HEMATOLOGYANALYZER Service Manual
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Tables of Contents Charpter1 using This Manual.................................2 Charpter2 Hardware..............Error! Bookmark not defined. Chapter 3 liquid diagram analysis..........................9 Chapter 4 Histogram and pulse graph..................12 Charpter5 Troubleshouting....Error! Bookmark not defined.
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Charpter1 using This Manual Introduction This chapter explains how to use your HEMATOLOGY ANALYZER service manual, which provides the reference information and procedures needed for servicing your HEMATOLOGY ANALYZER. Read this manual carefully before servicing your analyzer and service your analyzer strictly as instructed in this manual. This manual is to be used in conjunction with the HEMATOLOGY ANALYZER operation manual and does not contain information and procedures already covered in the operation manual.
Who Should Read This Manual This service manual is written for people who Have a thorough understanding of electronic and fluidic principles. Have a thorough understanding of reagent systems. Have a thorough understanding of quality control. Have a thorough understanding of troubleshooting concepts. Have an operator's knowledge of the analyzer. Have the ability to use basic mechanical tools and understand related terminology. Have the ability to use a digital voltmeter (DVM) and an oscilloscope. Have the ability to read electronic and fluidic schematics and understand related terminology.
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Chapter2 Hardware 1. Interface board (1)、the whole block diagram
P104 bus Ë«Ïò
Motor control and drive unit
CPLD--->printer driver
Oneway
CPLD total control unit
Valve drive
WBC-RBC enlarge
CPLD<--- W¡¢R
CPLD<---HGB
Two-way
Analog power supply
Description: (1) CPLD total control unit Control every function module how to communicate with P104 （2）motor control and driver Control motor working and amplify signal （3）electromagnetic valve driver 3
Printer driver
HGB ·enlarge
Amplify the signal of electromagnetic valve driver （4）printer driver Amplify the signal of printer driver。 （5）WBC-RBC amplify unit Amplified WBC-RBC signal that can be more easy to change into digital signal. （6）HGB amplify unit ① Amplified HGB signal that can be easy to change into digital signal； ② Supply power to the HGB lamp。 （7）P104 bus The bus that Connect computer board with CPLD of interface board. （8）analog power supply ① supply power to each part，+48V、+12V、+8V、-8V； ② protect each part supply power 。
(2). The Connector Description printer J51
J47
J39
6 36 4
2 1
J52
J29
3 2 1
2 1
2 1
P104 Bus ...... ...
2
1
110V 1 2
J31
WBC-RBC Amplify sign J48
CPLD Control
Motor Driver
1 2 3
1 2 3 4
1 2 3
J38
V12
V1
2 1
1 2 3 4 1 2 3 4
1 2 3
3 2 1
WASTE
J45
2 1 J30
计量板接入插座 4 3 2 1 J50
DLN
CLEAN
V R8
4 3 2 1
Valve Driver Port
Adjustable Resistor
J43
7 6 5 4 3 2 1 J24 SENS D Port
Adjustable Resistor 3 2 1 VR7
JP4
ASP REAGENT
.......... .......... .......... .......... .......... .......... .......... .......... .......... .......... ..........
SE N S C Po rt
Mo to r C Po rt
Motor D Port
POWER Control
2
JP5
2 4 6 8
V R9
J37
1
Valve Driver
1 3 5 7
Adjustable Resistor
J36
SE N S B Po rt
Mo to r B Po rt
J42
SE N S A Po rt
Mo to r A Po rt
J41
J27
J35
1 2 3
1 2 3
2 1
J40
Adjustable Resistor 3 2 1 VR6 Adjustable Resistor
POWER
LYSE
3 2 1
3 2 1
3 2 1
J32
J33
J34
4
3
2
1
J49
3 2 1
HGB Amplify HGB J44 5 4 3 2 1
VR10
B、explanation (1) Motor and sensor input port (1) J40: motor A （ motor A in related mechanism fasten board module）connection port； (2) J35: the related sensor A of motor A （sensor A in related mechanism fasten board module）input port； (3) J41: motor B （ motor B in related mechanism fasten board module）connection port； (4) J : the sensor of motor B （ the sensor B in the mechanism fasten board）input port； (5) J42: motor C（motor C in the mechanism fasten module）connection port； (6) J37: the sensor C of motor C （ the sensor of the related mechanism fasten board）input port； 4
(7) J43: motor D（ motor D in the mechanism fasten board）connection port； (8):J38: the sensor of motor D(the sensor D in the mechanism fasten board ） input port；
(2) Valve input port (1) J45:valve input port，using valve from V1 to V11
(3) Waste checking input port (1) J30: connect to the waste port
(4) Input port of the flow tubing board (1) J50:the blank line from flow tubing board；
(5) Reagent sensor explanation (1) J32: diluent’s sensor input port (2) J33: Rinse’s sensor input port (3) J34: Lyse’s sensor input port
(6) Liquid tubing input port (1) J24: (7) power input port：J49
(8) Analog power port (1) JP5:when you check the situation of interface board, short-circuit port； (2) JP4:the output connector in the analog power part；
(9) HGB amplify module (1) VR6:HGB signal adjustable resistance； (2) VR10：point of zero voltage adjustable resistance； (3) J44:related measurement cup port (LED and HGB in)；
(10) WBC-RBC amplify module (1) VR7:PLT amplify adjustable resistance； (2) VR8、VR9: adjustable resistance for WBC-RBC-PLT signal； (3) J48:signal input port( WBC-RBC-PLT signal input line)； (4) J31:110V voltage input port； (11) APS indicated lamp port：J29 (12) The drive line of black light port：J39 (13) Port of printer drive board：J47
(14) CPLD control module (1) J27: burn software for 95288 (15) PC104 bus (1) J51、J52: compose with two part
2. Control board (1). The Connector Description
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(二)corresponding port introduction (1) CN15: connect to display； (2) CN14: connect to CF card； (3) CN12: power of control board； (4) CN8、CN9：connect to interface board； (5) CN6: mouse and keyboard port； (6) CN7: connect external parallel port printer from 19 pin (7) CN1: connect buzzer (8) CN17: connect to display (9) CN2: connect serial line (ex: printer) (10) CN11: connect USB mouse and keyboard；
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Chapter 3 liquid diagram analysis According the liquid diagram, there are several kinds of functions. 1. diluent analysis
2. rinse analysis
3. lyse analysis 7
4. test process analysis
5. Waste Analysis
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6. enhanced cleaning
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Chapter 4 Histogram and pulse graph 1. HISTOGRAM There are some practical WBC histograms. (1). Normal WBC histogram
Description: the space between the first line and the second line is lymphocyte; the space between the second line and the third line is intermediate cell; the space between the third line and the fourth line is granulocyte.
(2). Histogram compress too narrow without classification
(3). WBC<0.5, without classification
(4).peak value in the middle, without classification
(5). Nucleated RBC increase or interference or hemolysis insufficiency
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(6) WBC serious interference
(7). without lyse or Hemolytic effect is not good
(8). Increased neutrophils
(9). Increased lymphocytes
(10). Patients with tumor
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(11). Increased monocyte
2. Pulse graph After test, the pulse graph will be saved in the memory, it can help you to analyze the problem. (1) Normal Pulse Graph Select “special”, then input the password: 6666, press “F5” to check WBC pulse graph; press “F6” to check RBC pulse graph; press “F7”to check PLT pulse graph; press “F1” to return to main menu. * The normal range of total pulse for normal people: WBC：< 1M RBC：< 600K PLT：< 1M * the normal range of total pulse for normal QC: WBC：400 ～700K RBC：250～450K PLT：300 ～ 600K Below the normal pulse graph.
WBC pulse graph of normal sample
WBC pulse graph of blank
RBC pulse graph of normal sample
RBC pulse graph of blank
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PLT pulse graph of normal sample
PLT pulse graph of blank
3. abnormal pulse graph
WBC serious interference (Blank)
WBC serious interference (normal sample)
RBC serious interference (Blank)
RBC serious interference (normal sample)
PLT serious interference (Blank) sample)
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PLT serious interference (normal
PLT high-gain (Blank)
PLT high-gain (normal sample)
WBC Slight disturbance (normal sample) not enough lyse or without hemolysis
RBC Slight disturbance (normal sample) high concentration sample for
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RBC
PLT Slight disturbance (normal sample) high concentration sample for PLT
Charpter5 Troubleshouting Problem description WBC、RBC bubble
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reason
Solution
When liquid flow into the metering uint A if there are some bubble in the liquid, B if the liquid flow too fast C if liquid flow into flow sensor , the two LED is always on or the down LED is always on
A: do the function: cleaning B: 1 、according the liquid diagram to check the tubing whether is there leakage 2、enter into submenu :mechanic（password:6800）and check each solenoid valve . C: change the metering unit
WBC and RBC clog
When liquid flow into the metering uint A the liquid stop flowing B the liquid flow along wall C the liquid flow is normal D the liquid flow slowly 。 E the second foot of interface board connector J50 is disconnected with ground
A The most possible is block 1.enhanced flush 2 cauterant the aperture (enter mechanic: password is 6800,) 3 clean the aperture and reassemble the chamber, 4. according the liquid diagram, check the block B use EZ cleaner to clean the metering unit ( put the rinse tube into EZ cleaner, then do the function: enhanced cleaning C check the two LEDs of metering unit, if the LED is always off, change the the board D 1 enhances flush 2 cauterant the aperture (enter mechanic: password is 6800,) 3 clean the aperture and reassemble the chamber 4. according the liquid diagram, check the block E connect the second foot to the ground
WBC or RBC clog
When liquid flow into the metering uint A the liquid flow along wall B the liquid flow is normal C the liquid flow slowly 。
A use EZ cleaner to clean the metering unit ( put the rinse tube into EZ cleaner, then do the function: enhanced cleaning B check the two LEDs of metering unit, if the LED is always off, change the the board C 1 enhances flush 2 cauterant the aperture (enter mechanic: password is 6800,) 3 clean the aperture and reassemble the chamber 4. according the liquid diagram, check the block
5.enter into special(password: 6868), increase the setting time No measuring time
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When liquid flow into the metering uint A the upside LED of metering unit is always off B the the liquid stop flowing
A change the metering unit B the most possible is block
1.enhanced flush 2 cauterant the aperture (enter mechanic: password is 6800,) 3 clean the aperture and reassemble the chamber, 4. according the liquid
diagram, check the block
HGB failure 、HGB bubble
Hemoglobin unstable
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enter into mechanic (password:6800) and check the HGB blank voltage; the normal range 4.25—4.31 A if the voltage is 0V, 1.the HBG lamp is off 2. the HGB photometer is failure B 0< HGB voltage <= 5
A 1. Check the 1 pin of U57, if the voltage is 5V change the HGB lamp; if not, change the interface board 2. reconnecting the receiver line B adjust the resistance VR6 to normal range 4.25—4.31V
A other unstable, B other stable
A check the sampling pump, there is leakage , change the gasket ring B enter into mechanic(password:6800) check the HGB blank voltage fluctuation , if the HGB blank voltage is larger than 0.2V, change the HGB lamp
parameters
are
parameters
are
PLT value is too high in ground test
A ground wire problem B it is shared socket with large efficient machine C whether environment temperature is suit for the request of the machine D the route maintenance :once every week E whether the reagent is expired date or sordid F check every part especially chamber,1. it is rusting，2. the shielding box connect to ground.(how to check it: disconnect the signal wire, check the connection of shielding box and ground
A check the voltage of the groundwire and zero curve If it is less than 5V, it is OK, if it is more than 5V , reconnect ground wire B use a separate socket C environment temperature is suit for the request of the machine D if not, use the function “park” clean all the tubing E change the reagent F change the shielding shim.
PLT unstable
A ground wire problem B it is shared socket with large efficient machine C whether environment temperature is suit for the request of the machine D the route maintenance :once every week
A check the voltage of the ground wire and zero curve If it is less than 5V, it is OK, if it is more than 5V , reconnect ground wire B use a separate socket C environment temperature is suit for the request of the machine
E whether the reagent is expired date or sordid F check every part especially chamber,1. it is rusting，2. The shielding box connect to ground.(how to check it: disconnect the signal wire, check the connection of shielding box and ground
D if not, use the function “park” clean all the tubing E change the reagent F changes the shielding shim.
All the items unstable
A ground wire problem B it is shared socket with large efficient machine C whether environment temperature is suit for the request of the machine D the route maintenance :once every week E whether the reagent is expired date or sordid F check every part especially chamber,1. it is rusting，2. the shielding box connect to ground.(how to check it: disconnect the signal wire, check the connection of shielding box and ground G there is leakage in sampling pump
A check the voltage of the ground wire and zero curve If it is less than 5V, it is OK, if it is more than 5V , reconnect ground wire B use a separate socket C environment temperature is suit for the request of the machine D if not, use the function “park” clean all the tubing E change the reagent F changes the shielding shim. G check the sampling pump, and change the gasket ring
Vacuum pump skid and make a big noise
Ａ the vaccum pump move up or down continuously Ｂ the vaccum pump skid Ｃ when the vaccum pump stop moving, the piston come out from vacuum chamber.
Ａ check whether the voltage in
the first foot of connector J36 is 1.2V.if not, change the sensor. Check the voltage of three foot in connector, when the sheet metal enters into sensor, the voltage is 4.0-5.0V; when the sheet metal is out of sensor, the voltage is 0.01.0V. if not ,change it B changes the rack and the gear. C the waste tubing flow disfluency, check the block place and clean it.
The value of A if the value of HGB is zero, A prime lyse, or change the piston WBC is “*” or the lyse is not enough in the gasket ring of sampling room, tube. B if the total pulse of WBC less “0” B If the value of HGB is not zero, after test QC or sample 18
than 5000, change the interface board
RBC no value
check the pulse of RBC,RBC,PLT(enter into special, the password is 6666 (press F5 to check the pulse of WBC) C enter into mechanic (password: 6800), check the function “sampling” “break”
If the total pulse of WBC is too high, adjust the potentiometer VR8 clockwise If the total pulse of WBC is too high , adjust the potentiometer VR9 anticlockwise C if the function is ok, try to reconnect CONTROL BOARD, if it is failed , change the interface board
A after test QC or sample check the pulse of RBC, (enter into special, the password is 6666 (press F6 to check the pulse of RBC) B enter into mechanic (password: 6800), check the function “sampling” “break”
A if the total pulse of WBC less than 5000, change the interface board If the total pulse of WBC is too high, adjust the potentiometer VR8 clockwise If the total pulse of WBC is too high, adjust the potentiometer VR8 anticlockwise. B if the function is ok, try to reconnect CONTROL BOARD, if it is failed , change the interface board
PLT no value
A enter into mechanic (password: 6800), check the function “sampling” “break” B after test QC or sample check the pulse of RBC, (enter into special, the password is 6666 (press F7 to check the pulse of PLT)
A if the function is ok, try to reconnect CONTROL BOARD, if it is failed , change the interface board B if the total pulse of PLT less than 5000, change the interface board If the total pulse of WBC is too high, adjust the potentiometer VR8 clock wisely If the total pulse of WBC is too high, adjust the potentiometer VR7 anticlockwise.
distort no lyse
A check the voltage of 1th pin of J34 ， it is about 1.2V normally B check the voltage of 3th pin ， it is more than 3.0V when the tube is filled with
A if not, the sensor have broken, change it B if the voltage is about “5V” or”0V”, change the sensor. If the voltage is between 2.0V and 3.0V, try to clean the white reagent
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distort no diluent
reagent; it is less than 2.0V when the tube is empty
detector.
A check the voltage of 1th pin of J32 ， it is about 1.2V normally B check the voltage of 3th pin ， it is more than 3.0V when the tube is filled with reagent; it is less than 2.0V when the tube is empty
A if not, the sensor have broken, change it B if the voltage is about “5V” or”0V”, change the sensor. If the voltage is between 2.0V and 3.0V, try to clean the white reagent detector.
distort no rinse A check the voltage of 1th A if not, the sensor have broken, pin of J33 ， it is about 1.2V normally B check the voltage of 3th pin ， it is more than 3.0V when the tube is filled with reagent; it is less than 2.0V when the tube is empty
change it B if the voltage or”0V”, change the voltage is between try to clean the detector.
not be good at classification
A inappropriate magnification B inappropriate sampling C inappropriate store of blood
A use the quality control to adjust the diagram of WBC B check the sampling C check the expiry date, test another new blood
Only blank in the screen
A no display but other function
A check the connector of screen B reconnect the control board or change the CF card or change the control board
is OK B no display and no other function
Full waste alarm
A
alarm if disconnect the
waste sensor B it is OK when it is not use the waste sensor
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is about “5V” sensor. If the 2.0V and 3.0V, white reagent
A try to change the interface baord B clean the waste sensor or change it
