUBES (Tubes Kit)

ONCE A YEAR

MAINTENANCE TECH

REPLACING THE BELT

AS NEEDED

MAINTENANCE TECH

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EFFECTUATED BY

Page 13


REPLACING A MOTOR

AS NEEDED

MAINTENANCE TECH

ADJUSTING PHOTOMETER

AS NEEDED

MAINTENANCE TECH

AS NEEDED

MAINTENANCE TECH

REPLACING THE ELECTRONIC BOARDS AND THE MECHANICAL MODULES

N.B.: The above – indicated frequency intervals may vary according to the individual instrument’s daily workload.

DECONTAMINATION PROCEDURE

Before replacing any instrument parts, repairing any defective items or performing any instrument maintenance procedure(s), the operator or maintenance technician must carry out the belowdescribed decontamination procedure of the instrument part(s) involved in the operation(s). This procedure can be performed on: 

the entire Analyzer



those part(s) of the instrument subject to possible contamination

NECESSARY MATERIAL

an ESOFENOL solution diluted to 6% (60 cc in one liter of distilled water). ESOFENOL is an antibacterial and antiviral substance.





Rubber gloves



Mask



Lab coat

EXTERNAL SURFACES AND INDIVIDUAL PARTS 

Spray the solution all over the instrument, paying particular attention to wetting:



the sampling arm the reaction plate (including the cuvettes) the racks the instrument chassis Allow the solution to stand for approximately 30 minutes



Wipe the solution off the instrument and the various components using a sponge dampenedwith distilled water

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Carry out a decontamination of the internal hydraulic circuit. HYDRAULIC CIRCUIT (ENTIRE INSTRUMENT) 





Fill a container with a 5% sodium hypochlorite solution; Insert the aspiration cannula, located into the wash solution bottle, into a bottle filled with the 5% sodium hypochlorite solution and have the instrument carry out three “Autozero” cycles; Remove the aspiration cannula from the 5% sodium hypochlorite solution and immerge it in another bottle containing Rinse Solution. or Distilled Water;



Have the instrument carry out three “Autozero” cycles;



Insert the aspiration cannula into its proper bottle.

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Chapter 07 – SETTINGS AND ADJUSTMENTS

CHAPTER 07 - SETTINGS AND ADJUSTMENTS INDEX

SAMPLING AND ASPIRATION ARMS ..........................................................................................2 DILUTER MODULE ..........................................................................................................................9 PHOTOMETER MODULE...............................................................................................................11 PERISTALTIC PUMP MODULE.....................................................................................................16

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SAMPLING AND ASPIRATION ARMS Changing the Sampling Arm

1.

Turn off the “Vegasys” analyzer;

2.

Remove the front casing/chassis, unscrew the 4 holding screws and remove the side casings/chassis;

3.

Remove the sampling probe, first loosening the knurled knob;

4.

Unscrew and remove the screw;

5.

Disconnect the cable;

6.

Remove the old arm;

7.

Insert the new arm, making sure that it is positioned as far down as possible;

8.

Reconnect the cable;

9.

Put the screw back in, but do not over-tighten;

10.

Put the side casings/chassis back on;

11.

Put the sampling probe back in position;

12.

Replace the front casing/chassis;

13.

Put the screw back in but do not over-tighten the knurled knob, in order to allow the sampling probe to move back up if necessary;

14.

Turn the “Vegasys” analyzer back on;

15.

Launch the “Diagnostic” programme;

16.

Select “Arms/Plate Setting” and wait until the system has carried out an instrument reset;

17.

Position the sampling arm over the wash well and tighten it;

18.

Carry out a “General Reset”;

19.

Make sure the sampling probe is balanced and centred over the wash well;

20.

Tighten the sampling arm and repeat the “General Reset”.

21.

Make sure that the sampling probe is still correctly balanced and centred over the wash well (if not, loosen the screws holding the sampling arm in place and repeat steps 19 and 20);

22.

Tighten the knurled knob screw;

23.

Check the downward movement of the sampling probe using the number of preset steps; the optimal downward movement is when the final distance between the top lip of the wash well and the tip of the sampling probe is at least 3 cm (if necessary, correct the number of preset steps);

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24.

Refer to procedure “6.1.1 CENTRING AND ADJUSTMENTS” from step #11 to step # 25;

25.

Exit the “Diagnostic” programme.

Replacing the Aspiration Arm

1.

Turn off the “Vegasys” analyzer;

2.

Remove the front casing/chassis, unscrew the 4 holding screws and remove the side casings/chassis;

3.

Remove the aspiration probe, first loosening the knurled knob;

4.

Unscrew and remove the screw;

5.

Disconnect the cable;

6.

Remove the old arm;

7.

Insert the new arm, making sure that it is positioned as far down as possible;

8.

Reconnect the cable;

9.

Put the screw back in, but do not over-tighten;

10.

Put the side casings/chassis back on;

11.

Put the aspiration probe back in position;

12.

Replace the front casing/chassis;

13.

Put the screw back in but do not over-tighten the knurled knob, in order to allow the sampling probe to move back up if necessary;

14.

Turn the “Vegasys” analyzer back on;

15.


16.

Select “Arms/Plate Setting” and wait until the system has carried out an instrument reset;

17.

Manually position the aspiration arm over the cuvette in the Reaction Plate making sure that it is perfectly centred;

18.

Tighten the aspiration arm;

19.

Carry out a “General Reset”;

20.

Check that the aspiration probe is perfectly centred and balanced over the Reaction Plate cuvette;

21.

Tighten the aspiration arm and repeat a “General Reset”;

22.

Check the downward movement of the aspiration probe using the number of preset steps programmed for the first dip of the aspiration probe into the Reaction Plate cuvette;

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23.

Make sure that the aspiration probe is still correctly positioned (if not, adjust the positioning of the aspiration probe, centring it with respect to the reaction cuvette opening by using the shift buttons below the “GO Cuvette – Aspiration ” button

(green

buttons used to adjust the position of the Reaction Plate with respect to the Aspiration Arm). 24.

Select “Vertical Reset”;

25.

Regulate the descent of the aspiration probe using the first “Down” button making sure that the distance between the bottom of the Reaction Plate cuvette and the tip of the aspiration probe is approximately 27 mm. If the distance measured is not acceptable, use the shift buttons

(yellow

buttons) placed below the first “Down” button to change the number of steps; N.B.: this initial calibration of the descent of the aspiration probe into the reaction cuvette is

carried out with the purpose to insure that the flow cell is correctly primed; 26.

Carry out a second setting of the descent of the aspiration probe using the second “Down” button, making sure that the distance between the bottom of the Reaction cuvette and the tip of the aspiration probe is approximately 1 mm. If the distance measured is not acceptable, use the shift buttons

(yellow

buttons) placed below the second “Down” button to change the number of steps;

N. B.: this second calibration of the descent of the aspiration probe into the reaction cuvette is

carried out with the purpose to insure that all the liquid is aspirated and which, in any case, must not be less than xx µl.

27.

Tighten the knurled knob;

28.

Perform a “Vertical Reset”

29.


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Adjusting the Aspiration and Sampling Arms

1.

Turn on the Vegasys system and launch the instrument management programme by double clicking on the “ Vegasys” icon;

2.

Select “Diagnostic”;

3.

Insert the required password in the “Password Request” window and then confirm by clicking on “OK”;

4.

Select “Diagnostic” in the mask that opens and wait until the instrument has carried out the “Reset” function;

5.

Click on “Arms/Plate Setting”;

6.

Enter the “User Name” and “Password” in the window that opens and confirm by clicking on OK ;

7.

Make sure that the sampling probe is properly centred with respect to the wash well; if not, loosen the arm’s holding screw and mechanically align it over the wash well so that it is properly centred;

INSERIRE FIGURA

8.

Tighten the sampling arm’s holding screw;

9.

Carry out a “General Reset” and once again check that the probe is properly centred over the wash well. If not, repeat steps 7 and 8; After having mechanically centred the sampling probe over the wash well, regulate all the other positions using the buttons included in the mask, as described in the following instructions:

10.

Select “Wash Well”; regulate the descent of the sampling probe into the wash well using the “Down” button making sure that the distance between the top lip of the wash well and the tip

of the probe is at least 3 cm. If the distance measured is not acceptable, use the shift buttons (yellow buttons) to change the number of steps; 11.

Select the “GO Reagent” button; the sampling probe will automatically position itself over the reagent in position # 1;

12.

Regulate the descent of the sampling probe using the “Down” button, making sure that the distance between the bottom of the reagent container and the tip of the sampling probe is 1.2 mm ± 0.2 mm .

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If the distance measured is not acceptable, use the shift buttons

(yellow

buttons) to change the number of steps; 13.

If necessary, regulate the centring of the sampling probe with respect to the reagent container opening by using the buttons placed above and below the “ GO Reagent” button; (pink shift buttons – used to adjust the position of the Sampling Arm with respect to the Reaction Plate) (green shift buttons used to adjust the position of the Reaction Plate with respect to the Sampling Arm);

14.

Regulate the upward movement of the sampling probe by using the “Up” button, making sure that the tip of the sampling probe comes out of the cleaning pad by “ 1 mm”. If

necessary,

regulate

this

distance

by

using

the

violet

coloured

shift

buttons

to change the number of steps; 15.

Under the “GO Reagent” button, select option “ 10”.

16.

Click on “GO Reagent”, the sampling probe will automatically place itself in position # 10;

17.

Regulate the descent of the sampling probe by using the “Down” button, making sure that the distance between the bottom of the reagent container and the tip of the sampling probe is 1.2 mm ± 0.2 mm.


(yellow


If necessary, regulate the centring of the sampling probe with respect to the reagent container opening by using the buttons placed above and below the “ GO Reagent” button; (pink buttons – used to adjust the position of the Sampling Arm with respect to the Reaction Plate) (green buttons used to adjust the position of the Reaction Plate with respect to the Sampling Arm);

19.

Select the “GO Sample” button, the sampling probe will automatically position itself over the first sample in the first samples rack;

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20.

Regulate the descent of the sampling probe by using the “Down” button, making sure that the distance between the bottom of the sample cup and the tip of the sampling probe is 1.2 mm ± 0.2 mm.


(yellow


If necessary, regulate the centring of the sampling probe with respect to the sample cup opening by using the buttons placed above and below the “ GO Sample” button; (pink shift buttons – used to adjust the position of the Sampling Arm with respect to the Reaction Plate) (green shift buttons – used to adjust the position of the Reaction Plate with respect to the Sampling Arm);

22.

Regulate the upward movement of the sampling probe by using the “Up” button, making sure that the tip of the sampling probe comes out of the cleaning pad by “ 1 mm”. If

necessary,

regulate

this

distance

by

using

the

violet

coloured

shift

buttons

to change the number of steps; 23.

Select the “GO Cuvette - Sampling” button, the sampling probe will automatically position itself over the first cuvette of the first reaction sector;

24.

Regulate the descent of the sampling probe by using the “Down” button, making sure that the distance between the bottom of the reaction cuvette and the tip of the sampling probe is 38 mm.


(yellow


If necessary, regulate the centring of the sampling probe with respect to the reaction cuvette opening by using the buttons placed above and below the “ GO Cuvette – Sampling” button: (pink buttons – used to adjust the position of the Sampling Arm with respect to the Reaction Plate) (green buttons – used to adjust the position of the Reaction Plate with respect to the Sampling Arm).

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N. B.: the following steps have to do with regulating the aspiration arm in the reaction cuvette.

Before allowing the aspiration probe to dip into the reaction cuvette, make sure that the aspiration arm is perfectly aligned with the reaction cuvette. This is absolutely necessary in order to ensure that the aspiration probe does not come in direct contact with or rub the lip, sides or edges of the reaction cuvette.

26.

Select the “GO Cuvette – Aspiration” button; the first cuvette of the first reaction sector will automatically position itself directly under the aspiration probe;

27.

Regulate the descent of the aspiration probe by using the first “Down” button, making sure that the distance between the bottom of the Reaction Plate cuvette and the tip of the aspiration probe is approximately 27 mm. If the distance measured is not acceptable, use the shift buttons

(yellow

buttons) placed below the first “Down” button to change the number of steps; N.B.: this initial calibration of the descent of the aspiration probe into the reaction cuvette is

carried out with the purpose to insure that the flow cell is correctly primed. If necessary, regulate the centring of the aspiration probe with respect to the reaction cuvette opening by using the buttons placed below the “ GO Cuvette - Aspiration” button: (green buttons used to adjust the position of the Reaction Plate with respect to the Aspiration Arm; 28.

Carry out a second setting of the descent of the aspiration probe by using the second “Down” button, making sure that the distance between the bottom of the reaction cuvette and the tip of the aspiration probe is approximately 1 mm. If the distance measured is not acceptable, use the shift buttons

(yellow

buttons) placed below the second “Down” button to change the number of steps;

N. B.: this second calibration of the descent of the aspiration probe into the reaction cuvette is

carried out with the purpose to ensure that all the liquid is aspirated – said liquid must, in any case, not be less than xx µl.

Changing the Sampling Arm Motor

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DILUTER MODULE

Changing the diluter module

N.B.: The instrument (Vegasys) must be turned off during the execution of the following procedure.

1.

Loosen the four screws that hold the instrument casing/chassis in place (a quarter turn each);

2.

Align the sampling arm directly over the centre of the Analysis Plate (this makes it possible to remove the instrument casing/chassis);

3.

Remove the instrument casing/chassis;

4.

Disconnect the J? and J?? connectors from the control board;

5.

Remove the two Teflon tubes positioned along the side of the diluter by unscrewing their clamps. Be careful of leakage;

6.

Unscrew the two screws on the base that supports the diluter (Fig. X);

7.

Unscrew the four screws that fasten the dilutor block to its support base;

8.

To remount the diluter, repeat the above four steps in inverse order – or rather: perform steps 7, 6, 5, and 4 in this order.

9.

After remounting, carry out the mechanical functional check as described in section XX

Replacing the diluter electro-valve

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N.B.: The instrument (Vegasys) must be turned off during the execution of the following procedure.

1.

Loosen the four screws that hold the instrument casing/chassis in place (a quarter turn each);

2.

Align the sampling arm directly over the centre of the Analysis Plate (this makes it possible to remove the instrument casing/chassis);

3.

Remove the instrument casing/chassis;

4.

Disconnect the J? connector from the control board;

5.

Remove the Teflon tubes from their respective housings in the electro-valve;

6.

Unscrew the two screws that fasten the diluter, take it out and put in its replacement;

7.

To remount, repeat the above six steps in inverse order – or rather: perform steps 6, 5, 4, 3, 2 and 1 in this order;

8.

After remounting, carry out the mechanical functional check as described in section XX

Mechanical functional check of the diluter

1. Launch the “Diagnostic” programme from System Monitor; 2. Select the “Diagnostic” button and wait until the instrument has carried out a reset; 3. Select “Arms/Plate Setting” ; 4. Enter the user name and password in the “Enter User Name and Password” window; 5. Select the “Free Casing/Chassis” button; Wait until the sampling arm has positioned itself directly over the centre of the Reaction Plate; 6. Loosen the four screws that hold the instrument casing/chassis in place (a quarter turn each); 7. Remove the instrument casing/chassis; 8. Select the “General Rest” button and wait until the arm has positioned itself over the wash well; 9. Select “Dilutor”; 10. Select first the aspiration and then the dispense buttons for volumes between 3 and 500 µl; 11. Check to make sure that the diluter does carry out the requested aspiration and dispense (i.e. watch to ascertain that the required movements are performed). Check for leakage from the hydraulic connections; 12. Exit the diagnostic programme by clicking on “Exit”.

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Mechanical functional check of the electro-valve diluter

1. Launch the “Diagnostic” programme from System Monitor; 2. Select the “Diagnostic” button and wait until the instrument has carried out a reset; 3. Select “Diluter”; 4. Select the “Electro-valve Test” button and make sure that the acoustic alarm on the control board beeps; 5. Click on “Exit”.

PHOTOMETER MODULE

Replacing the photometer lamp

Figure X – Optics – N.B.: The instrument (Vegasys) must be turned off during the execution of the following procedure

To replace the lamp proceed as follows: 

Remove the photometer’s protective cover;



Loosen the screws that hold the lamp’s “A” terminals and disconnect them;



Unscrew the lamp’s support base screw “B” and take out the lamp;



Insert the new lamp without touching the bulb and tighten the “B” screw to block it;



Reconnect the lamp’s “A” terminals and carefully tighten the screws that hold them;



Replace the photometer’s protective cover;



Carry out an electronic regulation of the photometer as described in section XXX

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Electronic regulation of the photometer Warning: the following required procedure must be carried out for both new lamps and new

reaction sectors.

1.

Turn on the Vegasys system and launch the software management programme by double clicking on the “Vegasys” icon;

2.

Launch the diagnostic programme and wait until the instrument has carried out a reset;

3.

Select “Change Photometer Lamp”;

4.

Check to make sure that the voltage present on the lamp wires’ connecting terminal is 6.0 volt ± 0.1; if not, rotate the TR1 trimmer located on the Vegasys control board;

5.

Make sure that the required bottle is full (distilled water) and that there is no trace of dust or mould in the bottle that could impair instrument functionality;

6.

Select the “Auto-zero ” button;

The Vegasys will automatically sample and aspirate the distilled water 3 times – Auto-zeroing – the results expressed in “mv” and “counts” are reported in a table that can be viewed on the screen.

INSERIRE UNA IMMAGINE VALIDA; INOLTRE IL CAMPO DENSITA’ OTTICA E IL GRAFICO NON DEVENO ESSERE PRESENTI.

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The below illustrated tables contain the acceptable range of values as expressed in “mv” and “counts”.

Table A Filter Dark

Minimum Value (mv)

Maximum Value (mv)

340, 405, 492, 510, 546, 578 and 620

Table B Filter

Minimum Value (Count)

Maximum Value (Count)

Dark 340, 405, 492, 510, 546, 578 and 620

In the event that the 340 filter and/or other filter(s) are out of range, before regulating either the Gain or Offset, make sure that:

7.



the reading cell is perfectly clean;



the reading cell is full of liquid and that there are no air bubbles in it.

Make sure that the counts value for the “Dark” filter is equal to XYZ ± Y0; if not, adjust by using the “Offset” trimmer in the Preamp/ADC board;

8.

Identify which filter (from 340 nm to 620nm) shows the highest energy level;

9.

Make sure that the counts value for this highest energy level filter is between ZZ000 and WW000. If not, adjust by using the “Gain” trimmer in the Preamp/ADC board;

10.

Select the “Filters Reading” button;

11.

Make sure that the counts values for each filter are between XX000 and YY000. If not, adjust by using the “Gain” trimmer in the Preamp/ADC board;

12.


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Replacing and regulating the interferential filters

Warning: before performing this calibration procedure make sure that the reading cell is filled with

distilled water.

N. B.: the “340 nm” interferential filter does not need to be shielded. All the other filters may require adequate shielding in order to obtain for each of them an output signal between “XX000 ÷ YY000” counts.

1. With the instrument turned off remove the outside cover to allow access to the optics module; 2. Remove the protective casing around the optics group; 3. Remove the blocking ring from the “340 nm” filter, in the second position on the filters barrel; 4.

Mount the new filter (“340 nm”) in the second position on the filters barrel with the coloured part facing inwards and then tighten the ring nut.

5. Turn the Vegasys system on, launch the instrument management programme by double clicking on the “Vegasys” icon; 6. Launch the diagnostic programme and wait until the instrument has carried out a reset; 7. Select “Change Photometer Lamp”; 8. In the “Select Filter” field, select the “340 nm” filter; the filters barrel will automatically position the “340 nm” filter along the optical axis; 9. Make sure, via the optical path, that the reading cell is filled with distilled water and that there are no air bubbles in it; 10. Select the “Start Reading” button; 11. Use the “Gain” trimmer, positioned on the amplifier board casing, to bring the counts value reading to ZZ000 counts ± WW counts; 12. Select the “STOP Reading” button; 13. Click on “Arms/Plate Setting”; 14. Select the “Motors Off” button; 15. Remove the blocking ring from the “405 nm” filter, in the third position in the filter barrel; 16. Mount the new filter (“405”) in the third position of the filter barrel with the coloured part facing inwards and without forcing the “405 nm” filter ring block; 17. Select the “General Reset” button and wait until the instrument has carried out a reset; 18. Click on “Change Photometer Lamp”; Vegasys

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19. In the “Select Filter” field, select the “405 nm” filter; the filters barrel will automatically position the “405 nm” filter along the optical axis; 20. Select the “Start Reading” button; 21. Make sure that the counts value reading is between “XX000 ÷ YY000” counts; 22. If the counts value reading is not in the “XX000 ÷ YY000” counts range, proceed as follows: 

Select “Arms/Plate Setting”;



Click on the “Motors Off” button;



Remove the required filter’s blocking ring;



Insert one or more equalization shields – chosen each time from among the graded percentages of grey available – until the counts value reading for the required filter falls within the “XX000 ÷ YY000” interval;



Replace the blocking ring without forcing it;



Repeat for each required filter steps 17 through 22.

23. Select “Arms/Plate Setting”; 24. Select the “Motors Off” button; 25. Tighten the blocking ring of the filter in question; 26. For each of the remaining filters (492, 510, 540, 580 and 620) carry out steps 15 through 25 . Please note: following is a list of the positions occupied by the various filters in the optical filters

barrel 1st Position: Dark 2nd Position: 340 nm 3rd Position: 492 nm 4th Position: 510 nm 5th Position: 546 nm 6th Position: 578 nm 7th Position: 620 nm

27. Select the “General Rest” button and wait until the instrument has carried out a reset; 28. Mount the optics group’s protective casing; 29. Select “Change Photometer Lamp” ; 30. Click on “Start Reading” for the “Dark” filter; 31. Make sure that the counts value reading for the “Dark” filter falls within the GGG ÷ HHH interval; if not, use the Offset trimmer on the Preamp/ADC board to bring it within said interval. 32. Select the “Auto-zero” button;

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33. Make sure that the counts value reading for each filter falls within the required interval and if not substitute the relative shield with another having a different graded percentage.

PERISTALTIC PUMP MODULE

Replacing the Peristaltic Pump

Replacing the Peristaltic Pump Motor

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Functional Check of the Peristaltic Pump

1.

Turn on the Analyser;

2.

Launch the “Vegasys” management software;

3.


4.

Enter the required password in the “Password Request” window;

5.

Select “Diluter”;

6.

Select the “PRIME” button;

7.

Make sure that the wash well empties completely, if not make sure that block “B” is as far as it can go towards “D”.

Calibrating the Peristaltic Pump

Warning: place a reagent container filled with distilled water in position R1 of the Analytical Plate.

1. Launch the “Diagnostic” programme; 2. Enter the required password in the “Password Request” window; 3. Select “Peristaltic Pump”; 4. Select the “Start Calibration” button and wait until the liquid dispensed into the reaction cell is aspirated by the aspiration probe;

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5. Make sure that the liquid fills the silicone tubes (going into and coming out of the reading cell), as illustrated in the drawing below (the portion of the silicone tubes that is coloured black);

(B)

(A)

6. In the event that the B portion of the tube shows an excess and the A portion appears insufficiently filled (those portions of the tube that show the liquid entering and exiting the reading cell), decrease the number of steps viewed in the “Final Aspiration” field, select once more “Start Calibration”, and recheck the status of the liquid in the tubes. Vice versa, if there is an excess of liquid in the A portion and the B portion appears insufficiently filled, increase the number of final aspiration steps and then reselect the “Start Calibration” button. Note: if the above described software adjustment process is not sufficiently effective, regulate

the pressure between the silicone tube and the peristaltic pump rotor.

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Chapter 08 – ERROR SIGNALLING

CHAPTER 08 – ERROR SIGNALLING INDEX

8.1

ERROR SIGNALLING ..............................................................................................................2

8.1.1 SYSTEM ERRORS ....................................................................................................................2 8.1.2 RESULTS FLAGS......................................................................................................................3

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8.1 ERROR SIGNALLING This charter is dedicated to a description of the error signalling which may occur during the programming or carrying out of the various operations. Error signals can be divided into the following twogroups:



System Errors



Result Flags

8.1.1 SYSTEM ERRORS

Whenever a system error is detected,it is signalled via a visual text warning message indicating the cause of the signalled anomaly. Following is a list of possible visual text “Warning!” and “Fatal!” messages: “Warning” messages 

Temperature Out of Range



Autozero Out of Range



Host Serial Port Link Missing

“Fatal” messages 

Vertical Sampling Arm Error



Horizontal Sampling Arm Error



Vertical Aspiration Arm Error



Filter Drum Error



ADC Error



Plate Error



Diluter Error



Time Out Error (Generic Error)

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8.1.2 RESULTS FLAGS

Every Result Flag, signalling an error, is accompanied by a symbol. Following is a list of possible flags/indicators:

Flag/Indicator

Description

Fit

Indicates the linearità of the plot of the measured absorbance against time for the sample. Fit = 1.0 is ideal. This means perfect linearity. Fit < 1.0 can also be acceptable. Check the graph.

R I S

*

H

L E

%

Vegasys

R eagent Blank Level value over the limits setup in the Method

Inversion. Reaction direction not in line with that set-up in the Methods. Depletion Limit. Value over the limit set-up in the Methods. Concentration value over the “Lin. Limit High” set-up in the Methods. Concentration value over the max. Normal Value set-up in the Method. Concentration value below the min. Normal Value set-up in the Method. This symbol appears whenever the user has manualy modified he obtained results. This operation annuls all the symbols indicating errors which, in this case, will not be viewed. Coefficient of Variation Percentage in the Standard Replicates over the set value.

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Chapter 09 - INTERFACING PROCEDURES

CHAPTER 09 - INTERFACING PROCEDURES

INDEX

9.1

CONNECTION CABLE........................................................................................................... 2

9.2

CONNECTION PARAMETERS ............................................................................................. 2

9.3

SOFTWARE PROTOCOL ....................................................................................................... 3

9.3.1 FRAME FORMAT ................................................................................................................... 3 9.3.2 BEGIN OF FRAME.................................................................................................................. 3 9.3.3 IDENTIFICATION CODE OF FRAME .................................................................................. 3 9.3.4 DATA FIELD ........................................................................................................................... 4 9.4

PROCEDURE FOR CONTROL DATA TRANSMISSION.................................................... 6

9.4.1 CONNECTION PHASE (DATA LINK) .................................................................................. 6 9.4.2 DATA TRANSFER .................................................................................................................. 6 9.5

TRANSFER OF PATIENTS REQUESTS: HOST ────> AU.............................................. 7

9.6

TRANSFER OF RESULTS: AU ───> HOST........................................................................ 8

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Vegasys can be connected via a host computer to facilitate patient test requisition and reporting. This chapter deals on interfacing procedure which can be utilised in making the connection.

9.1 CONNECTION CABLE Figure 16 shows the pin configuration of serial port RS 232 defining the hardware connection.

VEGASYS DB 25 F

HOST DB 25 F

DB

2 Txd → Rxd

3

2

3 Rxd

Txd

2

3

SG

7

5

7 SG

←

→

9

FIGURE 16

9.2 CONNECTION PARAMETERS Figure 17 shows the parameters for the communication:

• • • • •

BAUD RATE DATA BITS PARITY CHECK STOP BITS END CODE

4800 7 E 2

FIGURE 17 • • • • • •

Vegasys

SOH STX ETX ACK NACK CKS

: BEGINNING OF CONNECTION (POLLING) (01H) : BEGINNING OF FRAME : E ND OF FRAME : CONFIRMATION OF RECEPTION OF FRAME : WRONG RECEPTION OF FRAME : CHECK SUM

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9.3 SOFTWARE PROTOCOL 9.3.1 FRAME FORMAT

Each FRAME transmitted by AU (analyser) and by HOST (computer) is composed of the following elements:

BEGIN OF FRAME

FRAME IDENT. CODE

DATA FIELD

END OF FRAME

Control characters such as (SOH, ACK and NACK) will be transmitted individually. 9.3.2 BEGIN OF FRAME

BEGIN OF FRAME

BEGIN OF FRAME

FRAME IDENT. CODE

DATA FIELD

END OF FRAME

: STX (O2H) length 1 byte

Each FRAME transmitted by AU and/or by HOST will begin with (STX = O2H). 9.3.3 IDENTIFICATION CODE OF FRAME

BEGIN OF FRAME

FRAME IDENT. CODE

BEGIN OF FRAME

DATA FIELD

END OF FRAME

: See Types of Frame below

The identification code of FRAME represents the type of information that will be transmitted. TYPES OF FRAME N. 1 2 3

CHARACTER P R D

ASCII CODE 50H 52H 44H

TRANSMITTER HOST AU AU

No. 1: Character “P“ identifies the transfer of patients from HOST to AU. No. 2: Character “R“ identifies the transfer of results from AU to HOST. No. 3: Character “D“ identifies the transfer of number of patients existing on AU.

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9.3.4 DATA FIELD

BEGIN OF FRAME

DATA FIELD

FRAME IDENT. CODE

DATA FIELD

END OF FRAME

: See the following section

The element “DATA FIELD” is defined depending on the type of identification code of frame: a) IDENTIFICATION CODE OF FRAME = P

DATA FIELD (P)

= length 88 bytes

b) IDENTIFICATION CODE OF FRAME = R DATA FIELD (R)

= variable length

c) IDENTIFICATION CODE OF FRAME = D DATA FIELD (D)

= length 3 bytes

The composition of DATA FIELD (P)

ITEM

= length 88 bytes:

LENGTH

FORMAT

IDENTIFICATION CODE OF PATIENT

8

ALPHANUMERIC

NAME

30

ALPHANUMERIC

TESTS

50

NUMERIC: rrrr....rrr r = 0 -1 0 = without request 1 = with request

FIGURE 18 NOTE: THE VALUE '1' OF TEST FIELD ENABLES THE REQUEST OF THE TEST THAT OCCUPIES THE SAME POSITION IN THE SCHEDULE OF AU. The composition of

Vegasys

DATA FIELD (R) = variable length

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ITEM

LENGTH

FORMAT

8

ALPHANUMERIC

NAME

30

ALPHANUMERIC

N° TEST (1)

2

NUMERIC : range = 1-50 * position of test Schedule of AU

RESULT (1)

8

ALPHANUMERIC

N° TEST (n)

2

NUMERIC

RESULT (n)

8

ALPHANUMERIC

IDENTIFICATION CODE OF PATIENT

1 = with request FIGURE 19

NOTE : THE AU WILL ONLY SEND TESTS WHICH HAVE A RESULT.

The composition of DATA FIELD (D) = length 3 bytes

ITEM

LENGTH

FORMAT

NUMBER OF EXISTING PATIENTS INTO ANALYZER

3

NUMERIC range : 1-100

FIGURE 20

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9.4 PROCEDURE PROCE DURE FOR CONTROL DAT DATA TRANSMISSION 9.4.1 CONNECTION PHASE (DATA LINK) TIME OUT = 3 min. 5 sec.

5 sec.

SOH

5 sec.

SOH

SOH

ºººººººººº

SOH

AU ACK HOST

FIGURE 21 In order to proceed to connection, AU will start a phase of polling (sending of character SOH within intervals of 5 sec) and will remain waiting for the confirmation character ACK. If the confirmation occurs before the last 3 minutes, AU will send the character ACK (link made), otherwise there will be a TIME OUT (disconnection). 9.4.2 DATA TRANSFER

TIME OUT = 30 sec.

FRAME

FRAME

FRAME

ººººººººº

EOT

AU/ HOST ACK

ACK

ACK

HOST/ AU

FIGURE 22 During the transmission phase, TIME OUT is set to 30 sec after which there will be the INTERRUPTION OF COMMUNICATION. Each work session will end with the character EOT. If, during the line connection, there would be a “ NO- ACKNOWLEDGEMENT OF FRAME “ the HOST/AU will reply with a NACK and remain waiting for a second transmission of FRAME up to a maximum of three times. Then, if the FRAME is not received in the right way, the INTERRUPTION OF COMMUNICATION will intervene.

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Service Manual

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08 February 2007

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9.5 TRANSFER OF PATIENTS REQUESTS: HOST ──── > AU AU

:D000CKS

HOST

:

HOST

:CKS

AU

:

HOST

:PID^PAT2^PATIENT^N.2^^^^^^^^^^^^^^^^^^0000000000 01010000000000000000000000000000000000CKS

AU

:

HOST

:PID^PAT3^PATIENT^N.3^^^^^^^^^^^^^^^^^^100010000 000100000000000000000000000000000000000CKS : :

AU HOST

Text format for this case (Transfer of patients requests): D000 CKS D 000 CKS PID^PATIENT^N.1^^^^^^^^^^^^^^^^^ ^1100101000 0000000000000000000000000000000000000< EXT>CKS P ID^PAT1^ PATIENTS^N.1^^^^^^^^^^^^^^^^^^ 11001010000000000000000000 CKS

( transmitted by AU ) FRAME start FRAME identification agent Number of existing patients on AU End of FRAME Check sum (transmitted by HOST) Confirm. of right reception of frame (transmitted by Host)

FRAME start FRAME identification agent PATIENTS identification agent Name of patient Requested tests: 1 - 2 - 5 - 7 of AU schedule End of frame Check sum ( transmitted by AU ) Confirm. of right reception of FRAME

NOTE: Character “^“ = (20H)

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Service Manual

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08 February 2007

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9.6 TRANSFER OF RESULTS: AU ─── > HOST AU

:RID^PAT1^PATIENT^N.1^^^^^^^^^^^^^^^^^^0127^^^^^^ 0231^^^^^^051.2^^^^^07160^^^^^CKS

HOST

:

AU

:RID^PAT2^PATIENT^N.2^^^^^^^^^^^^^^^^^^1238^^^^^^ 14160^^^^^CKS

HOST

:

AU

RID^PAT3^PATIENT^N.3^^^^^^^^^^^^^^^^^^0127^^^^^^ 051.2^^^^^1238^^^^^^CKS

HOST

:

AU

:

Text format for this case (Transfer of results ) RID^PAT1^N.1^^^^^^^^^^^^^^^^^^0127 ( transmitted by AU ) ^^^^^^0231^^^^^^051.2^^^^^07160^^^^^C KS FRAME start R FRAME identification code ID^PAT1^ PATIENT identification code PATIENTS^N.1^^^^^^^^^^^^^^^^^^ Name of patient 01 Test no. 1 27^^^^^^ Result no. 27 02 Test no.2 31^^^^^^ Result no. 31 03 Test no. 3 1.2^^^^^^ Result no. 1.2 07 Test no. 7 160^^^^^^ Result no. 160 End of FRAME Check sum (transmitted by HOST) Confirm. of right reception of FRAME CHECK SUM DETERMINATION:

ASCII sum of all characters of the FRAME ( excluding STX and ETX ). Module 256 = 0. Example: FOR I =1 TO LEN (FRAMES) CKS! = CKS! + ASC ( MIDS FRAMES, I, 1 ) NEXT I Check sum = RIGHT ("0"+ HEX (256 - (CKS! (MOD 256) ) ), 2 )

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Service Manual

Rev.00

08 February 2007

Page 8

Chapter10 - TROUBLESHOOTING

CHAPTER 10 - TROUBLESHOOTING GUIDE -

PROBLEM THE SAMPLING PROBE DOES NOT ASPIRATE.

POSSIBLE CAUSE/RIMEDY 



THE SAMPLING PROBE DOES NOT DISPENSE THE EASH SOLUTION INTO THE WASHING WELL

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REPEATIBILITY OD RESULTS INSUFFICIENT

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• •

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• 

•

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THE ASPIRATION INTO THE FLOW FLOW CELL IS NOT CONSISTENT



 

THE ASPIRATION ARM DESCENDS ENOUGH BELOW THE LIQUID LEVEL TO CORRECTLY ASPIRATE BUT DOES NOT ASPIRATE

Vegasys

Service Manual

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 

Rev.00

The probe could be obstructed; plunge the probe. If the diluter module works, check if there are squeezed or torn tubing. Chech the valve of the diluter. Check if there is enough wash solution in the bottle. Check all tubing connection. Sampling probe dirthy. Hydraulic leak and/or air bubbles in the hydraulic circuit (sampling). Check sampling probe fit. Check the fit of the hydraulic tubes and their connection: if necessary replace the tubes and/or adjust their connection fittings. Deterioration of the Reagent (s). Substitute the bad reagent(s). Light bulb not stable.. Change the light bulb following the procedure described in Chapter 06 – Maintanance. Check the probe cleaner pad. It may be dirthy. In this case, change the probe cleaner pad. Check the flow cell. Make sure it is clean; if necessary, carry out the procedure described in Chapter 06 – Maintanance. The aspiration probe may be dirthy or obstructed. The tubing may be torn or obstructed. Check that the probe descend enough below the liquid level to correctly aspirate. The silicon tubing of the peristaltic pump may be torn. The peristaltic pump rotori s too tight. The probe may be obstructed or unhooked.

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Chapter10 - TROUBLESHOOTING

THE WASHING WELL IS NT EMPTIED EVEN IF THE PERISTALTIC PUMP WORKS PROPERLY







I VALORI DI AUTOZERO SONO TUTTI INFERIORI A XXXXX COUNT







  



I VALORI DI AUTOZERO SONO TUTTI INFERIORI AI XXX COUNT

THE RESULTS OF THE CONTRLS ARE OUT OF RANGE



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





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Service Manual

Rev.00

The tubing from the washing well to the electro-valve may be torn or not properly connected. Check the electro-valve (of the aspiration circuit). Check the peristaltic pump tubing.

The microflow cell may be empty. Fill it with water. The peristltic pump may not be working properly. Check if tubings need to be changed. Check the aspiration probe and its connection with the microflow cell. The may be some leakage or air bubbles in the micoflow cell, or the probe may be dirty. The wash solution bottle may be empty. Check the peristaltic pump electro-valve. Adjust the aspiration volume; there may be too much or to little that has been aspirated. The microflow cell is dirty or air bubbles get into the cell.

Light bulb nearing the end of its life cycle or premature deterioration. In both cases, change the light bulb following the procedure described in Chapter 07 Maintenance. Make sure that the controls have been analyzed with he methods specified by the manufacturer. Make sure that the settings are correct for the following parameters: wavelenght, temperature, sampling volume, reagent volume, factor. Make sure that the water used to prepare the reagent and controls is either bi-distilled or deionized water. Check reagents, controls and standards Make sure that they have been prepared in accordance with the instructions given by the manufacturer. Check that the recommended wash solution was used and not simply distilled water.

08 February 2007

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Vegasys Service Manual Draft

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