Clinical Chemistry I Tests Table

TEST

METHOD

Trinder’s method GLUCOSE DETERMINATI ON

BLOOD UREA NITROGEN

Folin-wu method Ortho-Toulidine by Dubowski Nelson’s Sumogyi method Modified Barthelot

Trinder-uricase BLOOD URIC ACID Blauch and Koch method Catalase system Bittner method TPTZ (2,4,6tripyridyl-5triazine) by morin PAP(4aminophenazon) by Trinder

REAGENT USE; SUBSTRATE

END PRODUCT MEASURE COLOR; WAVELENGTH

NORMAL REFERENCE, CONVENTIONAL OR TRADITIONAL

CONVERT ION FACTOR

S. I.

CLINICAL SIGNIFICANCE

REFERENCE METHOD

-Glucose(Gl.) enzyme reagent; -Gl. Buffer

- Pink color - @510 nm

- 70-110 mg/dL Or - 3.9-6.1 mmol/L

0.05551

mmol/L

Hexokinase  Hyperglycemia  Hypoglycemia To screen for, diagnose, and monitor high blood glucose (hyperglycemia) or low blood glucose (hypoglycemia), diabetes, and prediabetes

-Urea N-base Reagent -Urea N-zyme reagent; -Urea N-color reagent

- green color - @630 nm

- 8-23 mg/dL Or - 2.9-8.2 mmol/L

0.357

mmol/L

Uri-color reagent; Uri-zyme buffer -uricase

- Yellow-orange - @520 nm - @290-300 nm

Male 4-8.5 mg/dL 0.24-0.51 mmol/L

0.05948

mmol/L

-uricase -copper neocuprione (2,4,6-tripyrid-5triazine)

- @410 nm - Deeply colored substance - blue - @590 nm

 Pre-ana Azotemia  Renal azotemia  Post-anal azotemia To evaluate kidney function in a wide range or circumstances, to help diagnose kidney disease, and to monitor patients with acute or chronic kidney dysfunction or failure.  Hyperucemia  Lesh nyan syndrome  Gout The uric acid blood test is used to detect high levels of this compound in the blood in order to help diagnose gout.

-PAP -DHBS

- Red quinonemine derivative - @480-550 nm

Female 2.7-7.3 mg/dL 0.16-0.43 mmol/L

Diacetyl Monoxime by Fearon

Phosphotungstic Acid by caraway

CREATANINE

Folin-wu method Jaffe Reaction

Enzymati/Trinder CHOLESTEROL Salkowski reaction

HDL-C

Dextran sulfate magnesium

0.0114

mmol/L

Van Handell Zilmersmith method

.0259

mmol/L

 Hypertriglycedemia  Hypotriglycedemia Blood tests for triglycerides are usually part of a lipid profile used to identify the risk of developing heart disease. LDL cholesterol is used to predict risk of developing heart disease

µmol/L

Cholesterol enzyme reagent; Cholesterol buffer -HAc -Fe(III)

- pink color - @510 nm

-150-250 mg/dL -3.88-6.47 mmol/L

0.0256

mmol/L

Male 23-63 mg/dL 6.76-16.38 mol/L

.26

Cholesterol enzyme reagent; Cholesterol buffer

Friedewald method

The test for HDL cholesterol (HDL-C) is used along with other lipid tests to screen for unhealthy levels of lipids and to determine your risk of developing heart disease.

88.40

Enzymatic/Trinder

LDL-C

mmol/L

-0.6-1.2 mg/dl -53-106 µmol/L

-acetic anhydrite -H2SO4

GPO-PAP METHOD-RANDOX

Jaffe reaction

- yellow color - @520 nm - creatinine picrate orange red color

LiebermannBurchard reagent

TRIGLYCERIDE

 GFR  Hyperthryoidism  Diabetic acidosis  Puerperium To determine if your kidneys are functioning normally and to monitor treatment for kidney disease  Hypercholestenemia:  CAD  CVD  Hypocholesteronemia  Hemolytic anemia To screen for risk of developing heart disease

Alkaline picrate solution -alkaline picrate solution

Cholesterol enzyme reagent; Cholesterol buffer

Choloestapolyene carbonium ion - red color - choloestapolyene sulfonic acid -green color - Pink color - @520 nm

- Pink color - @500 nm

LDL-C = T.choles.-HDLTG/5 mg/dl

Female 35-75 mg/dl 8.58-19.5 mmol/l -10-190 mg/dL -0.11-2.15 mmol/L

Optimal – <100 mg/dl <2.59 mmol/l

= T.choles.-HDLTG/2.175 mmol/L

Near/above

Liebermannburchard reagent

Friedewald method

optimal - 100-129mgdl - 2.593.34mmol Borderline high - 130-159mg/dl -3.37-4.12mmol/l

TOTAL PROTEIN

ALBUMIN

Biuret method

Bromcresol blue

-

Purple color @545 nm

Bromcresol green

Bromcresol green

-

Lime green color @628 nm

-

BILIRUBIN

Malloy and Evelyn method

Diazo reagent

-

-

Azobiliburin Red violet color @ 530 nm

Very high - >189 - >4.9mol/l - 6-7.8 g/dL - 60-78 g/L

- 3.2-4.5 g/dL Or - 32-45 g/L

-Direct - <0.3 mg/dL; or - <5 µmol/ -Indirect - 0.1-1 mg/dL or - 2-17 µmol/L -Total - 0.1-1.2

10

g/L

10

g/L

17.10

µmol/L

 Hyperproteinemia  Hypoproteinemia Total protein measurements can reflect nutritional status may be used to screen for and help diagnose kidney disease, liver disease, and many other conditions.  Hyperalbuminuria  Hypoalbuminuria To screen for a liver disorder or kidney disease to evaluate nutritional status, especially in hospitalized patients, along with or instead of a pre-albumin test.  Pre-hepatic  Post-hepatic  Hepatic jaundice Bilirubin is measured to diagnose and/or monitor liver diseases, such as cirrhosis, or gallstones

Biuret method

Bromcresol green

Malloy and Evelyn method

-

mg/dL or 2-21 µmol/L

Sources for reference values: Mc Person, R. A., Pincus, M. R. 2011. Henry’s Clinical Diagnosis and Management by Laboratory Methods. Saunders. Burtis, C. A. & Ashwood, E. R. 2001. Tietz Fundamental of Clinical Chemistry. W. B. Saunders Company.