differences between PWD and PAM tender documents.docx
IN THIS DOCUMENT WE GOING TO SEE THE DIFFERENCES BETWEEN HYPERTEXT, HYPERMEDIA AND MULTIMEDIA
This article explain what is the differences between the ASTM A 706/A 706M specification1 for low-alloy steel reinforcing bars and the ASTM A 615/A 615M specification2 for carbon-steel reinforcing ...
differences between PWD and PAM tender documents.docx
Differences between Ethics, Morals and ValuesFull description
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The Differences Between TCM and CCM
This article explain what is the differences between the ASTM A 706/A 706M specification1 for low-alloy steel reinforcing bars and the ASTM A 615/A 615M specification2 for carbon-steel reinf…Descripción completa
Found the several differences between American and British.. here !! published by manusiane
This presentation describes special features of the computer such as: Hypertext, Hypermedia and Multimedia and their examples
nb b
Full description
First to be added Second to be added Volume of inoculum
STREAK PLATE METHOD Nutrient Agar (Melted) A loopful of bacteria from a slant No definite/exact amount (Only a Loopful)
POUR PLATE METHOD Bacterial Broth Nutrient Agar (Melted)
SPREAD PLATE METHOD Nutrient Agar (Melted) Bacterial Broth
1.0 mL or 0.1 mL
0.1 mL Count the number of colonies (number of microorganisms) microorganisms) Petri dish Alcohol Lamp Pipet Test tubes Glass Rod / L-rod
Purpose
For isolation of colonies only
Count the number of colonies (number of microorganisms) microorganisms)
Equipment (Glasswares)
Petri dish Alcohol Lamp Wire Loop
Petri dish Alcohol Lamp Pipet Test tubes
Type of Colonies
Surface Colonies
Advantage
* For isolation of bacterial cultures * Has distinct separate colonies
Disadvantage
* Higher Probability of Contamination Contamination prior to isolation.
Either Surface/ Subsurface Colonies *For Quantification of colonies in solid medium *Allows the growth and quantification of microaerophiles (Sufficient oxygen supply) * Picking subsurface colonies would interrupt other colonies by digging out of the agar * Microbes must withstand the agar temperature
Surface Colonies
* Cultures are never exposed to 45oC melted agar temperatures.
* More microbes, presence of more colony forming units.
References: Culturing Microbes. Microbes . < http://www.mansfield.ohio-state.edu/~sabedon/biol4035.htm> Last Accessed 01 Feb 2010. Growth and Culturing of Bacteria. Last Accessed 01 Feb 2010. Streak Plate Method . < http://www.studentsguide.in/microbiology/microbiology-tools-techniques/streak-plate-method.html> Last Accessed 01 Feb 2010. Pour Plate Method . Last Accessed 01 Feb 2010. Spread Plate Method.< Method.< http://www.studentsguide.in/microbiology/microbiology-tools-techniques/spread-plate-method.html> Last Accessed 01 Feb 2010.
